Effects of Supplemental Levels of Rumen-Protected Methionine and Lysine on Performance and Plasma Biochemical Indices of Lactating Holstein Cows

LIU Fei; LIN Xueyan; HOU Qiuling; WANG Yun; WANG Zhonghua

doi:10.3969/j.issn.1006-267x.2014.02.011

2014 , Vol. 26 >Issue 2: 363 - 370

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.011

Ruminant Nutrition

Effects of Supplemental Levels of Rumen-Protected Methionine and Lysine on Performance and Plasma Biochemical Indices of Lactating Holstein Cows

LIU Fei ,
LIN Xueyan ,
HOU Qiuling ,
WANG Yun ,
WANG Zhonghua

Expand

College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2013-08-27

Online published: 2014-01-27

Fold

Abstract

This experiment was conducted to investigate the effects of the supplementatl levels of rumen-protected methionine (RPMet) and lysine (RPLys) on performance and plasma biochemical indices of lactating Holstein cows under typical dietary condition in northern China. One hundred and sixty lactating Holstein cows in peak lactation period and with milk yield produced about 30 kg/d were selected and randomly allotted to four groups with 40 cows in each group. Cows in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 13 g/d RPMet+6 g/d RPLys (M13+L6 group), 43 g/d RPMet+98 g/d RPLys (M43+L98 group), and 74 g/d RPMet+190 g/d RPLys (M74+L190 group),respectively. The experiment consisted of a 10-day pre-experimental period and a 50-day experimental period. The results showed as follows: 1) dietary supplementation of RPMet and RPLys had no significant effects on dry matter intake (P>0.05). 2) Milk yield, milk fat yield and lactose yield in M74+L190 group were significantly higher than those in control group and M13+L6 group (P<0.05), while milk urinary nitrogen concentration was significantly lower than that in control group and M13+L6 group (P<0.05). 3) Compared with control group, plasma lysine concentration in M74+L190 group was significantly increased (P<0.05), while plasma urinary nitrogen concentration was significantly decreased (P<0.05); plasma methionine and triglyceride concentrations in M43+L98 and M74+M190 groups were significantly increased (P<0.05). In conclusion, under the typical dietary condition of northern China, the supplementation of RPMet and RPLys can improve performance of lactating cows in peak locating period, and the suitable supplemental levels of RPMet and RPLys for cows produced about 30 kg/d milk are 74 and 190 g/d, respectively.

Key words： lactation; cows; rumen-protected methionine; rumen-protected lysine; milk yield; plasma biochemical indices

Cite this article

LIU Fei , LIN Xueyan , HOU Qiuling , WANG Yun , WANG Zhonghua . Effects of Supplemental Levels of Rumen-Protected Methionine and Lysine on Performance and Plasma Biochemical Indices of Lactating Holstein Cows[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 363 -370 . DOI: 10.3969/j.issn.1006-267x.2014.02.011

References

[1] 冯薇, 王加启, 卜登攀.奶牛过瘤胃蛋氨酸和过瘤胃赖氨酸营养研究进展[J].中国奶牛, 2009(12):17-21.

[2] KING K J, BERGEN W G, SNIFFEN C J, et al.Anassessment of absorbable lysine requirements in lactating cows[J].Journal of Dairy Science, 1991, 74:2530-2539.

[3] POLAN C E, CUMMINS K A, SNIFFEN C J, et al.Responses of dairy cows to supplemental rumen-protected forms of methionine and lysine[J].Journal of Dairy Science, 1991, 74(9):2997-3013.

[4] SCHWAB G C, BOZAK C K, WHITEHOUSE N L, et al.Amino acid limitation and flow to the duodenum at four stages of lactation Ⅰ sequence of lysine and methionine limitation[J].Journal of Dairy Science, 1992, 75:3486-3502.

[5] ARMENTANO L E, BERRICS S J, DUCHARME G A.Response of lactating cows to methionine or methionine plus lysine added to high protein diets based on alfalfa and heated soybeans[J].Journal of Dairy Science, 1997, 80:l194-1199.

[6] SCHINGOETHE D J, CASPER D P, YANG C, et al.Lactational response to soybean meal, heated soybean meal and extruded soybeans with ruminally protected methioine[J].Journal of Dairy Science, 1998, 71:173-180.

[7] NRC.Nutrient requirements of dairy cattle[M].7th ed.Washington, D.C.:National Academy Press, 2001.

[8] 冯仰廉, 陆治年.奶牛营养需要和饲料成分[M].北京:中国农业出版社, 2007.

[9] VARVIKKO T, VANHATALO A, JALAVA T, et al.Lactation and metabolic responses to graded abomasal doses of methionine and lysine in cows fed grass silage diets[J].Journal of Dairy Science, 1999, 82(12):2659-2673.

[10] BLUM J W, BRUCKMAIER R M, JANS F.Rumen-protected methionine fed to dairy cows: bioavailability and effects on plasma amino acid pattern and plasma metabolite and insulin concentrations[J].Journal of Dairy Science, 1999, 82(9):1991-1998.

[11] MADSEN T G, NIELSEN L, NIELSEN M O.Mammary nutrient uptake in response to dietary supplementation of rumen protected lysine and methionine in late and early lactating dairy goats[J].Small Ruminant Research, 2005, 56(1):151-164.

[12] SOCHA M T, PUTNAM D E, GARTHWAITE B D, et al.Improving intestinal amino acid supply of pre-and postpartum dairy cows with rumen-protected methionine and lysine[J]. Journal of Dairy Science, 2005, 88(3):1113-1126.

[13] WANG C, LIU H Y, WANG Y M, et al.Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows[J].Journal of Dairy Science, 2010, 93(8):3661-3670.

[14] 徐元年, 张幸开, 袁耀明, 等.过瘤胃赖氨酸和过瘤胃蛋氨酸对泌乳早期奶牛生产性能影响的研究[J].乳业科学与技术, 2007, 1:43-45.

[15] ROGERS J A, KRISHNAMOORTHY U, SNIFFEN C J.Plasma amino acids and milk protein production by cows fed rumen-protected methionine and lysine[J].Journal of Dairy Science, 1987, 70(4):789-798.

[16] SWANEPOEL N, ROBINSON P H, ERASMUS L J.Amino acid needs of lactating dairy cows:Impact of feeding lysine in a ruminally protected form on productivity of lactating dairy cows[J].Animal Feed Science and Technology, 2010, 157(1):79-94.

[17] YANG W R, SUN H, WANG Q Y, et al.Effects of rumen-protected methionine on dairy performance and amino acid metabolism in lactating cows[J].American Journal of Animal and Veterinary Sciences, 2010, 5(1):1-7.

[18] WATANABE K, FREDEEN A H, ROBINSON P H, et al.Effects of fat coated rumen bypass lysine and methionine on performance of dairy cows fed a diet deficient in lysine and methionine[J].Animal Science Journal, 2006, 77(5):495-502.

[19] APPUHAMY J A D R N, KNAPP J R, BECVAR O, et al.Effects of jugular-infused lysine, methionine and branched-chain amino acids on milk protein synthesis in high-producing dairy cows[J].Journal of Dairy Science, 2011, 94(4):1952-1960.

[20] 孙海洲, 沈美英, 卢德勋, 等.乳中尿素氮在奶牛营养检测中的应用[J].畜牧与饲料科学, 2005, 2:47-47.

[21] OVERTON T R, EMMERT L S, CLARK J H.Effects of source of carbohydrate and protein and rumen-protected methionine on performance of cows[J].Journal of Dairy Science, 1998, 81(1):221-228.

[22] 王洪荣, 卢德勋.饲喂玉米型饲粮的生长绵羊限制性氨基酸研究[J].动物营养学报, 1999, 11(4):17-28.

[23] 孙华, 张晓明, 王欣, 等.过瘤胃保护蛋氨酸对奶牛乳和血浆氨基酸含量及血液生化指标的影响[J].中国奶牛, 2010, (012):7-11.

[24] 吴志广.饲粮补饲蛋氨酸锌对奶牛生产性能及血液生化指标的影响[J].硕士学位论文.保定:河北农业大学, 2006.

[25] RULQUIN H, DELABY.Effects of the energy balance of dairy cows in lactational responses to rumen-protected methionine[J].Journal of Dairy Science, 1997, 80:2513-2522.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References