Short Communications

Plant Polysaccharides:Effects on Lymphocytes Proliferation and Cytokines Secretion of Weaner Piglets

  • ZOU Yun ,
  • XIE Hongbing ,
  • YU Qifang ,
  • LI Jiangchang ,
  • HE Shaoping ,
  • HE Jianhua
Expand
  • College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2013-08-22

  Online published: 2013-12-24

Abstract

A 2×2×2 factorial design trial was conducted to investigate the effects and interactions of Achyranthes bidentata polysaccharides (ABP), Atractylodes macrocephalaon polysaccharides (AMP) and Astragalus polysaccharides (AP) on lymphocytes proliferation and cytokines secretion of weaner piglets, and to explore the immunity modulation mechanism of plant polysaccharides. A total of two hundred and fifty six 28-day-old Duroc×Landrace×Yorkshire crossbred weaner piglets were randomly divided into 8 treatments and each treatment had 4 replicates with 8 piglets per replicate. On the 14th and 28th day, one piglet of each replicate was randomly picked out for blood collection from thorax cavity vein for the determination of lymphocytes proliferation in peripheral blood and serum cytokines interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factors (TNF) and interferon-γ (IFN-γ) contents. The results showed as follows: 1) the addition of plant polysaccharides significantly increased lymphocytes proliferation in peripheral blood of weaner piglets (P<0.05). Adding ABP, AMP and AP at the same time increased lymphocyte proliferation in comparison with using only one (P<0.05) and two of them (P>0.05). 2) Compare with control group, serum IL-2, IL-1β, TNF and IFN-γ contents of weaner piglets fed the diet with ABP, AMP or AP were significantly increased (P<0.05), thought IL-6 content was increased in some extent, but there were no significant differences between the control group and the groups adding ABP, AMP or AP alone (P>0.05). Adding the two of ABP, AMP and AP or all of them together significantly improved serum IL-2, IL-1β, IL-6, IFN-γ and TNF contents compared with control group (P<0.05). It is concluded that the addition of ABP, AMP and AP alone or together in the diet can improve lymphocytes proliferation in peripheral blood and increase serum cytokines IL-2, IL-1β, IL-6,TNF and IFN-γ contents of wearer piglets.

Cite this article

ZOU Yun , XIE Hongbing , YU Qifang , LI Jiangchang , HE Shaoping , HE Jianhua . Plant Polysaccharides:Effects on Lymphocytes Proliferation and Cytokines Secretion of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 210 -218 . DOI: 10.3969/j.issn.1006-267x.2014.01.028

References

[1] LEUNG M Y K, LIU C, KOON J C M, et al.Polysaccharides biologic response modifiers[J].Immunology Letters, 2006, 105(2):101-114.  

[2] 陈清华, 刘祝英, 贺建华.牛膝多糖对仔猪淋巴细胞增殖作用和细胞因子分泌量的影响[J].动物营养学报, 2008, 20(6):712-717.

[3] 朱南山, 张彬, 李丽立, 等.白术多糖对仔猪血淋巴细胞转化及信号转导相关分子的影响[J].华北农学报, 2007, 22(2):18-21.

[4] LIU Q Y, YAO Y M, ZHANG S W, et al.Astragalus polysaccharides regulate T cell-mediated immunity via CD11chigh CD45RBlow DCs in vitro[J].Journal of Ethnopharmacology, 2011, 136(3):457-464.  

[5] 何昭阳, 胡桂学, 王春风.动物免疫学实验技术.长春:吉林科学技术出版社, 2002:156-157.

[6] KIM H M, HAN S B, OH G T, et al.Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus[J].International Journal of Immunopharmacology, 1996, 18(5):295-303.  

[7] 李宗锴, 李电东.牛膝多糖的免疫调节作用[J].药学学报, 1997, 32(12):881-887.

[8] 李丽立, 张彬, 朱南山, 等.多糖对早期断奶仔猪淋巴细胞增殖与细胞因子的影响[J].农业现代化研究, 2009, 30(4):495-497.

[9] 陈洪亮, 糕宝山, 李德发, 等.黄芪多糖对肉鸡脾淋巴细胞转化及信息分子的影响[J].中国兽医杂志, 2003, 39(10):11-14.

[10] 毛晓峰.黄芪多糖影响断奶仔猪免疫功能及其作用机理的研究[D].硕士学位论文.北京:中国农业大学, 2004:1-77.

[11] 范云鹏, 王德云, 胡元亮, 等.黄芪多糖脂质体对鸡淋巴细胞增殖的影响[J].南京农业大学学报, 2011, 34(4):95-99.

[12] LI L L, YIN F G, ZHANG B, et al.Dietary supplementation with Atractylodes macrophala Koidz polysaccharides ameliorate metabolic status and improve immune function in early-weaned pigs[J].Livestock Science, 2011, 142(1/2/3):33-41.

[13] ZHUGE Z Y, ZHU Y H, LIU P Q, et al.Effects of Astragalus polysaccharide on immune responses of porcine PBMC stimulated with PRRSV or CSFV[J].PloS One, 2012, 7(1):e29320.

[14] FAN Y P, HU Y L, WANG D Y, et al.Effects of Astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo[J].Carbohydrate Polymers, 2012, 88(1):68-74.  

[15] LI R, CHEN W C, WANG W P, et al.Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer[J].Carbohydrate Polymers, 2009, 7(4):738-742.

[16] QIU H H, CHENG G L, XU J Q, et al.Effects of Astragalus polysaccharides on associated immune cells and cytokines in immunosuppressive dogs[J].Procedia in Vaccinology, 2010, 2(1):26-33.  

[17] ZHU X T, PAN Y Y, ZHENG L, et al.Polysaccharides from the Chinese medicinal herb Achyranthes bidentata enhance anti-malarial immunity during Plasmodium yoelⅡ 17XL infection in mice[J].Malaria Journal, 2012, 11(1):49.

[18] JIN L Q, ZHENG Z J, PENG Y, et al.Opposite effects on tumor growth depending on dose of Achyranthes bidentata polysaccharides in C57BL/6 mice[J].International Immunopharmacology, 2007, 7(5):568-577.  

[19] GUO G L, LIU Y L, FAN W, et al.Effects of Achyranthes bidentata polysaccharide on growth performance, immunological, adrenal, and somatotropic responses of weaned pigs challenged with Escherichia coli lipopolysaccharide[J].Asian-Australasian Journal of Animal Science, 2008, 21(8):1189-1195.

[20] CHEN Q H, LIU Z Y, HE J H, et al.Achyranthes bidentata polysaccharide enhances growth performance and health status in weaned piglets[J].Food and Agricultural Immunology, 2011, 22(1):17-29.  
Outlines

/