Short Communications

Comparative Analysis on the Capacities of Producing Nutricines for Six Yeasts

  • LU Chunfang ,
  • LIU Guojuan ,
  • LIU Dacheng ,
  • HU Honglian ,
  • GAO Min
Expand
  • 1. College of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China;
    3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2013-08-19

  Online published: 2014-01-27

Abstract

This experiment was conducted to find out the high activity strains for the development of ruminant microecological agents in latter. The capacities of producing nutricines (β-glucan, mannan, organic acids, amino acids and polypeptides) for 6 yeasts (Cadida atilis BY, Candida tropicalis BR, Candida tropicalis B2, Saccharomyces cerevisiae YR, Saccharomyces cerevisiae YC and Saccharomyces cerevisiae BC) were determined and the multi-indices synthetical analysis was used to analyze experimental data by Topsis method in DPS 14.10 software. The results showed as follows: the capacities of producing β-glucan and mannan of Candida tropicalis BR were the strongest, and the contents of β-glucan and mannan in cell wall of Candida tropicalis BR were 80.2 and 60.5 mg/g, respectively; the capacity of producing organic acids of Candida utilis BY was the strongest, and the content of total organic acids in culture of Candida utilis BY rearched 344.46 μg/mL; the capacity of producing polypeptides of Saccharomyces cerevisiae BC was the strongest, and the content of polypeptides was 14.08 mg/dL. Multi-indices synthetical analysis results showed that the optimal strain was Candida tropicalis BR, and it had the strongest capacities of producing β-glucan and mannan, succinic acid, glutamic acid and cysteine. The contents of β-glucan and mannan in cell wall of Candida tropicalis BR were 80.2 and 60.5 mg/g, respectively, and the contents of total organic acids and total anima acids in culture of Candida tropicalis BR were up to 312.11 μg/mL and 4.705 mg/g, respectively. The optimal strain with the highest capacities of producing nutricines is Candida tropicalis BR among 6 yeasts by comprehensive comparative analysis.

Cite this article

LU Chunfang , LIU Guojuan , LIU Dacheng , HU Honglian , GAO Min . Comparative Analysis on the Capacities of Producing Nutricines for Six Yeasts[J]. Chinese Journal of Animal Nutrition, 2014 , 26(2) : 533 -540 . DOI: 10.3969/j.issn.1006-267x.2014.02.032

References

[1] 帅丽芳, 段铭, 张光圣.微生态制剂对反刍动物消化系统的调控作用[J].中国饲料, 2002(9):16-17.

[2] PIVA G, BELLADONNA S, FUSCONI G, et al.Effects of yeast on dairy cow performance, ruminal fermentation, blood components, and milk manufacturing properties[J].Journal of Dairy Science, 1993, 76(9):2717-2722.  

[3] 唐海翠, 庞学东, 庄苏, 等.酵母培养物对山羊瘤胃纤维素酶活及挥发性脂肪酸的影响[J].中国畜牧杂志, 2006, 42(15):35-38.

[4] 王聪, 任金, 刘强, 等.酵母对奶牛泌乳性能及健康状况影响的研究[J].兽药与饲料添加剂, 2005, 10(1):7-9.

[5] 苑文珠, 刘建新, 吴月明.日粮中直接添加微生物制剂(DFM)对反刍动物的影响[J].饲料研究, 2001(2):1-3.

[6] SCHINGOETHE D J, LINKE K N, KALSCHEUR K F, et al.Feed efficiency of mid-lactation dairy cows fed yeast culture during summer[J].Journal of Dairy Science, 2004, 87(12):4178-4181.  

[7] LILA Z A, MOHAMMED N, YASUI T, et al.Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro[J].Journal of Animal Science, 2004, 82(6):1847-1854.

[8] CHAUCHERYRAS F, FONTY G, BERTIN G, et al.In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in association with an archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae[J].Applied and Environmental Microbiology, 1995, 61(9):3466-3467.

[9] 孙鸽.奶牛微生态制剂酵母菌优良特性的研究及其对瘤胃发酵的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学, 2012.

[10] 卢德勋.系统动物营养学导论[M].北京:中国农业出版社, 2004.

[11] CHAUCHEYRAS-DURAND F, WALKER N D, BACH A.Effects of active dry yeasts on the rumen microbial ecosystem:past, present and future[J].Animal Feed Science and Technology, 2008, 145(1/2/3/4):5-26.

[12] NEWBOLD C J, WALLACE R J, CHEN X B, et al.Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep[J].Animal Science, 1995, 73(6):1811-1818.

[13] DAWSON K A, HOPKINS D M.Differential effects of live yeast on the cellulolytic activities of anaerobic ruminal bacteria[J].Journal of Animal and Science, 1991, 69(Suppl.1):531.

[14] 刘晓永.酿酒酵母β-D-葡聚糖制备、构象及免疫功效研究[D].博士学位论文.无锡:江南大学, 2007:22-27.

[15] DALLIES N, FRANOIS J, PAQUET V.A new method for quantitative determination of polysaccharides in the yeast cell wall.Application to the cell wall defective mutants of Saccharomyces cerevisiae[J].Yeast, 1998, 14(14):1297-1306.  

[16] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 22221—2008食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖的测定高效液相色谱法[S].北京:中国标准出版社, 2008.

[17] 中华人民共和国卫生部, 中国国家标准化管理委员会.GB/T 5009.157—2003食品中有机酸的测定[S].北京:中国标准出版社, 2003.

[18] 中华人民共和国卫生部, 中国国家标准化管理委员会.GB/T 5009.124—2003食品中氨基酸的测定[S].北京:中国标准出版社, 2003.

[19] BOHLE B, ZWOLFER B, HERATIZADEH A, et al.Cooking birch pollenrelated food:divergent consequences for IgE-and T cellmediated reactivity in vitro and in vivo[J].Journal of Allergy and Clinical Immunology, 2006, 118(1):242-249.  

[20] 唐启义.DPS©数据处理系统:实验设计, 统计分析及数据挖掘[M].2版.北京:科学出版社, 2010.

[21] 李声永, 王加启, 龚月生, 等.酵母培养物在反刍动物日粮中的应用研究进展[J].中国畜牧兽医, 2002, 29(5):18-22.

[22] 卢德勋.国际动物营养学的发展趋势与我们对策的思考[C].北京:中国农业科学技术出版社, 2006:8-17.

[23] ADAMS C A.The role of nutricines in health and total nutrition[J].Proceedings of Australian Poultry Science Symposim, 2000, 12:17-24.

[24] JAWHARA S, MOGENSEN E, MAGGIOTTO F, et al.Murine model of dextran sulfate sodium-induced colitis reveals Candida glabrata virulence and contribution of β-mannosyltransferases[J].The Journal of Biological Chemistry, 2012, 287:11313-11324.

[25] LAMKANFI M, MALIREDDI R K, KANNEGANTI T D.Fungal zymosan and mannan activate the cryopyrin inflammasome[J].The Journal of Biological. Chemistry, 2009, 284:20574-20581.

[26] 孙海翔, 尹卓容, 马美范.高压均质破碎啤酒酵母细胞壁的研究[J].食品工业科技, 2002, 23(2):66-67.
Outlines

/