Effects of Bacillus amyloliquefaciens SC06 on Intestinal Bacterial Enzyme Activities and Intestinal Mucosal Barrier Function of Immunosuppressive Mice

DU Wei; HUANG Qin; FU Aikun; YU Dongyou; LI Weifen

doi:10.3969/j.issn.1006-267x.2014.03.035

2014 , Vol. 26 >Issue 3: 819 - 826

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.035

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Effects of Bacillus amyloliquefaciens SC06 on Intestinal Bacterial Enzyme Activities and Intestinal Mucosal Barrier Function of Immunosuppressive Mice

DU Wei ,
HUANG Qin ,
FU Aikun ,
YU Dongyou ,
LI Weifen

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Key Laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Received date: 2013-09-17

Online published: 2014-03-04

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Abstract

This study was conducted to study the effects of Bacillus amyloliquefaciens SC06 on intestinal bacterial enzyme activities and intestinal mucosal barrier function of immunosuppressive mice. The model of immunosuppressive mice was built by intraperitoneal injection of cyclophosphamide (CTX) for 3 days continuously. A total of 120 BALB/c mice (4-week-old, female and body weight of 10 to 16 g) were randomly divided into 3 groups with 4 replicates per group and 10 mice per replicate. The mice in control group and immunosuppressive group were all fed basal diet, and those in Bacillus group were fed the basal diet supplemented with Bacillus amyloliquefaciens SC06 (living bacteria count was 1×10⁵ CFU/g). On the 1st to 3rd day of the experiment, the mice in immunosuppressive group and Bacillus group were intraperitoneally injected with 80 μg/g BW of CTX for 3 consecutive days. The experiment lasted for 60 days. The results showed as follows: compared with the control group, the activities of bacterial enzymes (including α-glucosidase, β-glucosidase, β-glucuronidase, α-galactosidase and β-galactosidase) in cecal and small intestinal contents and diamine oxidase (DAO) in small intestinal lavage fluid were significantly decreased (P<0.01), the content of nitric oxide (NO) and the activity of inducible nitric oxide synthase (iNOS) in small intestinal lavage fluid were significantly increased (P<0.01), and the contents of pro-inflammatory cytokines—aproliferation inducingligand (APRIL), interferon-γ (IFN-γ), interleukin-6 (IL-6) and interleukin-12 (IL-12) and anti-inflammatory cytokine—interleukin-10 (IL-10) in small intestinal lavage fluid were significantly increased (P<0.01). It indicated that the intestinal flora of mice was disordered, and the intestinal mucosa epithelial cells and the intestinal mucosal barrier function were damaged by intraperitoneal injection of CTX. Bacillus amyloliquefaciens SC06 could significantly increase the activities of β-glucuronidase and α-glucosidase in cecal contents and β-glucuronidase in small intestinal contents (P<0.01), and significantly increase the activities of myeloperoxidase (MPO) and secretory phospholipase A2 (sPLA2) in small intestinal lavage fluid (P<0.01 and P<0.05, respectively). The results indicate that Bacillus amyloliquefaciens SC06 can increase the intestinal probiotic count in a certain extent, but only can partially recover the intestinal mucosal barrier function.

Key words： Bacillus amyloliquefaciens SC06; immunosuppression; mice; bacterial enzyme activities; intestinal mucosal barrier function

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DU Wei , HUANG Qin , FU Aikun , YU Dongyou , LI Weifen . Effects of Bacillus amyloliquefaciens SC06 on Intestinal Bacterial Enzyme Activities and Intestinal Mucosal Barrier Function of Immunosuppressive Mice[J]. Chinese Journal of Animal Nutrition, 2014 , 26(3) : 819 -826 . DOI: 10.3969/j.issn.1006-267x.2014.03.035

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