Effects of Dietary &beta;-Glucan on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia-Nitrogen Stress Ability of Japanese Seabass (Lateolabrax japonicus)

WU Chunyu; CAO Junming; HUANG Yanhua; WANG Guoxia; ZHAO Hongxia; LIU Xiaoling; FU Jingjing; LI Lin

doi:10.3969/j.issn.1006-267x.2013.12.034

2013 , Vol. 25 >Issue 12: 3033 - 3040

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.12.034

Short Communications

Effects of Dietary β-Glucan on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia-Nitrogen Stress Ability of Japanese Seabass (Lateolabrax japonicus)

WU Chunyu ,
CAO Junming ,
HUANG Yanhua ,
WANG Guoxia ,
ZHAO Hongxia ,
LIU Xiaoling ,
FU Jingjing ,
LI Lin

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1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Animal Science, Guangdong Academy of Agricultural Science, Guangzhou 510640, China;
3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
4. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2013-06-21

Online published: 2013-11-19

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Abstract

This experiment was conducted to investigate the effects of dietary β-glucan on growth performance, body composition, serum biochemical indices and anti-ammonia-nitrogen stress ability of Japanese seabass (Lateolabrax japonicus). A total of 720 Japanese seabass with the body weight of (8.35±0.17) g were randomly divided into 6 groups with 4 replicates per group and 30 fish per replicate. The fish in control group were fed a basal diet, and the fish in β-glucan supplemental groups were fed the experimental diets supplemented with 0, 200, 400, 600, 800 and 1 000 mg/kg β-glucan on the basis of basal diet, respectively. After 6 weeks feeding, ammonia chloride was used to conduct a ammonia-nitrogen stress test in each group. The results showed as follows: compared with the control group, the weight gain rate and specific growth rate in the β-glucan supplemental groups were significantly increased (P<0.05), and the feed conversation ratio was significantly decreased (P<0.05), but the condition factor, survival rate and hepatosomatic index had no significant difference (P>0.05). No significant differences were found among all groups in the crude protein, crude lipid, ash and moisture contents of whole body (P>0.05). Dietary β-glucan had no significant effects on serum alanine transaminase activity, and high density lipoprotein, low density lipoprotein, cholesterol, triglycerides and urea nitrogen contents (P>0.05). After 96 h ammonia-nitrogen stress, the cumulative mortality rate in the 400 and 600 mg/kg β-glucan supplemental groups was significantly decreased when compared with the control group (P<0.05). In conclusion, dietary β-glucan can increase the growth performance and anti-ammonia-nitrogen stress ability. Using weight gain rate as a evaluation index, the combination of parabola and broken-line regression analysis shows that the optimal supplemental level of β-glucan in the diet of Japanese seabass is 415.10 mg/kg.

Key words： Japanese seabass (Lateolabrax japonicus); β-glucan; growth performance; body composition; serum biochemical indices; ammonia-nitrogen stress

Cite this article

WU Chunyu , CAO Junming , HUANG Yanhua , WANG Guoxia , ZHAO Hongxia , LIU Xiaoling , FU Jingjing , LI Lin . Effects of Dietary β-Glucan on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia-Nitrogen Stress Ability of Japanese Seabass (Lateolabrax japonicus)[J]. Chinese Journal of Animal Nutrition, 2013 , 25(12) : 3033 -3040 . DOI: 10.3969/j.issn.1006-267x.2013.12.034

References

[1] ANDERSON D P.Environmental factors in fish health:immunological aspects[M]//IWAMA G,NAKANISHI T.The fish immune system:organism,pathogen and environment.San Diego,CA:Academic Press,1996:289-310.

[2] 李万坤,闻洪斌,才学鹏,等.β-葡聚糖的免疫增强作用机理的研究进展[J].中国畜牧兽医,2007,34(7):151-155.

[3] 曹敏,陈军,王元春,等.葡聚糖的研究进展[J].广西轻工业,2011(4):17-20.

[4] BAI N,ZHANG W B,MAI K S,et al.Effects of discontinuous administration of β-glucan and glycyrrhizin on the growth and immunity of white shrimp Litopenaeus vannamei[J].Aquaculture,2010,306(1/2/3/4):218-224.

[5] 迟淑艳,周歧存,周健斌,等.β-葡聚糖对奥尼罗非鱼生长性能及抗嗜水气单胞菌感染的影响[J].中国水产科学,2006,13(5):767-774.

[6] 赵红霞,曹俊明,王安利,等.饲料中长期添加β-1,3-葡聚糖对凡纳滨对虾生长性能、体成分和生化指标的影响[J].动物营养学报,2010,22(5):1464-1470.

[7] 张耀武,屈文俊,李文辉.β(1,3)-葡聚糖对锦鲤非特异性免疫功能的影响[J].淡水渔业,2006,36(4):53-55.

[8] MISRA C K,DAS B K,MUKHERJEE S C,et al.Effect of long term administration of dietary β-glucan on immunity,growth and survival of Labeo rohita fingerlings[J].Aquaculture,2006,255(1/2/3/4):82-94.

[9] CHANG J,ZHANG W B,MAI K S,et al.Effects of dietary β-glucan and glycyrrhizin on non-specific immunity and disease resistance of the sea cucumber (Apostichopus japonicus Selenka) challenged with Vibrio splendidus[J].Journal of Ocean University of China,2010,9(4):389-394.

[10] BAI N,ZHANG W B,MAI K S,et al.Effects of continuous and alternate administration of β-glucan and mannan-oligosaccharide on the growth,immunity and resistance against Vibrio splendidus of sea cucumber Apostichopus japonicus (Selenka)[J].Aquaculture Research,2013,44(9).doi:10.1111/are.12055.

[11] EMERSON K,RUSSO R C,LUND R E,et al.Aqueous ammonia equilibrium calculations:effect of pH and temperature[J].Journal of the Fisheries Research Board of Canada,1975,32(12):2379-2383.

[12] 王计刚,曹俊明,赵红霞,等.β-1,3-葡聚糖对凡纳滨对虾生长性能的影响[J].现代渔业信息,2008,23(6):3-5,25.

[13] LÓPEZ N,CUZON G,GAXIOLA G,et al.Physiological,nutritional,and immunological role of dietary β-1,3-glucan and ascorbic acid 2-monophosphate in Litopenaeus vannamei juveniles[J].Aquaculture,2003,224(1/2/3/4):223-243.

[14] 刘立鹤,郑石轩,谭斌,等.不同饲料配方下β-葡聚糖对凡纳滨对虾生长性能、非特异性免疫力的影响及成本分析[J].饲料工业,2005,26(8):26-30.

[15] AI Q H,MAI K S,ZHANG L,et al.Effects of dietary β-1,3-glucan on innate immune response of large yellow croaker,Pseudosciaena crocea[J].Fish & Shellfish Immunology,2007,22(4):394-402.

[16] 张华,方热军.β-葡聚糖对动物的免疫增强和促生长作用及其机理[J].湖南饲料,2009(4):29-31.

[17] 张健.免疫增强剂对凡纳滨对虾幼虾生长、免疫及抗应激的影响[D].博士学位论文.广州:中山大学,2012:82-91.

[18] 李富东,叶继丹,王琨,等.长时间连续投喂β-葡聚糖对鲤生长性能及非特异性免疫功能的影响[J].动物营养学报,2009,21(4):499-505.

[19] SKJERMO J,STORSSETH T R,HANSEN K,et al.Evaluation of β-(1→3,l→6)-glucans and high-M alginate used as immunostimulatory dietary supplement during first feeding and weaning of Atlantic cod (Gadus morhua L.)[J].Aquaculture,2006,261(3):1088-1101.

[20] 蔡成岗,蒋新龙,蒋昌海.β-葡聚糖结构、功能与开发研究进展[J].农产品加工:学刊,2011(9):114-117.

[21] 周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.

[22] BAHMANI M,ORYAN S,POURKAZEMI M,et al.Effects of ecophysiological stress on cellular immunity system of Persian sturgeon Acipenser persicus[R].Tehran:14th Iranian Congress of Physiology and Pharmacology,1999.

[23] 钟巧青,赵水平,董静,等.高密度脂蛋白对炎症状态下脂肪细胞功能的保护作用[J].中国药理学通报,2007,23(7):896-898.

[24] 傅伟龙,江青艳,高萍.动物生理学[M].北京:中国农业科技出版社,2001.

[25] NICOLOSI R,BELL S J,BISTRIAN B R,et al.Changes in plasma lipids from a yeast-derived-glucan fiber in hypercholesterolemic patients[J].The American Journal of Clinical Nutrition,1998(34):189-203.

[26] 徐文.高血脂症与脂肪肝的关系探讨[J].医学信息,2010,23(7):2098-2099.

[27] 吴小兰,刘先洲.脂肪肝与谷丙转氨酶、血糖及血脂的相关性分析[J].公共卫生与预防医学,2007,18(4):10-12.

[28] 周传朋,刘波,王广宇,等.寡糖-中草药复合物和黄霉素对异育银鲫生长、免疫及抗病力的影响[J].淡水渔业,2009,39(3):46-51.

[29] 贺国龙,刘立鹤,乐义详,等.酵母细胞壁免疫多糖对草鱼免疫功能的影响[J].粮食与饲料工业,2010(7):43-45.

[30] 龙章强.黑鲷(Acanthopagrus schlegeli)幼鱼对氨氮胁迫的生理响应及其维生素C的营养需求研究.硕士学位论文.上海:华东师范大学,2008.

[31] PERSON-LE-RUYET J,LAMERS A,LE-ROUX A,et al.Long-term ammonia exposure of turbot:effects on plasma parameters[J].Journal of Fish Biology,2003,62(4):879-894.

[32] CHENG W,HSIAO I S,CHEN J C.Effect of ammonia on the immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus[J].Fish & Shellfish Immunology,2004,17(3):193-202.

[33] RANDALL D J,TSUI T K N.Ammonia toxicity in fish[J].Marine Pollution Bulletin,2002,45:17-23.

[34] SELVARAJ V,SAMPATH K,SEKAR V.Administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (Cyprinus carpio) infected with Aeromonas hydrophila[J].Fish & Shellfish Immunology,2005,19(4):293-306.

[35] 陈乃松,魏涛涛,廖奕招.蝇蛆粉和β-葡聚糖对凡纳滨对虾生长和免疫的影响[J].水产学报,2007,31(6):771-777.

[36] 王雪良.酵母β-葡聚糖对中华绒螯蟹免疫功能的影响[D].硕士专业学位论文.苏州:苏州大学,2008.

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