Swine Nutrition

Solation, Identification and Enzymatic Characteristics Analysis of Cellulolytic Bacterium from Tibetan Pigs

  • YANG Weiping ,
  • MENG Fanxu ,
  • MA Li ,
  • JI Shengyue ,
  • CAO Binyun
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  • 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
    2. Luoyang Normal University, Luoyang 471022, China

Received date: 2013-10-17

  Online published: 2014-03-04

Abstract

This experiment aimed to isolate, screen and identify a highly cellulose-decomposing bacterium isolated from the cecal contents of Tibetan pigs, and the enzymatic characteristics were also analysed. A cellulolytic bacterium which could highly decompose cellulose was screened by using carboxymethylcellulose (CMC) plate method (primary screening) and shake-flask fermentation method (secondary screening). Combined the morphological characteristics, physiological and biochemical characteristics and 16S rRNA gene sequence homology analysis, the bacterial stain was identified. Enzyme-producing ability of the bacterial strain was evaluated based on the growth characteristics and enzyme-producing characteristics at different times. Furthermore, the enzymatic characteristics of the bacterial strain were studied based on the suitable reaction pH, temperature and time of carboxymethyl cellulose (CMCase) produced by the bacterial strain. The results showed that a highly cellulose-decomposing bacterium was newly isolated from the cecal contents of Tibetan pigs and identified as Bacillus subtilis BY-2. The strain of Bacillus subtilis BY-2 was found to be a spore-forming, gram-positive and aerobic bacterium with efficient growth rate, earlier and longtime producing enzyme. The result of enzymatic characteristics analysis showed that the suitable reaction condition of CMCase produced by Bacillus subtilis BY-2 was pH 5.5 and 65 ℃ with a reaction time of 5 min. Besides, the CMCase had a stronger acidic and basic stability when pH range was 3 to 8.

Cite this article

YANG Weiping , MENG Fanxu , MA Li , JI Shengyue , CAO Binyun . Solation, Identification and Enzymatic Characteristics Analysis of Cellulolytic Bacterium from Tibetan Pigs[J]. Chinese Journal of Animal Nutrition, 2014 , 26(3) : 620 -629 . DOI: 10.3969/j.issn.1006-267x.2014.03.010

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