Effects of Dietary Tryptophan Level on Protein Metabolism of Yangzhou Goslings

YU Lihuai; ZHANG Jun; WEI Zongyou; WANG Hongrong; PAN Xiaohua; LIANG Xianghuan; YANG Xue

doi:10.3969/j.issn.1006-267x.2014.01.012

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 1: 81 - 89

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.012

Poultry Nutrition

Effects of Dietary Tryptophan Level on Protein Metabolism of Yangzhou Goslings

YU Lihuai ,
ZHANG Jun ,
WEI Zongyou ,
WANG Hongrong ,
PAN Xiaohua ,
LIANG Xianghuan ,
YANG Xue

Expand

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 222500, China;
2. Taicang Animal Husbandry &. Veterinary Station, Taicang 215400, China;
3. Kangyuan Dairy Company of Yangzhouhou, Yangzhou 222500, China

Received date: 2013-07-02

Online published: 2013-12-24

Fold

Abstract

This study was conducted to investigate the effects of dietary tryptophan level and feeding regimen on protein metabolism of Yangzhou goslings. A total of two hundred and seventy 21-day-old Yangzhou goslings were used in a 2×3 double factorial design trial and randomly assigned to 6 groups with 3 replicates in each group and 15 goslings in each replicate. Treatments consisted of two feeding regimens (ad libitum and 90% feed restriction), and each contained three dietary tryptophan levels (groupⅠ 0.14%, group Ⅱ 0.22% and group Ⅲ 0.30%). The experiment lasted for 42 days. The results showed as follows: 1) feeding regimen had significant effects on protein deposition content (PDC) in breast and thigh and crude protein (CP) content in liver (P<0.05), the breast PDC and thigh CP content in groups Ⅱ and Ⅲ were significantly increased than those in group Ⅰ (P<0.05), and the interaction between feeding regimen and dietary tryptophan level was significant in the liver CP content (P<0.05). 2) The serum tryptophan/LNAAs (large neutral amino acids) in restricted feeding group Ⅲ was significantly higher than that in group Ⅰ (P<0.05). Compared with group Ⅰ, the serum total protein content in groups Ⅱ and Ⅲ was significantly increased (P<0.05), while the serum uric acid content in group Ⅲ and serum cortisol content in group Ⅱ were significantly decreased (P<0.05). No interaction was found between dietary tryptophan level and feeding regimen in serum biochemical parameters (P<0.05). 3) The protein fractional synthesis rate and fractional growth rate in groups Ⅱ and Ⅲ were significantly higher than those in group Ⅰ (P<0.05). In conclusion, adding tryptophan in low dietary tryptophan level diet can increase the body protein deposition of Yangzhou goslings.

Key words： tryptophan; feeding regimen; protein metabolism; goslings

Cite this article

YU Lihuai , ZHANG Jun , WEI Zongyou , WANG Hongrong , PAN Xiaohua , LIANG Xianghuan , YANG Xue . Effects of Dietary Tryptophan Level on Protein Metabolism of Yangzhou Goslings[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 81 -89 . DOI: 10.3969/j.issn.1006-267x.2014.01.012

References

[1] ANDREW A F, FRANK H, MARKUS R.Estimates of individual factors of the tryptophan requirement based on protein and tryptophan accretion responses to increasing tryptophan supply in broiler chickens 8 to 21 days of age[J].Archives of Animal Nutrtiton, 2005, 59(3):181-190.

[2] ROSA A P, PESTI G M, EDWARDS H M, Jr, et al.Tryptophan requirements of different broiler genotypes[J].Poultry Science, 2001, 80(12):1718-1722.

[3] CORZO A, MORAN E T, Jr, HOEHLER D, et al.Dietary tryptophan need of broiler males from forty-two to fifty-six days of age[J].Poultry Science, 2005, 84(2):226-231.

[4] PONTER A A, CORTAMIRA N O, SEVE B, et al.The effects of energy source and tryptophan on the rate of protein synthesis and on hormones of the entero-insular axis in the piglet[J].British Journal of Nutrition, 1994, 71(5):661-674.

[5] SAWADOGO M L, PIVA A, PANCIROLI A, et al.Marginal efficiency of free or protected crystalline L-tryptophan for tryptophan and protein accretion in early-weaned pigs[J].Journal of Animal Science, 1997, 75(6):1561-1568.

[6] 席鹏彬, 林映才, 蒋宗勇, 等.饲粮色氨酸对43-63日龄黄羽肉鸡生长、胴体品质、体成分沉积及下丘脑5-羟色胺的影响[J].动物营养学报, 2009, 21(2):137-145.

[7] 任建波, 赵广永, 李元晓, 等.日粮色氨酸水平对生长猪的氮利用效率、血浆类胰岛素生长因子-Ⅰ、生长激素及胰岛素的影响[J].动物营养学报, 2007, 19(3):264-268.

[8] MA H, ZHANG W, ZHU X P, et al.Effects of rumen-protected tryptophan on growth performance, fibre characteristics, nutrient utilization and plasma essential amino acids in Cashmere goats during the cashmere slow growth period[J].Livestock Science, 2010, 131(2):227-233.

[9] LANG C H.Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle[J].American Journal of Physiology:Endocrinology and Metabolism, 2006, 291(3):E665-E674.

[10] SUGAWARA T, ITO Y, NISHIZAWA N, et al.Supplementation with dietary leucine to a protein-deficient diet suppresses myofibrillar protein degradation in rats[J].Journal of Nutritional Science and Vitaminology, 2007, 53(6):552-556.

[11] YOSHIZAWA F.Regulation of protein synthesis by branched-chain amino acids in vivo[J].Biochemical and Biophysical Research Communications, 2004, 313(2):417-422.

[12] 施寿荣, 王志跃, 杨海明, 等.日粮能量和蛋白质水平对5-10周龄仔鹅生产性能的影响[J].饲料工业:自然科学版, 2006, 27(23):39-42.

[13] 闵育娜, 侯水生, 高玉鹏, 等.5-8周龄肉鹅能量和蛋白质营养需要量研究[J].西北农林科技大学学报, 2006, 34(12):34-40.

[14] MACKENZIE S L.Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters[J].Journal of the Association of Official Analytical Chemists, 1987, 70(1):151-160.

[15] 宾石玉.日粮淀粉来源对断奶仔猪生产性能、小肠淀粉消化和内脏组织蛋白质合成的影响.博士学位论文.雅安:四川农业大学, 2005:31-34.

[16] GARLICK P J, FERN M, PREEDY V R.The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats[J].Biochemical Journal, 1983, 210(3):669-676.

[17] LIN F D, SMITH T K, BAYLEY H S.A role for tryptophan in regulation of protein synthesis in porcine muscle[J].The Journal of Nutrition, 1988, 118(4):445-449.

[18] PEISKER M, SIMMINS P H.Tryptophan as a feed intake stimulant[J].Feed Mix, 1998, 6(1):8-12.

[19] LI E T S, ANDERSON G H.Amino acids in the regulation of food intake[J].Nutrition Abstracts and Reviews in Clinical Nutrition Series A, 1983, 53:169-181.

[20] HENRY Y, SEVE B, COLLÉAUX Y, et al.Interactive effects of dietary levels of tryptophan and protein on voluntary feed intake and growth performance in pigs, in relation to plasma free amino acids and hypothalamic serotonin[J].Journal of Animal Science, 1992, 70(6):1873-1887.

[21] SAINIO E L, PULKKI K, YOUNG S N.L-tryptophan:biochemical, nutritional and pharmacological aspects[J].Amino Acids, 1996, 10(1):21-47.

[22] 苏有健.在低蛋白日粮中添加色氨酸对仔猪生产性能和下丘脑5-羟色胺水平的影响.博士学位论文.北京:中国农业大学, 2004:28-29.

[23] 饶巍, 王玥, 周斌, 等.日粮色氨酸水平对蛋鸡生产性能及蛋白质代谢的影响[J].中国畜牧杂志, 2011, 47(15):38-41.

[24] 周斌, 李慧, 邹晓庭, 等.色氨酸对产蛋鸡脂肪代谢的影响[J].中国畜牧杂志, 2011, 47(7):50-53.

[25] 马玉娥, 占秀安, 朱巧明, 等.饲粮色氨酸水平对黄羽肉种鸡生产性能、抗氧化功能及血清生化指标的影响[J].动物营养学报, 2011, 23(12):2177-2182.

[26] SIMMONS P S, MILES J M, GERICH J E, et al.Increased proteolysis:an effect of increases in plasma cortisol within the physiologic range[J].Journal of Clinical Investigation, 1984, 73(2):412-420.

[27] GUZIK A C, MATTHEWS J O, KERR B J, et al.Dietary tryptophan effects on plasma and salivary cortisol and meat quality in pigs[J].Journal of Animal Science, 2006, 84(8):2251-2259.

[28] 张括, 王安, 刘洋景.12-17周龄金定蛋鸭色氨酸适宜需要量的研究[J].饲料工业, 2011, 32(20):6-9.

[29] CORTAMIRA N O, SEVE B, LEBRETON Y, et al.Effect of dietary tryptophan on muscle, liver and whole-body protein synthesis in weaned piglets:relationship to plasma insulin[J].British Journal of Nutrition, 1991, 66(3):423-435.

[30] SIDRANSKY H, VERNEY E.Effect of amino acid imbalances on the stimulatory effect of L-tryptophan on hepatic protein synthesis[J].Amino Acids, 1997, 12(3/4):205-212.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References