Optimization of Compound Bacteria Solid-State Fermentation Medium and Fermentation Process

LIU Guojuan; LIU Dacheng; LU Chunfang; HU Honglian; GAO Min

doi:10.3969/j.issn.1006-267x.2014.04.022

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 4: 1019 - 1027

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.04.022

Feed Science and Technology

Optimization of Compound Bacteria Solid-State Fermentation Medium and Fermentation Process

LIU Guojuan ,
LIU Dacheng ,
LU Chunfang ,
HU Honglian ,
GAO Min

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1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, China;
3. Animal Nutrition Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2013-11-07

Online published: 2014-04-01

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Abstract

This experiment was conducted to study the optimization of compound bacteria solid-state fermentation medium and fermentation process. The mixing experimental design and uniform experimental design by DPS software were used, combined into 14 kinds of solid-state fermentation medium and 14 kinds of fermentation process. Determining the contents of viable count, β-glucan, mannan and organic acid to filter the fine components and optimized the fermentation process. The results showed as follows: 1) the optimized compound bacteria solid-state fermentation medium was wheat bran 52.69%, corn bran 20.45%, corn flour 16.07%, rice bran 4.09%, cottonseed meal 1.86%, soybean meal 3.99% and inorganic salt 0.85%; 2) the optimized fermentation process was moisture 60%, pH 8.0, temperature 30 ℃ and time 46 h. The indexes of optimal combination were viable count 6.71×10⁸ CFU/g, glucan content 279.83 mg/g, mannan content 156.82 mg/g, tartaric acid content 2.78 mg/g, malic acid content 8.28 mg/g, citric acid content 1.34 mg/g and butanedioic acid content 2.54 mg/g. In conclusion, this experiment optimizes the compound bacteria solid-state fermentation medium and fermentation process.

Key words： compound bacteria; active material; fermentation medium; fermentation process

Cite this article

LIU Guojuan , LIU Dacheng , LU Chunfang , HU Honglian , GAO Min . Optimization of Compound Bacteria Solid-State Fermentation Medium and Fermentation Process[J]. Chinese Journal of Animal Nutrition, 2014 , 26(4) : 1019 -1027 . DOI: 10.3969/j.issn.1006-267x.2014.04.022

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