Comparison of Effects of Crystalline Tryptophane and Coated Tryptophane on Growth Performance, Protein Metabolism and Abilities of Digestion and Absorption of Juvenile Jian Carp (Cyprinus carpio var. Jian)

TANG Ling; SUN Chongyan; KUANG Shengyao; FENG Lin; ZHOU Xiaoqiu

doi:10.3969/j.issn.1006-267x.2014.02.017

2014 , Vol. 26 >Issue 2: 411 - 419

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.017

Aquaculture Nutrition

Comparison of Effects of Crystalline Tryptophane and Coated Tryptophane on Growth Performance, Protein Metabolism and Abilities of Digestion and Absorption of Juvenile Jian Carp (Cyprinus carpio var. Jian)

TANG Ling ,
SUN Chongyan ,
KUANG Shengyao ,
FENG Lin ,
ZHOU Xiaoqiu

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1. Animal Nutrition Institute of Sichuan Agriculture University, Wenjiang 611130, China;
2. Animal Nutrition Institute of Sichuan Animal Science Institute, Chengdu 610099, China;
3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Ya'an 625014, China

Received date: 2013-08-07

Online published: 2014-01-27

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Abstract

This experiment was conducted to compare the effects of crystalline tryptophane and coated tryptophane on growth performance, protein metabolism and the abilities of digestion and absorption of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 300 juvenile Jian carp with an initial body weight of (7.73±0.03) g were randomly divided into 2 groups (with 3 replicates per group and 50 fish per replicate), and the fish in the 2 groups had been fed experimental diets containing crystalline tryptophane and coated tryptophane for 60 days, respectively. The tryptophane content was 0.25% both for 2 experimental diets. The results showed as follows: the specific growth rate, protein efficiency ratio, protein retention rate and lipid retention rate in coated tryptophane group were significantly higher and the feed conversion ratio in coated tryptophane group was significantly lower than those in crystalline tryptophane group (P<0.05). Compared with the crystalline tryptophane, the coated tryptophane significantly improved the intestosomatic index, intestinal length index, hepatosomatic index, intestinal protein content, hepatopancreas protein content and the fold heights of anterior intestine, middle intestine and posterior intestine (P<0.05); significantly increased the activities of trypsin and lipase in intestine and hepatopancreas (P<0.05); significantly increased the activities of Na⁺,K⁺-ATPase and γ-glutamyl transpeptidase (γ-GT) in middle intestine and posterior intestine and alkaline phosphatase (AKP) in anterior intestine, middle intestine and posterior intestine (P<0.05). Moreover, the concentration of plasma ammonia and the activities of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) in hepatopancreas and muscle in coated tryptophane group were significantly lower than those in crystalline tryptophane group (P<0.05). The results of release rate of tryptophan in vitro showed that crystalline tryptophane and coated tryptophane were all released at 15 and 180 min, respectively. In conclusion, compared with the crystalline tryptophane, the coated tryptophane has a lower release rate; therefore, it can improve the growth performance and the abilities of digestion and absorption more effectively, which is beneficial to protein synthesis of juvenile Jian carp.

Key words： Cyprinus carpio var. Jian; crystalline tryptophan; coated tryptophan; growth; digestive enzyme; brush border enzyme

Cite this article

TANG Ling , SUN Chongyan , KUANG Shengyao , FENG Lin , ZHOU Xiaoqiu . Comparison of Effects of Crystalline Tryptophane and Coated Tryptophane on Growth Performance, Protein Metabolism and Abilities of Digestion and Absorption of Juvenile Jian Carp (Cyprinus carpio var. Jian)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 411 -419 . DOI: 10.3969/j.issn.1006-267x.2014.02.017

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