Effects of Pectinase in Meal Type Diets on Ileal Amino Acid Digestibility and Metabolizable Energy of Broilers

LEI Ting; GUO Yuming; WANG Yaohui; NING Dong; LUO Xuan

doi:10.3969/j.issn.1006-267x.2014.02.022

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 2: 453 - 465

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.022

Feed Science and Technology

Effects of Pectinase in Meal Type Diets on Ileal Amino Acid Digestibility and Metabolizable Energy of Broilers

LEI Ting ,
GUO Yuming ,
WANG Yaohui ,
NING Dong ,
LUO Xuan

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1. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Asiapac Biotechnology Co., Ltd., Dongguan 523808, China

Received date: 2013-09-13

Online published: 2014-01-27

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Abstract

This study was conducted to investigate the effects of pectinase in meal type diets on ileal amino acid digestibility and metabolizable energy of broilers. A total of 780 one-day-old male Ross 308 broilers were randomly divided into 13 treatments (6 replicates per treatment and 10 broilers per replicate), the 12 treatments of them were fed soybean meal, cottonseed meal and rapeseed meal type semi-purified diets, respectively, which were all with pectinase group and control group. Another one treatment was fed a nitrogen-free diet to determine endogenous amino acid losses. Total feces of broilers were collected from 20 to 22 days of age to determine the effects of pectinase on the metabolizable energy of broilers, and the terminal half of ileal digesta was collected at 23 days of age to determine the effects of pectinase on amino acid digestibility of broilers. The results showed as follows: 1) the apparent metabolizable energy (AME) and apparent metabolizable energy corrected for nitrogen (AMEn) of the high quality soybean meal were significantly higher than those of normal soybean meal (P<0.01), the AME and AMEn of three kinds of cottonseed meals had significant differences (P<0.05), and the values of them from high to low were cottonseed protein, dephenol cottonseed meal and general cottonseed meal. After adding pectinase, the AME and AMEn of two kinds of soybean meals had a tend of increase (P=0.066 and P=0.064, respectively), and the AME and AMEn of three kinds of cottonseed meals were significantly increased (P<0.01), while the AME and AMEn of rapeseed meal had no significant change (P>0.05). 2) There was no significant difference on standardized ileal digestibility (SID) of most amino acids between two kinds of soybean meals except phenylalanine, tyrosine and aspartic acid (P>0.05), and the SID of all amino acids was significantly different between three kinds of cottonseed meals (P<0.05). The SID of the methionine and proline of two kinds of soybean meals was increased by pectinase supplementation (P=0.087 and P=0.082, respectively). The SID of some amino acids of three kinds of cottonseed meals showed the interaction effect between pectinase and cottonseed meal (P<0.05). The SID of most amino acids of rapeseed meal was increased by pectinase supplementation (P>0.05). In conclusion, the AME, AME_n and amino acid SID of the same ingredient with different processes are different, and the supplementation of pectinase can increase the metabolizable energy and amino acid digestibility except rapeseed meal.

Key words： broiler; pectinase; metabolizable energy; ileal; amino acid digestibility

Cite this article

LEI Ting , GUO Yuming , WANG Yaohui , NING Dong , LUO Xuan . Effects of Pectinase in Meal Type Diets on Ileal Amino Acid Digestibility and Metabolizable Energy of Broilers[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 453 -465 . DOI: 10.3969/j.issn.1006-267x.2014.02.022

References

[1] 刘文, 董赛丽, 梁金亚.果胶的性质、功能及其应用[J].三门峡职业技术学院学报, 2008, 7(2):118-121.

[2] 陈喜斌.饲料学[M].北京:科学出版社, 2003:12-13.

[3] 周小乔, 王宝维.饲用果胶酶研究的进展[J].饲料研究, 2010(8):14-16.

[4] SHORT F J, GORTON P, WISEMAN J, et al.Determination of titanium dioxide added as an inert marker in chicken digestibility studies[J].Animal Feed Science and Technology, 1996, 59(4):215-221.

[5] 邓雪娟, 刘国华, 蔡辉益, 等.分光光度计法测定家禽饲料和食糜中二氧化钛[J].饲料工业, 2008, 29(2):57-58.

[6] 王永伟, 刘国华.家禽饲料有效能值的测定及影响因素[J].饲料工业, 2009, 30(11):41-44.

[7] 张春雷, 侯水生, 刘福柱, 等.影响家禽饲料代谢能的因素[J].饲料工业, 2003, 24(8):11-13.

[8] MATEOS G G, SELL J L.Influence of fat and carbohydrate source on rate of food passage of semipurified diets for laying hens[J].Poultry Science, 1981, 60(9):2114-2119.

[9] KETELS E, DE GROOTE G.Effect of ratio of unsaturated to saturated fatty acids of the dietary lipid fraction on utilization and metabolizable energy of added fats in young chicks[J].Poultry Science, 1989, 68(11):1506-1512.

[10] VILLAMIDE M J, SAN JUAN L D.Effect of chemical composition of sunflower seed meal on its true metabolizable energy and amino acid digestibility[J].Poultry Science, 1998, 77(12):1884-1892.

[11] 郑萍, 李波, 陈代文, 等.8种不同来源菜籽饼粕的生长猪氨基酸回肠消化率评定[J].中国畜牧杂志, 2012, 48(9):34-40.

[12] 席鹏彬, 马永喜, 李德发, 等.中国菜籽饼粕化学组成特点及其影响因素的研究[J].中国畜牧杂志, 2004, 40(10):12-15.

[13] 艾晓杰.家禽日粮抗营养因子的危害及对策[J].中国家禽, 2004, 26(1):8-11.

[14] 龙玲, 吴达.饲料抗营养因子概述[J].饲料博览, 2001(7):12-16.

[15] 王冲, 娄玉杰.常见饲料中抗营养因子及对动物的影响[J].家畜生态, 2000, 21(4):39-43.

[16] 常磊.棉粕固态发酵及游离棉酚降解的研究[D].硕士学位论文.广州:暨南大学, 2012.

[17] 荆丽珍, 王宝维, 魏笑笑, 等.绿色饲料添加剂——果胶酶的研究[J].饲料工业, 2007, 28(14):6-8.

[18] 薛长湖, 张永勤, 李兆杰, 等.果胶及果胶酶研究进展[J].食品与生物技术学报, 2005, 24(6):94-99.

[19] SCHNEEMAN B O.Soluble vs insoluble fiber:different physiological responses[J].Food Technology, 1987, 41(2):81-82.

[20] ROBERFROID M.Dietary fiber, inulin, and oligofructose:a review comparing their physiological effects[J].Critical Reviews in Food Science & Nutrition, 1993, 33(2):103-148.

[21] CHOCT M, ANNISON G.Anti-nutritive effect of wheat pentosans in broiler chickens:roles of viscosity and gut microflora[J].British Poultry Science, 1992, 33(4):821-834.

[22] LAGRECA L, MAROTTA E.Nutritional effect of pectin in swine during growth and before slaughter[J].Arch Latinoam Nutrion, 1985, 35(1):172-179.

[23] LANGHOUT D J, SCHUTTE J B, DE JONG J, et al.Effect of viscosity on digestion of nutrients in conventional and germ-free chicks[J].British Journal of Nutrition, 2000, 83(5):533-540.

[24] LIN P H, SHIH B L, HSU J C.Effects of different sources of dietary non-starch polysaccharides on the growth performance, development of digestive tract and activities of pancreatic enzymes in goslings[J].British Poultry Science, 2010, 51(2):270-277.

[25] COWAN W D, PETTERSSONI D R, RASMUSSEN P B.The influence of multi-component pectinase enzymes on energy and amino acid availability in vegetable proteins[C]//Proceedings of the Australian Poultry Science Symposium. Sydney:University of Sydney, 1999, 11:85-88.

[26] 姜晓霞, 王宝维, 孙鹏, 等.鹅源草酸青霉产果胶酶对肉鸡生长发育影响研究[C]//中国家禽科学研究进展——第十四次全国家禽科学学术讨论会论文集.北京:中国畜牧兽医学会, 2009:737-743.

[27] 许毅.果胶酶对肉鸡玉米豆粕型日粮养分利用率的影响[D].硕士学位论文.南京:南京农业大学, 2004.

[28] 王春林, 陆文清, 李德发, 等.果胶酶对肉仔鸡生长性能及屠体性状的影响[J].中国畜牧杂志, 2004, 40(2):19-21.

[29] 方华平, 程茂基.果胶酶在食品与饲料工业中的应用[J].饲料博览, 2006(5):34-35.

[30] CHOCT M, HUGHES R J, WANG J, et al.Increased small intestinal fermentation is partly responsible for the anti-nutritive activity of non-starch polysaccharides in chickens[J].British Poultry Science, 1996, 37(3):609-621.

[31] MALATHI V, DEVEGOWDA G.In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes[J].Poultry Science, 2001, 80(3):302-305.

[32] 王宝维, 姜晓霞, 孙鹏, 等.鹅源草酸青霉产果胶酶对肉鸡消化生理影响的研究[J].动物营养学报, 2010, 22(2):358-364.

[33] MENG X, SLOMINSKI B A.Nutritive values of corn, soybean meal, canola meal, and peas for broiler chickens as affected by a multicarbohydrase preparation of cell wall degrading enzymes[J].Poultry Science, 2005, 84(8):1242-1251.

[34] KOCHER A, CHOCT M, PORTER M D, et al.The effects of enzyme addition to broiler diets containing high concentrations of canola or sunflower meal[J].Poultry Science, 2000, 79(12):1767-1774.

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