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Effects of Bacillus subtilis var. natto B4 on Activity, Adhesion and Cytokine Secretion of Caco-2 Cells

  • SHANG Qinqin ,
  • HUANG Qin ,
  • XU Xin ,
  • SHI Yanyun ,
  • LI Weifen ,
  • YU Dongyou
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  • Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, Institute of Feed Science, College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2013-07-15

  Online published: 2013-12-24

Abstract

Using Caco-2 cells as an in vitro model to investigate the effects of Bacillus subtilis var. natto B4 on the activity, adhesion and cytokine secretion of Caco-2 cells. The trypan blue dye exclusion test and extracellular lactate dehydrogenase activity determination were conducted to research the influence of Bacillus subtilis var. natto B4 on the activity of Caco-2 cells; the fluorescence labeling technology was used to investigate the adhesive properties of Escherichia coli K88 and Salmonella choleraesuis to Caco-2 cells. The competition, rejection and displacement experiments were carried out to determine the blocking action of Bacillus subtilis var. natto B4 to the adhesion of the two pathogenic bacterial strains; the cytokines secreted by Caco-2 cells were measured by ELISA. The results showed as follows: 1) Bacillus subtilis var. natto B4 did not significantly affect survival rate of Caco-2 cells (P>0.05) and had no cytotoxicity on Caco-2 cells. 2) Bacillus subtilis var. natto B4 had no significant effects on the adhesion rate of Escherichia coli K88 and Salmonella choleraesuis to Caco-2 cells (P>0.05), and had no blocking action on the adhesion of the two pathogenic bacterial strains to Caco-2 cells. 3) Bacillus subtilis var. natto B4 significantly increased the content of pro-inflammatory cytokine inducing ligand (P<0.01), and had no significant influence on the contents of interleukin-6 and interleukin-8 (P>0.05), whereas Bacillus subtilis var. natto B4 significantly reduced the content of anti-inflammatory cytokine interleukin-10 (P<0.01). In conclusion, Bacillus subtilis var. natto B4 has no cytotoxicity against Caco-2 cells, and can modulate the cytokine secretion function of Caco-2 cells to induce some immune responses rather than block the adhesion of Escherichia coli K88 and Salmonella choleraesuis to Caco-2 cells.

Cite this article

SHANG Qinqin , HUANG Qin , XU Xin , SHI Yanyun , LI Weifen , YU Dongyou . Effects of Bacillus subtilis var. natto B4 on Activity, Adhesion and Cytokine Secretion of Caco-2 Cells[J]. Chinese Journal of Animal Nutrition, 2014 , 26(1) : 279 -286 . DOI: 10.3969/j.issn.1006-267x.2014.01.036

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