Aquaculture Nutrition

The Variation of Digestive Enzyme Activities of Carassius auratus during Early Development and the Effects of Exogenous Vitamin C

  • JIANG Zuoyu ,
  • YAO Junjie ,
  • XIONG Hualong
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  • Collage of Animal Sciences, Guizhou University, Guiyang 550025, China

Received date: 2013-11-25

  Online published: 2014-05-05

Abstract

This experiment was conducted to study the variation of digestive enzyme activities of Carassius auratus during early development and the effects of exogenous vitamin C. Mature eggs from Carassius auratus were selected and cross-fertilized using Cyprinus carpio by the method of artificial dried fertilization. The eggs were divided into two groups, and the vitamin C concentration in water for incubation was 0 and 30 mg/L, respectively. Samples were collected at mature eggs, fertilized eggs, mid-gastrula, crystal appear, mixed nutrition and exogenous nutrition stages to determine the variation of digestive enzyme activities. The results showed as follows: 1) vitamin C was contributed to the deposition of protein in Carassius auratus during early development. 2) After cross-fertilization, the activity of amylase was significantly higher than that of mature eggs (P<0.05). 3) From mid-gastrula stage to exogenous nutrition stage, the activities of trypsin and amylase were increased with development, the differences among stages were significant (P<0.05); the activities of pepsin, lipase and cellulase had no obvious rise from mid-gastrula stage to crystal appear stage (P>0.05), but were raised from crystal appear stage to exogenous nutrient stage, the differences were significant (P<0.05). 4) The inhabitation in 30 mg/L vitamin C solution significantly increased the activities of trypsin, amylase, lipase and pepsin, but had no obvious effect on cellulase activity. The results indicate that amylase priority decomposition carbohydrates in yolk of mature eggs from Cyprinus carpio to provide energy for embryonic development rapidly after cross-fertilization; trypsin, amylase, pepsin and lipase play major roles in decomposition yolk nutrients, organ formation and the development of larvae; 30 mg/L vitamin C solution can significantly increase the activities of trypsin, amylase, pepsin and lipase, and elevate the deposition of protein.

Cite this article

JIANG Zuoyu , YAO Junjie , XIONG Hualong . The Variation of Digestive Enzyme Activities of Carassius auratus during Early Development and the Effects of Exogenous Vitamin C[J]. Chinese Journal of Animal Nutrition, 2014 , 26(5) : 1246 -1253 . DOI: 10.3969/j.issn.1006-267x.2014.05.015

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