Flavin-Containing Monooxygenase 3 Genotype：Frequencies Distribution and Effects on Egg Quality in Hy-Line Brown Laying Hens

WANG Jing; WU Shugeng; ZHANG Haijun; YUE Hongyuan; QI Guanghai

doi:10.3969/j.issn.1006-267x.2014.03.011

2014 , Vol. 26 >Issue 3: 630 - 636

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.011

Poultry Nutrition

Flavin-Containing Monooxygenase 3 Genotype：Frequencies Distribution and Effects on Egg Quality in Hy-Line Brown Laying Hens

WANG Jing ,
WU Shugeng ,
ZHANG Haijun ,
YUE Hongyuan ,
QI Guanghai

Expand

Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2013-10-12

Online published: 2014-03-04

Fold

Abstract

This experiment was conducted to investigate the distribution of T329S mutation in flavin-containing monooxygenase 3 (FMO3) in Hy-Line brown laying hens, and the effects of FMO3 genotype on egg quality and egg trimethylamine (TMA) content. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by DNA sequencing assay was developed to genotype laying hens. A total of 120 genotyped laying hens were selected and fed for 6 weeks, and the egg quality and egg TMA contents were analyzed. The results showed as follows: 1) the frequencies of AA (wild type), AT (heterozygous type) and TT (mutant type) genotype were 3.4%, 76.6% and 20.0%, respectively. The allelic frequencies were not in Hardy-Weinberg equilibrium in the Hy-Line brown laying hens strain (P<0.001). 2) Egg quality was not affected by the genotype (P>0.05) with the exception of TMA contents which was higher in TT hens (P<0.05). However, yolk TMA content remained below the human detection threshold for TT hens. The results indicate that the frequency of TT hens in Hy-Line brown strain is 20.0%, which show higher egg TMA content. Egg quality is not affected by the genotype.

Key words： Hy-Line brown laying hens; FMO3; egg quality

Cite this article

WANG Jing , WU Shugeng , ZHANG Haijun , YUE Hongyuan , QI Guanghai . Flavin-Containing Monooxygenase 3 Genotype：Frequencies Distribution and Effects on Egg Quality in Hy-Line Brown Laying Hens[J]. Chinese Journal of Animal Nutrition, 2014 , 26(3) : 630 -636 . DOI: 10.3969/j.issn.1006-267x.2014.03.011

References

[1] HOBSON-FROHOCK A, LAND D G, GRIFFITHS N M, et al.Egg taints:association with trimethylamine[J].Nature, 1973, 243(5405):304-305.

[2] KRETZSCHMAR K, DAÄNICKE S, SCHMUTZ M, et al.Interactions of flavin containing monooxygenase 3 (FMO3) genotype and feeding of choline and rapeseed cake on the trimethylamine (TMA) content in egg yolks of laying hens[J].Archives of Animal Nutrition, 2009, 63(3):173-187.

[3] DÄNICKE S, UEBERSCHÄR K H, REESE K, et al.Investigations on the effects of rape oil quality, choline and methionine concentration in diets for laying hens on the trimethylamine content of the eggs, on trimethylamine metabolism and on laying performance[J].Archives of Animal Nutrition, 2006, 60(1):57-59.

[4] PEARSON A W, GREENWOOD N M, BUTLER E J, et al.The involvement of trimethylamine oxide in fish meal in the production of egg taint[J].Animal Feed Science and Technology, 1983, 8(2):119-127.

[5] WARD A K, CLASSEN H L, BUCHANAN F C.Fishy-egg Tainting is recessively inherited when brown-shelled layers are fed canola meal[J].Poultry Science, 2009, 88(4):714-721.

[6] HONKATUKIA B M, REESE K, PREISINGE R, et al.Fishy taint in chicken eggs is associated with a substitution within a conserved motif of the FMO3 gene[J].Genomics, 2005, 86(2):225-232.

[7] MARKS R, GREAVES M, PROTTEY C, et al.Trimethylaminuria:the use of choline as an aid to diagnosis[J].British Journal of Dermatology, 1977, 96(4):399-402.

[8] LUNDÉN A, GUSTAFSSON V, IMHOF M, et al.High trimethylamine concentration in milk from cows on standard diets is expressed as fishy off-flavour[J].Journal of Dairy Research, 2002, 69(3):383-390.

[9] FULTON J.Molecular genetics in a modern poultry breeding organization[J].World's Poultry Science Journal, 2008, 64(2):171-176.

[10] 王晶.FMO3基因型和胆碱对鸡蛋三甲胺含量影响的研究[D].博士学位论文.哈尔滨:东北农业大学, 2009.

[11] BOLTON W, CARTER T, JONES R M.The hen's egg:genetics of taints in eggs from hens fed on rapeseed meal[J].British Poultry Science, 1976, 17(3):313-320.

[12] PEARSON A W, BUTLER E J, FRANK CURTIS R, et al.Effect of rapeseed meal on trimethylamine metabolism in the domestic fowl in relation to egg taint[J].Journal of Science of Food and Agriculture, 1979, 30(8):799-804.

[13] LANG D H, YEUNG C K, PETER R M, et al.Isoform specificity of trimethylamine N-oxygenation by human flavin-containing monooxygenase (FMO) and P450 enzymes:selective catalysis by FMO3[J].Biochemical Pharmacology, 1998, 56(8):1005-1012.

[14] WARD A K.Genetic and dietary interactions of fishy-egg taint in brown-shelled laying hens[D].Master Thesis.Saskatoon:University of Saskatchewan, 2008.

[15] 张龙超.鸡蛋品质性状遗传参数及相关候选基因研究[D].博士学位论文.北京:中国农业大学, 2007.

[16] 王晓亮.遗传与环境因素对鸡蛋品质性状参数的影响及相关候选基因的研究[D].博士学位论文.北京:中国农业大学, 2009.

[17] GOH Y K, CLANDININ D R, ROBBLEE A R.The effect of level of sinapine in a laying ration on the incidence of fishy odor in eggs from brown-shelled egg layers[J].Canadian Journal of Animal Science, 1979, 59(2):313-316.

[18] GRIFFITHS N M, LAND D, HOBSON-FROHOCK A.Trimethylamine and egg taint[J].British Poultry Science, 1979, 20(6):555-558.

[19] WANG J, WU S G, ZHANG H J.Trimethylamine deposition in the egg yolk from laying hens with different FMO3 genotypes[J].Poultry Science, 2013, 92(3):746-752.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References