Feed Science and Technology

Effects of Lactobacillus delbrueckii on Growth Performance, Serum Biochemical Indexes, Immune and Antioxidant Function of Suckling Piglets

  • LI Rui ,
  • HOU Gaifeng ,
  • HUANG Qiyong ,
  • PENG Wei ,
  • SHI Yingjun ,
  • LIU Tong ,
  • GONG Yu ,
  • GAO Huan ,
  • HUANG Xingguo
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  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2013-06-03

  Online published: 2013-11-19

Abstract

he purpose of this experiment was to study the effects of Lactobacillus delbrueckii on growth performance, serum biochemical indexes, immune and antioxidant function of suckling piglets. A total of 16 healthy newborn litters of piglets with similar calving date, parity and litter size were randomly assigned to 2 groups (control and experimental groups) with 8 litters per group and 8 piglets in each litter. At 1 (before colostrum intake), 4, 8 and 15 days of age, 1, 2, 3 and 4 mL of sterile saline or Lactobacillus delbrueckii were successively gavaged to piglets in control and experimental groups, respectively. According to similar body weight, one piglet was selected from each litter to collect blood of anterior vena cava at 7, 14 and 21 days of age. The experiment lasted for 21 d. The results showed as follows: 1) compared with the control group, the average weaning weight and average daily gain in experimental group were significantly increased (P<0.01), and diarrhea rate and mortality rate were significantly decreased (P<0.01). 2) At 7 days of age, the activities of alanine aminotransferase (ALT) and aspartate amino transferase (AST) in serum in experimental group were significantly higher than those in control group (P<0.01), and there were no significant differences in the activities of alkaline phosphatase (ALP) and alpha-amylase (α-AMY) between the two groups (P>0.05); at 14 days of age, compared with control group, the activities of ALP, AST and α-AMY in serum in experimental group were significantly increased (P<0.05), and there were no significant differences in the activity of ALT and the content of urea (Urea) between the two groups (P>0.05); at 21 days of age, the activity of AST and the content of Urea in serum were significantly different between the two groups (P<0.05), and there were no significant differences in the other indexes (P>0.05). 3) At each age, compared with control group, the contents of total protein and albumin in serum in experimental group had increasing trends (P>0.05); at 7 and 21 days of age, the content of immunoglobulin G in serum in experimental group was significantly increased (P<0.05); at 14 and 21 days of age, the content of immunoglobulin M in serum in experimental group was significantly increased (P<0.05). 4) Compared with control group, at each age, the total antioxidant capacity in serum in experimental group was significantly improved (P<0.05), while the malondialdehyde content was significantly reduced (P<0.01), as for the activity of superoxide dismutase in experimental group, there was no significant difference (P>0.05); at 7 and 21 days of age, the activity of glutathione peroxidase in serum in experimental group was significantly increased (P<0.05), and there were no effects on the activity of catalase (P>0.05). These results indicate that Lactobacillus delbrueckii can improve the growth performance, immune and antioxidant capacity of suckling piglets.

Cite this article

LI Rui , HOU Gaifeng , HUANG Qiyong , PENG Wei , SHI Yingjun , LIU Tong , GONG Yu , GAO Huan , HUANG Xingguo . Effects of Lactobacillus delbrueckii on Growth Performance, Serum Biochemical Indexes, Immune and Antioxidant Function of Suckling Piglets[J]. Chinese Journal of Animal Nutrition, 2013 , 25(12) : 2943 -2950 . DOI: 10.3969/j.issn.1006-267x.2013.12.022

References

[1] USDA.Part Ⅲ:reference of swine health and environmental management in the United States,2000[Z].USDA:APHIS:VS,CEAH,National Animal Health Monitoring System.#N361.0902,Fort Collins,Co.

[2] CLEMENTE J C,URSELL L K,PARFREY L W,et al.The impact of the gut microbiota on human health:an integrative view[J].Cell,2012,148(6):1258-1270.  

[3] GILL S R,POP M,DEBOY R T,et al.Metagenomic analysis of the human distal gut microbiome[J].Science,2006,312(5778):1355-1359.  

[4] 杨欣,吴雅琨,陈丽仙,等.德氏乳杆菌特性及应用研究进展[J].安徽农业科学,2011,39(8):4724-4726.

[5] NWOKORO O,ANYA F O.Linamarase enzyme from Lactobacillus delbrueckii NRRL B-763:purification and some properties of a β-glucosidase[J].Journal of Mexican Chemical Society,2011,55(4):246-250.

[6] PICCHIETTI S,FAUSTO A M,RANDELLI E,et al.Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.)[J].Fish & Shellfish Immunology,2009,26(3):368-376.  

[7] MAKINO S,IKEGAMI S,KANO H,et al.Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1[J].Journal of Dairy Science,2006,89(8):2873-2881.  

[8] SILVI S,NARDI M,SULPIZIO R,et al.Effect of the addition of Lactobacillus delbrueckii subsp. delbrueckii on the gut microbiota composition and contribution to the well-being of European sea bass (Dicentrarchus labrax,L.)[J].Microbial Ecology in Health and Disease,2008,20(1):53-59.  

[9] 许褆森.德氏乳杆菌对鸡肠道菌群的影响[J].安徽农业科学,2008,36(8):3228-3229.

[10] 文静,孙建安,周绪霞,等.屎肠球菌对仔猪生长性能、免疫和抗氧化功能的影响[J].浙江农业学报,2011,23(1):70-73.

[11] 赵京杨,张金洲.加酶益生素对哺乳及断奶仔猪生产性能和腹泻频率的影响[J].华中农业大学学报,2001,20(2):148-150.

[12] MARK J E,THOMAS G H,ALLEN F H.Effects of antibiotics and probiotics on suckling pig and weaned pig performance[J].The International Journal of Applied Research in Veterinary Medicine,2005,3(4):303-308.

[13] 汪文选,陈琛,卢彤岩,等.恩诺沙星对鲫鱼血液生理生化指标的影响[J].水产学杂志,2009,22(4):20-22.

[14] 姜胜,马昆,于世明,等.小型猪特异性麻醉颉颃剂对小型猪肝肾功能的影响[J].中国兽医杂志,2012,48(1):18-20.

[15] 陈志虹,郭世宁,孔德胜,等.中草药添加剂对育肥猪生长性能及血液生化指标的影响[J].中国兽医学报,2011,31(10):1523-1525.

[16] TREMLETT H,OGER J.Hepatic injury,liver monitoring and the beta-interferons for multiple sclerosis[J].Journal of Neurology,2004,251(11):1297-1303.  

[17] 杨春蕾,李丽娟,周文忠,等.恩诺沙星脂质纳米乳剂对断奶仔猪血液生化指标的影响[J].中国兽药杂志,2012,46(11):20-23.

[18] 宋凡,曹国文,戴荣国,等.复合微生态添加剂对断奶仔猪生产性能与血液生理生化指标的影响[J].饲料工业,2006,27(8):36-38.

[19] 韩杰,边连全,刘显军,等.刺五加多糖对断奶仔猪血液免疫指标的影响[J].动物营养学报,2012,24(12):2444-2449.

[20] MIZUMACHI K,AOKI R,OHMORI H,et al.Effect of fermented liquid diet prepared with Lactobacillus plantarum LQ80 on the immune response in weaning pigs[J].Animal,2009,3(5):670-676.  

[21] 张常明,李路胜,王修启,等.乳酸菌对断奶仔猪生产性能及免疫力的影响[J].华南农业大学学报,2006,27(3):81-84.

[22] 何颖,赵武,秦毅斌,等.NS复合乳酸菌制剂对断奶仔猪免疫水平的影响[J].动物医学进展,2011,32(12):51-55.

[23] 杨静,唐志如.乳酸菌的益生作用机制及其在仔猪生产中的应用[J].饲料研究,2012(12):20-22.

[24] GALDEANO C M,DE LEBLANC A M,VINDEROLA G,et al.Proposed model:mechanisms of immunomodulation induced by probiotic bacteria[J].Clinical and Vaccine Immunology,2007,14(5):485-492.  

[25] 蒋竹英,范觉鑫,陈明洪,等.脱氧雪腐镰刀菌烯醇污染饲粮对断奶仔猪血液生理生化指标的影响及竹炭和竹醋液的干预作用[J].动物营养学报,2012,24(12):2459-2468.

[26] 柳成刚,王晓丽,姜德友,等.肝达康对DMN致大鼠肝损伤血清ALT、AST、TBIL、TP及ALB的影响[J].中医药信息,2012,29(4):74-76.

[27] YANG X J,CHEN J,ZHANG C X,et al.Evaluation of antioxidant activity of fermented soybean meal extract[J].African Journal of Pharmacy and Pharmacology,2012,6(24):1774-1781.

[28] 肖定福,唐志如,印遇龙,等.壳聚糖对大肠杆菌攻毒仔猪生长性能和抗氧化状况的影响[J].激光生物学报,2011,20(6):741-746.

[29] 王全溪,林混,贾洪强,等.乳酸菌素对肉鸡血清生化指标和抗氧化功能的影响[J].动物营养学报,2012,24(1):131-136.

[30] 白明,孟祥晨.益生菌抗氧化活性及菌体抗氧化相关成分的分析[J].食品与发酵工业,2009,3(5):6-11.

[31] RAJPUT I,RASHID L,LI W F,et al.Application of probiotic (Bacillus subtilis) to enhance immunity,antioxidation,digestive enzymes activity and hematological profile of Shaoxing duck[J].Pakistan Veterinary Journal,2013,33(1):69-72.

[32] LI W F,ZHANG X P,SONG W H,et al.Effects of Bacillus preparations on immunity and antioxidant activities in grass carp (Ctenopharyngodon idellus)[J].Fish Physiology and Biochemistry,2012,38(6):1585-1592.  
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