Effects of Glutamine on Cecal Mucosal Morphology and Inflammatory Cytokine mRNA Expressions of Dairy Goats Fed a High Concentrate Diet

LIU Yujie; LIU Junhua; XU Tingting; MAO Shengyong

doi:10.3969/j.issn.1006-267x.2014.02.029

2014 , Vol. 26 >Issue 2: 513 - 518

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.029

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Effects of Glutamine on Cecal Mucosal Morphology and Inflammatory Cytokine mRNA Expressions of Dairy Goats Fed a High Concentrate Diet

LIU Yujie ,
LIU Junhua ,
XU Tingting ,
MAO Shengyong

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College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2013-09-02

Online published: 2014-01-27

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Abstract

The objective of the present study was to investigate the effects of glutamine (Gln) on cecal mucosa morphology and inflammatory cytokine mRNA expressions of dairy goats fed a high concentrate diet. Twelve healthy dairy goats were randomly assigned to control group (n=6, fed a high concentrate diet) and Gln group (n=6, supplemental level of Gln was 50 g/d). After a 28-day experimental period, four goats in each group were slaughtered to collect cecal mucosa. The changes of tissue morphology of cecal mucosa, the mRNA expression levels of inflammatory cytokines in cecal mucosa and the concentrations of volatile fatty acids in cecal contents were measured. The results showed as follows: Gln supplementation did not affect the concentrations of volatile fatty acids, lactic acid and endotoxin contents in cecal (P>0.05); the supplementation of Gln played roles in keeping the integrity and enhancing tight junction of cecal mucosa; the supplementation of Gln significantly decreased the mRNA expression level of tumor necrosis factor α (P<0.05), but had no significant effects on that of interferon γ,interleukin 1β, interleukin 6 and interleukin 10 in cecal mucosa (P>0.05). It is suggested that Gln supplementation can enhance cecal mucosa tight junctions release cecal mucosal inflammatory response, to a certain extent, which is benefit for maintenance of mucosal barrier function of hindgut under the condition of the high concentrate diet.

Key words： glutamine; cecal mucosa; dairy goat; inflammatory cytokine

Cite this article

LIU Yujie , LIU Junhua , XU Tingting , MAO Shengyong . Effects of Glutamine on Cecal Mucosal Morphology and Inflammatory Cytokine mRNA Expressions of Dairy Goats Fed a High Concentrate Diet[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 513 -518 . DOI: 10.3969/j.issn.1006-267x.2014.02.029

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