Effects of Vitamin D3 on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls

LI Siming; TANG Yanqiang; GU Deping; XIAO Haihong; WEI Qipeng; OUYANG Linghua

doi:10.3969/j.issn.1006-267x.2014.01.026

2014 , Vol. 26 >Issue 1: 197 - 202

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.01.026

Feed Science and Technology

Effects of Vitamin D₃ on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls

LI Siming ,
TANG Yanqiang ,
GU Deping ,
XIAO Haihong ,
WEI Qipeng ,
OUYANG Linghua

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Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2013-07-22

Online published: 2013-12-24

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Abstract

The aim of this experiment was to research the effects of vitamin D₃ on the relative expression levels of interleukin 2 (IL-2) and interleukin 18 (IL-18) genes in tissues of Silky fowls. One hundred and twenty Silky fowls were randomly allotted to 5 treatments with 4 replicates in each treatment and 6 fowls per replicate. Fowls in control treatment were fed a basal diet, while those in experimental treatments were fed the basal diet supplemented with 800, 1 600, 3 200 and 6 400 IU/kg vitamin D₃, respectively. Fowls were fed for 6 weeks in cages. The tissues (thymus, duodenum, jejunum, ileum, cecum and bursa of Fabricius) were collected for the analysis of relative expression levels of IL-2 and IL-18 genes. The results showed as follows: 6 400 IU/kg vitamin D₃ up-regulated the relative expression level of IL-2 gene in tissues of duodenum, bursa of Fabricius and cecum, but no regulation was found in tissues of thymus, jejunum and ileum; 6 400 IU/kg vitamin D₃ up-regulated the relative expression level of IL-18 gene in tissues of thymus, ileum, cecum and bursa of Fabricius of Silky fowls. The results indicate that dietary supplemental level of vitamin D₃ can affect the relative expression levels of IL-2 and IL-18 genes in Silky fowls, but the effects are differ in different tissues.

Key words： Silky fowl; vitamin D₃; interleukin

Cite this article

LI Siming , TANG Yanqiang , GU Deping , XIAO Haihong , WEI Qipeng , OUYANG Linghua . Effects of Vitamin D₃ on the Relative Expression Levels of Interleukin 2 and Interleukin 18 Genes in Tissues of Silky Fowls[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 197 -202 . DOI: 10.3969/j.issn.1006-267x.2014.01.026

References

[1] BONJOUR J P, CHEVALLEY T, FARDELLONE P.Calcium intake and vitamin D metabolism and action, in healthy conditions and in prostate cancer[J].The British Journal of Nutrition, 2007, 97(4):611-616.

[2] BIKLE D.Nonclassic actions of vitamin D[J].The Journal of Clinical Endocrinology and Metabolism, 2009, 94(1):26-34.

[3] 李思明, 欧阳玲花, 韦启鹏, 等.维生素D₃的免疫功能及其对家禽免疫细胞和免疫因子的调节[J].动物营养学报, 2012, 24(7):1189-1192.

[4] 李思明, 周定刚.维生素D₃对丝毛乌鸡的3个β-防御素基因在不同组织中表达的影响[J].营养学报, 2010, 22(1):93-94, 96.

[5] JONES G.Phosphorus metabolism and management in chronic kidney disease:expanding role for vitamin D in chronic kidney disease:importance of blood 25-OH-D levels and extra-renal 1α-hydroxylase in the classical and nonclassical actions of 1α, 25-dihydroxyvitamin D₃[J].Seminars in Dialysis, 2007, 20(4):316-324.

[6] 李思明, 周定刚.维生素D₃对丝毛乌骨鸡组织中β-防御素基因的表达调控[J].动物营养学报, 2009, 21(4):573-579.

[7] LI S M, OUYANG L H, ZHOU D G.Effects of vitamin D₃ on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky fowl[J].Czech Journal of Animal Science, 58(1):1-7.

[8] O'KELLY J, HISATAKE J, HISATAKE Y, et al.Normal myelopoiesis but abnormal T lymphocyte responses in vitamin D receptor knockout mice[J].The Journal of Clinical Investigation, 2002, 109(8):1091-1099.

[9] FROICU M, CANTORNA M T.Vitamin D and the vitamin D receptor are critical for control of the innate immune response to colonic injury[J].BMC Immunology, 2007, 8:5.

[10] KONG J, GRANDO S A, LI Y C.Regulation of IL-1 family cytokines IL-1alpha, IL-1 receptor antagonist, and IL-18 by 1, 25-dihydroxyvitamin D₃ in primary keratinocytes[J].Journal of Immunology, 2006, 176(6):3780-3787.

[11] TSE A K W, WAN C K, SHEN X L, et al.1, 25-dihydroxyvitamin D₃ induces biphasic NF-κB responses during HL-60 leukemia cells differentiation through protein induction and PI3K/Akt-dependent phosphorylation/degradation of IκB[J].Experimental Cell Research, 2007, 313(8):1722-1734.

[12] JAMES S Y, WILLIAMS M A, NEWLAND A C, et al.Leukemia cell differentiation:cellular and molecular interactions of retinoids and vitamin D[J].General Pharmacology:The Vascular System, 1999, 32(1):143-154.

[13] SZELES L, KERESZTES G, TOROCSIK D, et al.1, 25-dihydroxyvitamin D₃ is an autonomous regulator of the transcriptional changes leading to a tolerogenic dendritic cell phenotype[J].Journal of Immunology, 2009, 182(4):2074-2083.

[14] GRIFFIN M D, DONG X Y, KUMAR R.Vitamin D receptor-mediated suppression of RelB in antigen presenting cells:a paradigm for ligand-augmented negative transcriptional regulation[J].Archives of Biochemistry and Biophysics, 2007, 460(2):218-226.

[15] MATHIEU C, VAN ETTEN E, DECALLONNE B, et al.Vitamin D and 1, 25-dihydroxyvitamin D₃ as modulators in the immune system[J].The Journal of Steroid Biochemistry and Molecular Biology, 2004, 89/90(1/2/3/4/5):449-452.

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