Effects of Jugular Infusion of Lipopolysaccharide on Concentrations of Protein, Lipid and Amino Acids in Peripheral Blood Circulation of Dairy Cattle

JING Longhui; ZHANG Ruiyang; ZHU Weiyun; MAO Shengyong

doi:10.3969/j.issn.1006-267x.2013.12.018

2013 , Vol. 25 >Issue 12: 2913 - 2919

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.12.018

Ruminant Nutrition

Effects of Jugular Infusion of Lipopolysaccharide on Concentrations of Protein, Lipid and Amino Acids in Peripheral Blood Circulation of Dairy Cattle

JING Longhui ,
ZHANG Ruiyang ,
ZHU Weiyun ,
MAO Shengyong

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College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2013-07-31

Online published: 2013-11-19

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Abstract

This study was conducted to investigate the effects of jugular infusion of lipopolysaccharide (LPS) on the concentrations of protein, lipid and amino acids in peripheral blood circulation of dairy cattle. Three Holstein cows at late lactation period were used in a 3×3 Latin square design. Three jugular infusion doses of LPS (Escherichia coli O111: B4) were 0, 0.4, and 0.8 μg/kg BW, respectively. The experiment was consisted of three periods with 15 days in each period. The results showed as follows: LPS infusion could significantly decrease the concentrations of glucose, albumin, globulin, total protein, total cholesterol, high density lipoprotein and low density lipoprotein (P<0.05), but could significantly increase the concentrations of urea nitrogen and triglyceride in serum of dairy cattle (P<0.05). LPS infusion could significantly decrease the concentrations of α-aminobutyric acid, serine, glycine, proline, threonine, citrulline, isoleucine, arginine and aspartate (P<0.05), but had no significant effects on the concentrations of the other amino acids in plasma of dairy cattle (P<0.05). In conclusion, the jugular infusion of LPS can significantly decrease the concentrations of protein, lipid and partial amino acids in blood of dairy cattle, which suggests that translocated LPS from digestive tract can affect the precursor concentrations of milk protein and lipid through immune response of dairy cattle under the condition of high concentrate.

Key words： dairy cattle; lipopolysaccharide; protein; lipid; amino acid; glucose

Cite this article

JING Longhui , ZHANG Ruiyang , ZHU Weiyun , MAO Shengyong . Effects of Jugular Infusion of Lipopolysaccharide on Concentrations of Protein, Lipid and Amino Acids in Peripheral Blood Circulation of Dairy Cattle[J]. Chinese Journal of Animal Nutrition, 2013 , 25(12) : 2913 -2919 . DOI: 10.3969/j.issn.1006-267x.2013.12.018

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