Effects of Dietary Protein and Methionine on Fur Development Related Gene Expressions in Skin of Minks

ZHANG Haihua; LI Guangyu; CHANG Zhongjuan; ZHANG Tietao; ZHONG Wei; ZHOU Ning; SUN Weili; ZHAO Jingbo; YANG Fuhe

doi:10.3969/j.issn.1006-267x.2014.01.023

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 1: 177 - 183

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.023

Special Economic Animal Nutrition

Effects of Dietary Protein and Methionine on Fur Development Related Gene Expressions in Skin of Minks

ZHANG Haihua ,
LI Guangyu ,
CHANG Zhongjuan ,
ZHANG Tietao ,
ZHONG Wei ,
ZHOU Ning ,
SUN Weili ,
ZHAO Jingbo ,
YANG Fuhe

Expand

1. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun 130112, China;
2. Agriculture College, Yanbian University, Yanji 133002, China

Received date: 2013-07-22

Online published: 2013-12-24

Fold

Abstract

This experiment was conducted to investigate the effects of dietary protein and methionine on fur development related gene expressions in skin of minks. Fifty-six male minks aged 125 days were divided into 4 groups (groups A, B, C and D) with 14 replicates per group. Minks in groups A, B and C were fed the diets with 32%, 24% and 16% protein levels, respectively, and those in group D were fed 24% protein level diet with 0.8% DL-methionine supplementation. Four minks with similar body weight from each group were slaughtered to collect the samples of belly skin on day 85. Total RNAs were extracted from the skin tissues, and then insulin-like growth factor-Ⅰ (IGF-Ⅰ), insulin-like growth factor-Ⅰ receptor (IGF-ⅠR), epidermal growth factor (EGF) and β-actin genes were amplified after reverse transcription. Finally, the expressions of IGF-Ⅰ, IGF-ⅠR and EGF genes in mink's skin were analyzed by real-time fluorescence quantitative PCR. The results showed as follows: IGF-Ⅰ, IGF-ⅠR and EGF gene expressions were significantly affected by different dietary protein levels and methionine supplementation in 24% protein diet (P<0.01), those in group A were significantly higher than those in group C (P<0.01), and those in group D were significantly higher than those in groups B and C (P<0.01). The expressions of IGF-Ⅰ and IGF-ⅠR genes in group D were not significantly different from those in group A (P>0.05), but the expression of EGF gene was significantly lower than that in group A (P<0.01). It is concluded that high dietary protein level and low protein diet adding methionine can improve growth and development of fur in minks.

Key words： protein; methionine; mink; gene expression

Cite this article

ZHANG Haihua , LI Guangyu , CHANG Zhongjuan , ZHANG Tietao , ZHONG Wei , ZHOU Ning , SUN Weili , ZHAO Jingbo , YANG Fuhe . Effects of Dietary Protein and Methionine on Fur Development Related Gene Expressions in Skin of Minks[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 177 -183 . DOI: 10.3969/j.issn.1006-267x.2014.01.023

References

[1] BLOMSTEDT L.Pelage development in mink, ferret, and blue fox, and some influence factors[D].Ph.D.Thesis.Finland:Department of Biosciences, University of Helsinki, 1998:36.

[2] 孙海洲, 侯先志, 红梅.内蒙古阿尔巴斯白绒山羊皮肤毛囊发育及其活性变化规律的研究[J].内蒙古畜牧科学, 1998, 19(4):3-6.

[3] 彭玉麟, 贾志海, 卢德勋, 等.不同蛋白质水平的日粮对内蒙古白绒山羊消化代谢的影响[J].畜牧兽医学报, 2002, 33(4):321-326.

[4] RASMUSSEN P V, BØRSTING C F.Effects of variations in dietary protein levels on hair growth and pelt quality in mink (Mustela vison)[J].Canadian Journal of Animal Science, 2000, 80(4):633-642.

[5] GLEM-HANSEN N.Review of protein and amino acid requirements for mink[J].Scientifur, 1992, 16(2):122-141.

[6] MATA G, MASTERS D G, BUSCALL D.Responses in wool growth, live weight, glutathione and amino acids, in Merino wethers fed increasing amounts of methionine protected from degradation in the rumen[J].Australian Journal of Agricultural Research, 1995, 46(6):1189-1204.

[7] TYÖPPÖNEN J, BERG H, VALTONEN M.Effects of dietary supplementation of methionine and lysine on milk parameters and fur quality in blue fox during low-protein feeding[J].Agricultural Science in Finland, 1987, 59:355-360.

[8] ZHANG H H, LI G Y, LIU B Y, et al.Effects of low-protein, DL-methionine and lysine-supplemented diets on growth performance, N-balance and fur characteristics of blue foxes (Alopex lagopus) during the growing-furring period[J].Acta Zoonutrimenta Sinica, 2010, 22(6):1614-1624.

[9] DAHLMAN T.Protein and amino acids in the nutrition of the growing-furring blue fox[D].Ph.D.Thesis.Finland:University of Helsinki, 2003:33.

[10] 杨淑霞, 钟志红, 秦俭, 等.表皮生长因子对游离毛囊生长的影响[J].中华皮肤科杂志, 2000(2):99.

[11] STENN K S, PAUS R.Controls of hair follicle cycling[J].Physiological Reviews, 2001, 81(1):449-494.

[12] 李岩, 廖和荣, 李知勉, 等.IGF-1对不同绵羊皮肤中5个毛囊相关生长因子基因表达的影响[J].中国农业科学, 2007, 40(3):594-600.

[13] VANDEHAAR M J, MOATS-STAATS B M, DAVENPORT M L, et al.Reduced serum concentrations of insulin-like growth factor-Ⅰ(IGF-Ⅰ)in protein-restricted growing rats are accompanied by reduced IGF-Ⅰ mRNA levels in liver and skeletal muscle[J].Journal of Endocrinology, 1991, 130(2):305-312.

[14] PELL J M, SAUNDERS J C, GILMOUR R S.Differential regulation of transcription initiation from insulin-like growth factor-Ⅰ(IGF-Ⅰ)leader exons and of tissue IGF-Ⅰ expression in response to changed growth hormone and nutritional status in sheep[J].Journal of Endocrinology, 1993, 132(4):1797-1807.

[15] LAUTERIO T J, SCANES C G.The role of thyroid hormones in the growth hormone response to protein restriction in the domestic fowl(Gallus domesticus)[J].Journal of Endocrinology, 1988, 117(2):223-228.

[16] ROSEBROUGH R W, MCMURTRY J P.Protein and energy relationships in the broiler chicken.Effects of protein quantity and quality on metabolism[J].British Journal of Nutrition, 1993, 70:667-678.

[17] 荣风梅, 谭兴智.日粮中添加蛋氨酸对生长肉兔肝脏中IGF-Ⅰ mRNA表达量的影响[J].中国动物检疫, 2010, 27(9):39-41.

[18] CAREW L B, MCMURTRY J P, ALSTER F A.Effects of methionie deficiencies on plasma levels of thyroid hormones, insuline-like growth factors-Ⅰ and -Ⅱ, liver and body weights, and feed intake in growing chickens[J].Journal of Poultry Science, 1987, 82:1932-1938.

[19] 李知勉.毛囊相关生长基因的表达对绵羊毛囊生长的影响[D].硕士学位论文.石河子:石河子大学, 2006.

[20] ADAMS N R, BRIEGEL J R, THOMPSON M J, et al.Metabolic hormones and tissue concentrations of mRNA for IGF-Ⅰ in lines of sheep that differ in their protein synthesis response to feed intake[J].The Journal of Endocrinology, 2000, 167(2):315-320.

[21] ROGERS S A, RASMUSSEN J, MILLER S B, et al.Effects of growth hormone on rat renal epidermal growth factor expression[J].American Journal of Physiology, 1994, 267(2):208-214.

[22] 贾斌, 赵茹茜, 剡根强, 等.半胱胺对羊毛生长及皮肤中GH受体、IGF-1受体和IGF-1型受体基因表达的影响[J].南京农业大学学报, 2005, 28(1):80-84.

[23] EKBERG S L, CARLSSON B, CARLSSON H, et al.Plasma growth hormone pattern regulates epidermal growth factor(EGF)receptor messenger ribonucleic acid levels and EGF binding in the rat liver[J].Endocrinology, 1989, 125:2158-2166.

[24] WYNN P C, BROWN G, MOORE G P.Characterization and distribution of epidermal growth factor receptors in the skin and wool follicles of the sheep fetus during development[J].Domestic Animal Endocrinology, 1995, 12(3):269-281.

[25] 姚玉园, 刘海英, 袁缨, 等.IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响[J].沈阳农业大学学报, 2010, 41(2):165-169.

[26] BATES E J, HYND P I, PENN N M, et al.Serum-free culture of wool folliclec:effects of nutrients growth factors and hormones[J].British Journal of Dermatology, 1997, 137(4):498-505.

[27] 赵宗胜, 王根林, 李大全, 李桂芳.IGF-1和EGF对绵羊毛囊体外培养的影响[J].南京农业大学学报, 2006, 29(2):138-141.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References