Short Communications

Diversity of Cecal Bacterial Flora in Bamboo Rat (Rhizomyidae) Analyzed by Amplified Ribosomal DNA Restriction Analysis Technology

  • ZHOU Jianbo ,
  • JIANG Ya ,
  • NING Junping ,
  • CAO Xiaoyan ,
  • HUANG Yi ,
  • WANG Shichang
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  • Animal Science and Technology of Guangxi University, Nanning 530005, China

Received date: 2013-04-11

  Online published: 2013-09-16

Abstract

This experiment was conducted to study the diversity of cecal bacterial flora in bamboo rat (Rhizomyidae) analyzed by amplified ribosomal DNA restriction analysis (ARDRA) technology. The culture-free technology of molecular biology was used to extract microbial total DNA from the cecum of bamboo rat. The bacteria universal primers F27/R1492 were used to amplify bacteria genes of 16S rDNA so as to build up gene library of 16S rDNA. Two kinds of restriction enzymes, HaeⅢ and HhaⅠ, were used to digest amplification products of positive clones, and then different operational taxonomic units (OTU) sequencing were picked up. Through Blast comparing online, the diversity of cecal bacterial flora in bamboo rat was analyzed. The results showed that the most microbes in cecum of bamboo rat were uncultured or unknown strains which were the selected strains of potential significance in taxonomy, and there were microorganism groups related to lipid metabolism. Meanwhile, the distribution of Lactobacillus, Bacillus, Fusiformis and Paenibacillus polymyxa also existed. In conclusion, Bacillus, Fusiformis and two unknown strains whose clone numbers are more than 20 are dominant strains.

Key words: bamboo rat; 16S rDNA; ARDRA; clone

Cite this article

ZHOU Jianbo , JIANG Ya , NING Junping , CAO Xiaoyan , HUANG Yi , WANG Shichang . Diversity of Cecal Bacterial Flora in Bamboo Rat (Rhizomyidae) Analyzed by Amplified Ribosomal DNA Restriction Analysis Technology[J]. Chinese Journal of Animal Nutrition, 2013 , 25(10) : 2504 -2508 . DOI: 10.3969/j.issn.1006-267x.2013.10.035

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