Response of Dietary Restriction of Oat Hay on Ruminal Fermentation Parameters of Tibetan Sheep and Estimation of Maintenance Requirement of Nitrogen

ZHANG Ying; ZHOU Jianwei; LIU Hao; MI Jiandui; LONG Ruijun

doi:10.3969/j.issn.1006-267x.2014.02.012

2014 , Vol. 26 >Issue 2: 371 - 379

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.012

Ruminant Nutrition

Response of Dietary Restriction of Oat Hay on Ruminal Fermentation Parameters of Tibetan Sheep and Estimation of Maintenance Requirement of Nitrogen

ZHANG Ying ,
ZHOU Jianwei ,
LIU Hao ,
MI Jiandui ,
LONG Ruijun

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1. College of Pastoral Agriculture Science and Technology of Lanzhou University, Lanzhou 730000, China;
2. International Centre for Tibetan Plateau Ecosystem Management of Lanzhou University, Lanzhou 730000, China;
3. School of Life Science of Lanzhou University, Lanzhou 730000, China

Received date: 2013-08-20

Online published: 2014-01-27

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Abstract

The objective of this study was to evaluate the effects of dietary restriction of oat hay on ruminal fermentation parameters of Tibetan sheep, and to estimate maintenance requirement of nitrogen. Five 20-month-old castrated male Tibetan sheep with similar body weight [(45±2) kg] were selected in a feeding trial and a fasting metabolism trial. Five sheep were randomly divided into four groups (two sheep in one of the groups as replicate) in the feeding trial using a 4 × 4 Latin square design. Four dietary restriction levels were set, which were 90%, 70%, 50 and 30% of voluntary intake, respectively. Feces, urine and rumen fluid (after 0, 2,4, 6 and 8 hours of feeding) were collected to measure the indices of digestion and metabolism of nutrients and rumen fermentation metabolic parameters. Feces and urine were collected to measure daily output of fecal and urinary nitrogen in the fasting metabolism trial. The results showed as follows: 1) dietary restriction level had no significant effects on rumen fluid pH, the contents of acetate, propionate, isobutyrate, valerate and isovalerate, and acetate/propionate (P>0.050), but had significant effects on butyrate content and total volatile fatty acid concentration (P<0.050 or P<0.001). 2) Dietary restriction level had no significant effects on the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) (P>0.050). 3) With the increase of fasting time, fecal nitrogen showed a tendency of being firstly decreased and then balanced, and urinary nitrogen and total nitrogen output both showed a tendency of being firstly decreased and then balanced. In conclusion, dietary restoration level does not affect nutrient digestibility of Tibetan sheep. Nitrogen maintenance requirement during regular feeding period is 0.12 g/(kg BW^0.75·d), and that during fasting period is 0.15 g/(kg BW^0.75·d).

Key words： Tibetan sheep; rumen fermentation; nitrogen metabolism; maintenance requirement

Cite this article

ZHANG Ying , ZHOU Jianwei , LIU Hao , MI Jiandui , LONG Ruijun . Response of Dietary Restriction of Oat Hay on Ruminal Fermentation Parameters of Tibetan Sheep and Estimation of Maintenance Requirement of Nitrogen[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 371 -379 . DOI: 10.3969/j.issn.1006-267x.2014.02.012

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