Molecular Nutrition

Analysis of Intestinal Bacterial Flora of Hylan Brown Laying Chicken Fed in Cage by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis

  • WANG Qiuju ,
  • CUI Yizhe ,
  • LIU Shengjun ,
  • YIN Hanzhou
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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2013-09-03

  Online published: 2014-01-27

Abstract

This study aimed to analyze the intestinal bacterial flora of Hylan brown laying chicken fed in cage by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Five 8-week-old chickens fed in cage were randomly selected and slaughtered to collect whole intestinal samples. Intestinal contents in duodenum, jejunum, ileum and cecum were individually mixed, and genome DNA of them were extracted, then sequences in hypervariable region of 16S rDNA were amplified with bacterial common primers, finally, the PCR products were used in DGGE analysis. The results showed as follows: there was obvious difference in bacterial flora composition among different intestinal segments. Eight, twelve, eight and nine strains of bacteria were isolated from duodenum, jejunum, ileum and cecum, respectively, twenty strains in all, and seven strains of Lactobacillus among those (including two strains of Lactobacillus aviaries, two strains of Lactococcus raffinolactis, one strain of Lactobacillus agilis, one strain of Coprococcus comes and one strain of Lactobacillus equigenerosi. There were 3 common strains of bacteria in all intestinal segments, which were one strain of RummelⅡbacillus stabekisⅡ, one strain of Lactobacillus equigenerosi and one strain of Gardnerella vaginalis. The results indicate that bacterial flora structure is relative with intestinal segment of Hylan brown laying chicken fed in cage, and there are differences in intestinal bacterial flora composition among different intestinal segments.

Cite this article

WANG Qiuju , CUI Yizhe , LIU Shengjun , YIN Hanzhou . Analysis of Intestinal Bacterial Flora of Hylan Brown Laying Chicken Fed in Cage by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 504 -512 . DOI: 10.3969/j.issn.1006-267x.2014.02.028

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