Effects of Fructose and Insulin on Stearyl-Coenzyme A Desaturase 1 Gene Expression Level in Primary Germinal Hepatocytes of Geese

ZHANG Yihui; ZHANG Rui; ZHANG Jun; WANG Hongrong; GONG Daoqing

doi:10.3969/j.issn.1006-267x.2014.02.031

2014 , Vol. 26 >Issue 2: 526 - 532

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.031

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Effects of Fructose and Insulin on Stearyl-Coenzyme A Desaturase 1 Gene Expression Level in Primary Germinal Hepatocytes of Geese

ZHANG Yihui ,
ZHANG Rui ,
ZHANG Jun ,
WANG Hongrong ,
GONG Daoqing

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College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-09-02

Online published: 2014-01-27

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Abstract

The aim of this study was to detect the effects of fructose and insulin on stearyl-coenzyme A desaturase 1 (SCD1) gene expression level in primary germinal hepatocytes of geese. Primary germinal hepatocytes of geese were used as test object, the effects of different concentrations of fructose (0, 10, 30 and 50 mmol/L), insulin (0, 50, 100 and 200 nmol/L) and their combination (10 mmol/L fructose+50 nmol/L insulin, 10 mmol/L fructose+200 nmol/L insulin, 50 mmol/L fructose+50 nmol/L insulin and 50 mmol/L fructose+200 nmol/L insulin) on the SCD1 gene expression level in primary germinal hepatocytes of geese were detected. The results showed as follows: 10 mmol/L fructose could significantly up-regulate SCD1 gene expression level (P<0.01), and 30, 50 and 100 mmol/L fructose had no significant effect on it (P>0.05); 200 nmol/L insulin could significantly up-regulate SCD1 gene expression level (P<0.05); 10 mmol/L fructose and 200 nmol/L insulin had significantly synergistic effect on the up-regulation of SCD1 gene expression level. The results indicate that fructose and insulin alone supplementation and the combination at proper levels can up-regulate SCD1 gene expression level in primary germinal hepatocytes of geese.

Key words： fructose; insulin; primary germinal hepatocytes of geese; SCD1

Cite this article

ZHANG Yihui , ZHANG Rui , ZHANG Jun , WANG Hongrong , GONG Daoqing . Effects of Fructose and Insulin on Stearyl-Coenzyme A Desaturase 1 Gene Expression Level in Primary Germinal Hepatocytes of Geese[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 526 -532 . DOI: 10.3969/j.issn.1006-267x.2014.02.031

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