Effects of Arginine and N-Carbamylglutamate on Lipid Metabolism of Huanjiang Mini-Pigs

ZHOU Xiaoli; LIU Junfeng; WU Chen; GENG Meimei; KONG Xiangfeng

doi:10.3969/j.issn.1006-267x.2014.04.026

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 4: 1055 - 1060

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.04.026

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Effects of Arginine and N-Carbamylglutamate on Lipid Metabolism of Huanjiang Mini-Pigs

ZHOU Xiaoli ,
LIU Junfeng ,
WU Chen ,
GENG Meimei ,
KONG Xiangfeng

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1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Guiyang University, Guiyang 550005, China;
3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2013-10-10

Online published: 2014-04-01

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Abstract

This study was conducted to determine the effects of dietary supplemented with L-arginine (Arg) or N-carbamylglutamate (NCG) on intramuscular contents of fatty acids and the gene expression levels related to lipid metabolism in Huanjiang mini-pigs. Tweety-four Huanjiang mini-pigs with initial body weight (BW) of (28.14±3.45) kg were randomly assigned into 3 groups with 4 replicates in each group and 2 pigs per replicate, representing Arg, NCG and control groups, and pigs were supplemented with 0.83% L-Arg + 0.88% rice bran, 0.08% NCG+1.71% L-alanine, and 1.71% L-alanine in a basal diet, respectively. After a 45-day feeding period, the liver and longissimus dorsi were obtained for determining intramuscular contents of fatty acids and the gene expression levels related to lipid metabolism. The results showed that compared with the control group, dietary supplemented with Arg significantly decreased muscle content of C18:0 (P<0.05), as well as the expression levels of acetyl-CoA carboxylase α (ACCα) mRNA in liver, and ACCα and peroxisome proliferator activated receptor-γ (PPARγ) mRNA in muscle (P<0.05), while significantly increased the expression level of PPARγ mRNA in liver (P<0.05); dietary supplementation of NCG significantly decreased the expression levels of ACCα and lipoprteinlipase (LPL) mRNA in liver (P<0.05), as well as ACCα, fatty acid synthetase (FAS) and LPL mRNA in muscle, while significantly increased the expression level of PPARγ mRNA in muscle (P<0.05). These results indicate that dietary supplemented with Arg or NCG have a regulating effect on gene expression related to lipid metabolism in the liver and longissimus dorsi, and decrease the content of C18:0 in longissimus dorsi.

Key words： arginine; N-carbamylglutamate; Huanjiang mini-pig; fatty acid; lipid metabolism

Cite this article

ZHOU Xiaoli , LIU Junfeng , WU Chen , GENG Meimei , KONG Xiangfeng . Effects of Arginine and N-Carbamylglutamate on Lipid Metabolism of Huanjiang Mini-Pigs[J]. Chinese Journal of Animal Nutrition, 2014 , 26(4) : 1055 -1060 . DOI: 10.3969/j.issn.1006-267x.2014.04.026

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