Effects of Heat Stress on Expressions of Tight Junction Proteins in Ruminal Mucosa of Dairy Goats

MA Yanfen; DU Ruiping; GAO Min

doi:10.3969/j.issn.1006-267x.2014.03.029

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 3: 768 - 775

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.029

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Effects of Heat Stress on Expressions of Tight Junction Proteins in Ruminal Mucosa of Dairy Goats

MA Yanfen ,
DU Ruiping ,
GAO Min

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Animal Nutrition and Feed Research Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry, Hohhot 010031, China

Received date: 2013-09-07

Online published: 2014-03-04

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Abstract

This study was conducted to study the effects of heat stress (HS) on expressions of tight junction proteins in ruminal mucosa of dairy goats. Eight healthy dietary goats with similar body weight, age, parity and milk yield at mid to late-locating period were randomly divided into control group and heat stress (HS) group with 4 goats in each group. Environmental temperature and humidity during feeding period were artificially controlled. The experiment lasted for 45 days. Knapsack and abdominal sac samples were collected at 30 and 45 days of experiment to determine protein structural distribution, gene mRNA expression levels and protein expression levels of claudin and zonula occluden 1 (ZO-1) using the methods of immunohistochemisty, real-time quantitative PCR and West-bloting, respectively. The results showed as follows: compared with the control group, expression levels and signal intensity of claudin and ZO-1 proteins in knapsack and abdominal in HS group (30 days) tened to be decreased, and those in HS group (45 days) were further decresed. Compared with the control group, mRNA expression levels of claudin and ZO-1 genes in knapsack and abdominal in HS group (30 days) were significantly decreased (P<0.05), and those in HS group (45 days) were significantly lower than those in HS group (30 days) (P<0.05). In conclusion, the damage of heat stress on structure and integrity of ruminal mucosa is due to the declined expression of tight junction proteins.

Key words： heat stress; dairy goats; ruminal mucosa; tight junction protein

Cite this article

MA Yanfen , DU Ruiping , GAO Min . Effects of Heat Stress on Expressions of Tight Junction Proteins in Ruminal Mucosa of Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2014 , 26(3) : 768 -775 . DOI: 10.3969/j.issn.1006-267x.2014.03.029

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