Effects of Dietary n-3 Highly Unsaturated Fatty Acid Content on Growth Performance and Fatty Acid Composition of Juvenile Swimming Crab (Portunus trituberculatus)

ZHANG Wen; XIE Fengjun; JIN Min; ZHOU Qicun; LI Ming; HAN Changmao

doi:10.3969/j.issn.1006-267x.2014.05.016

2014 , Vol. 26 >Issue 5: 1254 - 1264

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.05.016

Aquaculture Nutrition

Effects of Dietary n-3 Highly Unsaturated Fatty Acid Content on Growth Performance and Fatty Acid Composition of Juvenile Swimming Crab (Portunus trituberculatus)

ZHANG Wen ,
XIE Fengjun ,
JIN Min ,
ZHOU Qicun ,
LI Ming ,
HAN Changmao

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1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Ningbo Xinyi Live Aquatic Products Co., Ltd., Ningbo 315211, China

Received date: 2013-11-21

Online published: 2014-05-05

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Abstract

This experiment was conducted to study the effects of dietary n-3 highly unsaturated fatty acid (HUFA) content on growth performance and fatty acid composition of juvenile swimming crab (Portunus tribueculatus). Six isonitrogenous and isoenergetic experimental diets were formulated in which n-3 HUFA content was 0.74%, 1.14%, 1.55%, 1.94%, 2.35% and 2.76% (dry matter basis), respectively, and C22∶6n-3 (DHA)/C20∶5n-3 (EPA) was 1.1. Juvenile crabs with an initial body weight about 2.20 g were selected as experimental animals, and all of them were cultured in rectangle plastic baskets in outdoor cement pit for 8 weeks. Each of experimental diets was fed 60 juvenile crabs, and 20 of them as a replicate. The results showed as follows: weight gain rate, specific growth rate, feed efficiency and molt rate in 2.35% group were all the highest. Among of them, the weight gain rate and specific growth rate in 2.35% group were significantly higher than those in 0.74%, 1.14% and 1.55% groups (P<0.05), the feed efficiency in 2.35% group was significantly higher than that in other groups (P<0.05), and the molt rate in 2.35% group was significantly higher than that in 0.74%, 1.14% and 1.94% groups (P<0.05). Dietary n-3 HUFA content had no significant influence on hepatopancreas index (P>0.05). There were no significant differences in the contents of moisture, crude protein and ash of juvenile crabs among all groups (P>0.05), but the content of crude lipid in 0.74% group was significantly lower than that in other groups (P<0.05). Dietary n-3 HUFA content affected fatty acid composition in muscle and hepatopancreas, and significant positive correlation relationships were found between dietary n-3 HUFA content and n-3 HUFA content in muscle and hepatopancreas (P<0.01). Using specific growth rate as an evaluating index, dietary optimal n-3 HUFA content of juvenile swimming crab is estimated to be 2.357% (dry matter basis) by broken-line regression analysis.

Key words： n-3 highly unsaturated fatty acid; swimming crab (Portunus tribueculatus); growth performance; fatty acid composition

Cite this article

ZHANG Wen , XIE Fengjun , JIN Min , ZHOU Qicun , LI Ming , HAN Changmao . Effects of Dietary n-3 Highly Unsaturated Fatty Acid Content on Growth Performance and Fatty Acid Composition of Juvenile Swimming Crab (Portunus trituberculatus)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(5) : 1254 -1264 . DOI: 10.3969/j.issn.1006-267x.2014.05.016

References

[1] 高红建,何中央,丁雪燕,等.三疣梭子蟹幼蟹配合饲料中蛋白质、脂肪、粗纤维适宜含量研究[J].渔业现代化,2009,36(1):26-29.

[2] CATACUTAN M R.Growth and body composition of juvenile mud crab,Scylla serrata,fed different dietary protein and lipid levels and protein to energy ratios[J].Aquaculture,2002,208(1/2):113-123.

[3] SHEEN S S,WU S W.The effects of dietary lipid levels on the growth response of juvenile mud crab Scylla serrata[J].Aquaculture,1999,175(1/2):143-153.

[4] 常国亮,成永旭,于智勇,等.脂类营养对中华绒螯蟹幼蟹肝胰腺超微结构的影响[J].河南师范大学学报:自然科学版,2011,39(4):108-111.

[5] ROMANO N,ZENG C S.The effects of salinity on the survival,growth and haemolymph osmolality of early juvenile blue swimmer crabs,Portunus pelagicus[J].Aquaculture,2006,260(1/2/3/4):151-162.

[6] HOLME M H,SOUTHGATE P C,ZENG C S.Survival,development and growth response of mud crab,Scylla serrata,megalopae fed semi-purified diets containing various fish oil∶corn oil ratios[J].Aquaculture,2007,269(1/2/3/4):427-435.

[7] TANDLER A,WATANABE T,SATOH S,et al.The effect of food deprivation on the fatty acid and lipid profile of red seabream (Pagrus major) larvae[J].British Journal of Nutrition,1989,62(2):349-361.

[8] LIU J K,XU S H.Nutrient requirement of marine fish larvae for essential fatty acids and phospholipids[J].Marine Sciences,2006,30(11):75-81.

[9] SARGENT J R,HENDERSON R J,TOCHER D R.The lipids[M]//HALVER J E.Fish nutrition.2nd ed.London:Academic Press,1989:153-218.

[10] WU F C,TING Y Y,CHEN H Y.Dietary docosahexaenoic acid is more optimal than eicosapentaenoic acid affecting the level of cellular defence responses of the juvenile grouper Epinephelus malabaricus[J].Fish & Shellfish Immunology,2003,14(3):223-238.

[11] XU H G,AI Q H,MAI K S,et al.Effects of dietary arachidonic acid on growth performance,survival,immune response and tissue fatty acid composition of juvenile Japanese seabass,Lateolabrax japonicas[J].Aquaculture,2010,307(1/2):75-82.

[12] KITAJIMA C,ARAKAWA T,OOWA G,et al.Dietary value for red seabream of rotifer,Brachionus plicatilis,cultured with a new type of yeast[J].Bulletin of the Japanese Society of Scientific Fisheries,1980,46(1):43-46.

[13] KITAJIMA C,YOSHIDA M,WATANABE T.Dietary value for ayu,Plecoglossus altivelis,of rotifer,Brachionus plicatilis,cultured with baker's yeast,Saccaromyces cerelisiae,supplemented with cuttlefish liver oil[J].Bulletin of the Japanese Society of Scientific Fisheries,1980,46(1):47-50.

[14] WATANABE T,TAMIYA T,OKA A,et al.Improvement of dietary value of live foods for fish larvae by feeding them on ω3 highly unsaturated fatty acids and fat-soluble vitamins[J].Bulletin of the Japanese Society of Scientific Fisheries,1983,49(3):471-479.

[15] KANAZAWA A.Essential fatty acid and lipid requirements of fish[M]//COWEY C B,MACKIE A M,BELL J G.Nutrition and feeding in fish.London:Academic Press,1985:281-298.

[16] IZQUIERDO M S,WATANABE T,TAKEUCHI T,et al.Requirement of larval red seabream Pagrus major for essential fatty acids[J].Nippon Suisan Gakkaishi,1989,55(5):859-867.

[17] KOVEN W M,KISSIL G W,TANDLER A.Lipid and n-3 requirement of Sparus aurata larvae during starvation and feeding[J].Aquaculture,1989,79(1/2/3/4):185-191.

[18] KOVEN W M,TANDLER A,KISSIL G W,et al.The effect of dietary (n-3) highly unsaturated fatty acids on growth,survival and swim bladder development in Sparus aurata larvae[J].Aquaculture,1990,91(1/2):131-141.

[19] KOVEN W M,TANDLER A,KISSIL G W,et al.The importance of n-3 highly unsaturated fatty acids for growth in larval Sparus aurata and their effect on survival,lipid composition and size distribution[J].Aquaculture,1992,104(1/2):91-104.

[20] IZQUIERDO M S.Essential fatty acid requirements of cultured marine fish larvae[J].Aquaculture Nutrition,1996,2(4):183-191.

[21] NARCISO L,POUSO-FERREIRA P,PASSOS A,et al.HUFA content and DHA/EPA improvements of Artemia sp.with commercial oils during different enrichment periods[J].Aquaculture Research,1999,30(1):21-24.

[22] PALACIOS E,BONILLA A,PÉREZ A,et al.Influence of highly unsaturated fatty acids on the responses of white shrimp (Litopenaeus vannamei) postlarvae to low salinity[J].Journal of Experimental Marine Biology and Ecology,2004,299(2):201-215.

[23] TAKEUCHI T,NAKAMOTO Y,HAMASAKI K,et al.Requirement of n-3 highly unsaturated fatty acids for larval swimming crab Portunus trituberculatus[J].Nippon Suisan Gakkaishi,1999,65(5):797-803.

[24] BLIGH E G,DYER W J.A rapid method of total lipid extraction and purification[J].Canadian Journal of Anesthesia,1959,37(8):911-917.

[25] 汪正范.色谱定性与定量[M].北京:化学工业出版社,2000:168-169.

[26] 艾春香,林琼武,李少菁,等.蟹类的营养需求研究及其配合饲料研制[J].厦门大学学报:自然科学版,2006,45(增刊2):205-212.

[27] SUPRAYUDI M A,TAKEUCHI T,HAMASAKI K.Essential fatty acids for larval mud crab Scylla serrata:implications of lack of the ability to bioconvert C18 unsaturated fatty acids to highly unsaturated fatty acids[J].Aquaculture,2004,231(1/2/3/4):403-416.

[28] 徐新章,何珍秀,付培峰.不同脂肪源对幼蟹生长的影响[J].饲料工业,1997,18(5):16-18.

[29] 温小波,陈立侨.磷脂和胆固醇在虾蟹类营养中的研究进展[J].淡水渔业,2000,30(5):25-27.

[30] 翁幼竹,李少菁,王桂忠.从锯缘青蟹幼体及其饵料的含脂情况探讨其脂营养需求[J].海洋学,2003,25(增刊2):88-94.

[31] SUBRAMONIAM T.Crustacean ecdysteriods in reproduction and embryogenesis[J].Comparative Biochemistry and Physiology,2000,125:135-156.

[32] KOBAYASHI T,TAKEUCHI T,ARAI D,et al.Suitable dietary levels of EPA and DHA for larval mud crab during Artemia feeding period[J].Nippon Suisan Gakkaishi,2000,66(6):1006-1013.

[33] HOULIHAN D F,MCCARTHY I D,CARTER C G,et al.Protein turnover and amino acid flux in fish larvae[J].ICES Marine Science Symposia,1995,201:87-99.

[34] 薛敏,李爱杰,张显娟.牙鲆幼鱼对EPA和DHA的营养需求[J].水产学报,2004,28(3):285-291.

[35] COPEMAN L A,PARRISH C C,BROWN J A,et al.Effects of docosahexaenoic,eicosapentaenoic and arachidonic acids on the early growth,survival,lipid composition and pigmentation of yellowtail flounder (Limanda ferruginea):a live food enrichment experiment[J].Aquaculture,2002,210(1/2/3/4):285-304.

[36] FOUNTOULAKI E,ALEXIS M N,NENGAS I,et al.Effects of dietary arachidonic acid (20∶4n-6) on growth,body composition and tissue fatty acid profile of gilthead bream fingerlings (Sparus aurata L.)[J].Aquaculture,2003,225(1/2/3/4):309-323.

[37] SUI L,WILLE M,CHENG Y,et al.The effect of dietary n-3 HUFA levels and DHA∶EPA ratios on the growth,survival and osmotic stress tolerance of Chinese mitten crab Eriocheir sinensis larvae[J].Aquaculture,2007,273(1):139-150.

[38] REZEK T C,WATANABE W O,HAREL M,et al.Effects of dietary docosahexaenoic acid (22∶6n-3) and arachidonic acid (20∶4n-6) on the growth,survival,stress resistance and fatty acid composition in black sea bass Centropristis striata (Linnaeus 1758) larvae[J].Aquaculture Research,2010,41(9):1302-1314.

[39] PETERSEN S F,FALK-PETERSEN I B,SARGENT G R,et al.Lipid class and fatty acid composition of eggs from the Atlantic halibut (Hippoglossus hippoglossus)[J].Aquaculture,1986,52(3):207-211.

[40] MOURENTE G,RODRIGUEZ A,TOCHER D R,et al.Effects of dietary docosahexaenoic acid (DHA;22∶6n-3) on lipid and fatty acid compositions and growth in gilthead sea bream (Sparus aurata L.) larvae during first feeding[J].Aquaculture,1993,112(1):79-98.

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