Protective Mechanisms of Dietary Pyrroloquinoline Quinine on Fatty Liver Laying Hens

ZHAO Qin; ZHANG Haijun; WU Shugeng; YUE Hongyuan; WANG Jing; QI Guanghai; SUN Linlin

doi:10.3969/j.issn.1006-267x.2014.03.014

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 3: 651 - 658

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.014

Poultry Nutrition

Protective Mechanisms of Dietary Pyrroloquinoline Quinine on Fatty Liver Laying Hens

ZHAO Qin ,
ZHANG Haijun ,
WU Shugeng ,
YUE Hongyuan ,
WANG Jing ,
QI Guanghai ,
SUN Linlin

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1. Key Laboratory of Feed Biotechnology of Ministry of Agricultural, Feed Research Institute, The Chinese Academy of Agricultural Science, Beijing 100081, China;
2. Shanghai Medical Life Science Research Center Co., Ltd., Shanghai 200032, China

Received date: 2013-10-28

Online published: 2014-03-04

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Abstract

This experiment was conducted to investigate the protective mechanisms of dietary pyrroloquinoline quinine (PQQ) on laying hens with fatty liver syndrome. Two hundred and eighty eight Hy-Line brown laying hens aged 29 weeks were randomly divided into 4 groups with 6 replicates per group and 12 hens per replicate. Hens in group Ⅰ (control group) were fed a basal diet (ME 11.03 MJ/kg; CP 16.2%), hens in group Ⅱ (pathological model control group) were fed a high-energy low-protein diet (ME 12.75 MJ/kg; CP 13.0%), and hens in groups Ⅲ and Ⅳ were fed the high-energy low-protein diet (the same as group Ⅱ) supplemented with 0.08 or 0.16 mg/kg PQQ, respectively. The experiment lasted for 4 weeks. The results showed as follows: 1) compared with the control group, high-energy low-protein diet in group Ⅱ successfully induced experimental fatty liver model; there were large quantity of fat degeneration in livers from pathological model control; the contents of triglyceride and total cholesterol in liver, and the plasma alanine aminotransferase activity in group Ⅱ were significantly increased (P<0.05). 2) Dietary PQQ strikely suppressed the elevation of contents of triglyceride and total cholesterol in liver, and the plasma alanine aminotransferase activity caused by high-energy low-protein diet (P<0.05), to the same levels as normal control. 3) Dietary PQQ significantly prevented the decrease of superoxide dismutase activity, and the increase of malondialdehyde content induced by high-energy low-protein diet (P<0.05). 4) Dietary PQQ significantly inhibited the decrease of mitochondrial relative content and the activities of citrate synthase and cytochrome C oxidase induced by high-energy low-protein diet (P<0.05). In conclusion, dietary PQQ can prevent the fatty liver syndrome induced by high-energy low-protein diets by improving mitochondrial function, and regulating the lipid metabolism and anti-oxidative activities in the liver.

Key words： pyrroloquinoline quinine; laying hen fatty liver syndrome; lipid metabolism; anti-oxidation; mitochondria

Cite this article

ZHAO Qin , ZHANG Haijun , WU Shugeng , YUE Hongyuan , WANG Jing , QI Guanghai , SUN Linlin . Protective Mechanisms of Dietary Pyrroloquinoline Quinine on Fatty Liver Laying Hens[J]. Chinese Journal of Animal Nutrition, 2014 , 26(3) : 651 -658 . DOI: 10.3969/j.issn.1006-267x.2014.03.014

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