Effects of Cysteamine on Ruminal Fermentation Parameters and Methane Production of Water Buffalo by in Vitro Gas Production Method

HUANG Yali; ZOU Caixia; WEI Shengju; LIANG Xin; LI Shulu; LU Tianshui; YANG Bingzhuang; LIANG Xianwei

doi:10.3969/j.issn.1006-267x.2014.01.017

2014 , Vol. 26 >Issue 1: 125 - 133

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.017

Ruminant Nutrition

Effects of Cysteamine on Ruminal Fermentation Parameters and Methane Production of Water Buffalo by in Vitro Gas Production Method

HUANG Yali ,
ZOU Caixia ,
WEI Shengju ,
LIANG Xin ,
LI Shulu ,
LU Tianshui ,
YANG Bingzhuang ,
LIANG Xianwei

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1. Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China;
2. Guangxi Agricultural Vocational and Technical College, Nanning 530007, China;
3. Nanjing Agricultural University, Nanjing 210095, China

Received date: 2013-08-19

Online published: 2013-12-24

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Abstract

The current experiment was carried out to investigate the effects of cysteamine (Cs) on ruminal fermentation parameters and methane production of water buffalo by in vitro gas production method. A reading pressure in vitro gas production system was used. The ratio of concentrate to forage of substrate was 32.5：67.5. Cs was supplemented in concentrate at levels of 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0% (dry matter basis), respectively. The results showed as follows: 1) Cs had no significant effect on 72 hours accumulation of gas production (P>0.05); digestible organic matter content and metabolizable energy were the highest in 0.8% Cs group, and significantly higher than those in 0.2%, 0.4% and 0.6% Cs groups (P<0.05). 2) Cs had no significant effect on ammonia nitrogen concentration (P>0.05), but microbial protein concentration in 0.8% Cs group was significantly higher than that in other groups (P<0.05 or P<0.01). 3) Acetic acid concentration in 0.8% Cs group was the highest, but had no significant difference with that of other groups (P>0.05); compared with the control group, acetic acid/propionic acid was significantly increased in 0.6% Cs group (P<0.05). 4) Cs was supplemented in concentrate at a higher level than 0.4% could significantly decrease methane production after 6, 12 and 24 hours fermentation (P<0.05 or P<0.01). The results of in vitro gas production method indicate that Cs can promote rumen microbe fermentation, but decrease methane production of water buffalo when supplemental level of Cs is 0.8%.

Key words： cysteamine; buffalo; rumen fermentation; methane production

Cite this article

HUANG Yali , ZOU Caixia , WEI Shengju , LIANG Xin , LI Shulu , LU Tianshui , YANG Bingzhuang , LIANG Xianwei . Effects of Cysteamine on Ruminal Fermentation Parameters and Methane Production of Water Buffalo by in Vitro Gas Production Method[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 125 -133 . DOI: 10.3969/j.issn.1006-267x.2014.01.017

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