Effects of Qyrroloquinoline Quinine on Performance, Egg Quality, Plasma Lipid Metabolism and Antioxidant Ability of Laying Hens Fed High-Energy Low-Protein Diets

ZHAO Qin; ZHANG Haijun; WU Shugeng; YUE Hongyuan; WANG Jing; QI Guanghai; SUN Linlin

doi:10.3969/j.issn.1006-267x.2014.04.008

2014 , Vol. 26 >Issue 4: 885 - 892

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.04.008

Poultry Nutrition

Effects of Qyrroloquinoline Quinine on Performance, Egg Quality, Plasma Lipid Metabolism and Antioxidant Ability of Laying Hens Fed High-Energy Low-Protein Diets

ZHAO Qin ,
ZHANG Haijun ,
WU Shugeng ,
YUE Hongyuan ,
WANG Jing ,
QI Guanghai ,
SUN Linlin

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1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;
2. Shanghai Medical Life Science Research Center Co., Ltd., Shanghai 200032, China

Received date: 2013-10-28

Online published: 2014-04-01

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Abstract

This experiment was conducted to investigate the effects of pyrroloquinoline quinine (PQQ) on performance, egg quality, plasma lipid metabolism and antioxidant ability of laying hens fed high-energy low-protein diets. Two hundred and eighty-eight Hy-Line brown laying hens aged 29 weeks were randomly divided into 4 groups with 6 replicates per group and 12 hens per replicate. Hens in group Ⅰ (control group) were fed a basal diet (metabolic energy 11.03 MJ/kg; crude protein 16.2%), hens in group Ⅱ (pathological model control group) were fed a high-energy low-protein diet (metabolic energy 12.75 MJ/kg; crude protein 13.0%), and hens in groups Ⅲ and Ⅳ were fed the high-energy low-protein diet (the same as group Ⅱ) supplemented with 0.08 and 0.16 mg/kg PQQ, respectively. The experiment lasted for 4 weeks. The results showed as follows: 1) compared with the control group, the high-energy low-protein diet induced fatty liver, the liver fat rate in group Ⅱ was significantly increased (P<0.05), and the contents of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in plasma in group Ⅱ were significantly increased (P<0.05). 2) Dietary PQQ could significantly decrease the increase of average daily feed intake and the ratio of feed to egg of laying hens fed high-energy low-protein diets (P<0.05), it could significantly inhibit the decrease of albumen height, Haugh unit and yolk color and the increase of the contents of TG, TC and LDL-C and lactate dehydrogenase (LDH) activity in plasma (P<0.05), and all the above indexes could get the same levels as control group. 3) Dietary PQQ could significantly inhibit the decrease of plasma superoxide dismutase activity and the increase of plasma malondialdehyde content of laying hens fed high-energy low-protein diets (P<0.05). In conclusion, dietary PQQ can decrease the liver fat content, improve the lipid metabolism and antioxidant ability of laying hens.

Key words： pyrroloquinoline quinine; laying hen fatty liver syndrome; high-energy low-protein diet; lipid metabolism; antioxidant

Cite this article

ZHAO Qin , ZHANG Haijun , WU Shugeng , YUE Hongyuan , WANG Jing , QI Guanghai , SUN Linlin . Effects of Qyrroloquinoline Quinine on Performance, Egg Quality, Plasma Lipid Metabolism and Antioxidant Ability of Laying Hens Fed High-Energy Low-Protein Diets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(4) : 885 -892 . DOI: 10.3969/j.issn.1006-267x.2014.04.008

References

[1] BUTLER E J.Fatty liver diseases in the domestic fowl-a review[J].Avian Pathology,1976,5(1):1-14.

[2] 郭小权,曹华斌,胡国良,等.高能量低蛋白质日粮中添加生物素对蛋鸡脂类代谢的影响[J].中国兽医学报,2012,32(5):754-758.

[3] 郭小权,胡国良,曹华斌,等.高能低蛋白日粮致脂肪肝出血综合征鸡抗氧化能力和肝损伤的研究[J].中国兽医学报,2010,30(6):829-832.

[4] BAUERLY K,HARRIS C,CHOWANADISAI W,et al.Altering pyrroloquinoline quinone nutritional status modulates mitochondrial,lipid,and energy metabolism in rats[J].PLoS One,2011,6(7):e21779.

[5] 闵向荣,赵永芳.PQQ对四氯化碳和乙醇引起大鼠肝损伤的保护作用[J].武汉大学学报,2001(6):757-760.

[6] 徐磊,张海军,武书庚,等.吡咯喹啉醌对蛋鸡生产性能、蛋品质及抗氧化功能的影响[J].动物营养学报,2011,23(8):1370-1377.

[7] FOLCH J,LEES M,SLOANE G H.A sample method for the isolation ang purification of total lipids from animal tissues[J].The Journal of Biological Chemistry,1956,226:497-509.

[8] YOUSEFI M,SHIVAZAD M,SOHRABI-HAGHDOOST I.Effect of dietary factors on induction of fatty liver-hemorrhagic syndrome and its diagnosis methods with use of serum and liver parameters in laying hens[J].International Journal of Poultry Science,2005,4(8):568-572.

[9] 曹华斌,胡国良,郭小权,等.高能低蛋白日粮饲养蛋鸡肝脏脂肪沉积及其组织病理学观察[J].中国兽医学报,2010,30(7):1009-1012.

[10] 花象柏,刘鑫,邵莹,等.产蛋鸡实验性脂肪肝综合症及肝脏病理学观察[J].江西畜牧兽医杂志,1993(1):3-5.

[11] BAUERLY K A,STORMS D H,HARRIS C B,et al.Pyrroloquinoline quinone nutritional status alters lysine metabolism and modulates mitochondrial DNA content in the mouse and rat[J].Biochimica et biophysica Acta General Subjects,2006,1760(11):1741-1748.

[12] STEINBERG F,STITES T E,ANDERSON P,et al.Pyrroloquinoline quinone improves growth and reproductive performance in mice[J].Experimental Biology and Medicine,2003,228(2):160-166.

[13] STEINBERG F M,GERSHWIN M E,BUCKER R B,et al.Dietary pyrroloquinoline quinone:growth and immune response in BALB/c mice[J].The Journal of Nutrition,1994,124(5):744-753.

[14] DIAZ G,SQUIRES E,JULIAN R.The use of selected plasma enzyme activities for the diagnosis of fatty liver-hemorrhagic syndrome in laying hens[J].Avian Diseases,1999,43(4):768-773.

[15] 成嘉,符贵红,刘芳,等.重金属铅対鲫鱼乳酸脱氢酶和过氧化氢酶活性的影响[J].生命科学,2006,10(4):372-376.

[16] CHOWANADISAI W,BAUERLY K A,TCHAPARIAN E,et al.Pyrroloquinoline quinone stimulates mitochondrial biogenesis through cAMP response element-binding protein phosphorylation and increased PGC-1α expression[J].The Journal of Biological Chemistry,2010,285(1):142-152.

[17] HE K,NUKADA H,URAKAMI T,et al.Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ):implications for its function in biological systems[J].Biochemical Pharmacology,2003,65(1):67-74.

[18] 徐磊.日粮中添加吡咯喹啉醌对产蛋鸡生产性能和抗氧化机能的影响[D].硕士学位论文.北京:中国农业科学院,2011:27-29.

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