This experiment was conducted to study the effects of Astragalus polysaccharides (APS) on serum immunocyte factor contents and small intestine inducible nitric oxide synthase (iNOS) mRNA expression of broilers. A total of 300 one-day-old healthy Avian broilers were randomly divided into 5 groups with 6 replicates per group and 10 broilers per replicate. Broilers in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diet supplemented with 0.1%, 0.5%, 1.0% and 2.0% APS, respectively. The experiment lasted for 42 days. The results showed as follows: 1) dietary supplementation of APS increased the average weight and the average daily gain of broilers, and decreased the ratio of feed to gain, and the 1.0% APS group had the best effect. 2) Dietary supplementation of APS increased the contents of interleukin-1, interleukin-2 and tumor necrosis factor-α in serum of broilers, the contents of interleukin-1 and tumor necrosis factor-α in serum showed a quadratic increasing at 42 days of age (P<0.05), and the contents of tumor necrosis factor-α and interleukin-1 in serum in 0.5% and 1.0% APS groups had the best effect at 42 days of age, respectively. 3) Dietary supplementation of APS increased the nitric oxide content and iNOS activity in serum, and increased the small intestine iNOS mRNA expression, the small intestine iNOS mRNA expression showed a quadratic increasing at 42 days of age (P<0.05), and the 1.0% APS group had the best effect. In conclusion, the regulation of APS on immune function of broilers may relate to the expression of iNOS mRNA, iNOS activity and the secretion of immunocyte factors.
ZHAO Tianzhang
,
LI Huiying
,
WANG Zhigang
,
LI Jun
. Effects of Astragalus Polysaccharides on Serum Immunocyte Factor Contents and Small Intestine Inducible Nitric Oxide Synthase mRNA Expression of Broilers[J]. Chinese Journal of Animal Nutrition, 2014
, 26(4)
: 1011
-1018
.
DOI: 10.3969/j.issn.1006-267x.2014.04.021
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