Short Communications

Effects of Protease on Growth Performance, Amino Acid Apparent Digestibility and Intestinal Morphology of Broilers Aged from 8 to 21 Days

  • ZHOU Liang ,
  • ZHANG Haijun ,
  • WANG Jing ,
  • ZHENG Xuyang ,
  • QI Guanghai ,
  • WU Shugeng
Expand
  • 1. Key Laboratory of Biotechnology of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. DSM (China) Co., Ltd., Beijing 100027, China

Received date: 2013-11-15

  Online published: 2014-05-05

Abstract

This experiment was conducted to study the effects of protease on growth performance, amino acid apparent digestibility and intestinal morphology of broilers aged from 8 to 21 days. A total of 560 seven-day-old healthy Arbor Acres broilers with a similar body weight were randomly allocated to 5 groups with 8 replicates per group and 14 chickens per replicate. Broilers in the control group were fed a basal diet, and broilers in the experimental groups were fed the basal diet supplemented with 0.01%, 0.02%, 0.04% and 0.08% protease, respectively. The experiment lasted for 14 days. The results showed as follows: 1) the average daily feed intake of broilers in protease groups was significantly higher than that in control group (P<0.05), and the average daily gain of broilers in 0.02% protease group was significantly higher than that in control group (P<0.05). 2) The apparent digestibility of crude protein and amino acids (except leucine and isoleucine) showed a quadratic curve of rising (P<0.05), the crude protein apparent digestibility of broilers in 0.02% protease group was significantly higher than that in control group (P<0.05), and the apparent digestibility of asparagic acid, threonine, serine and alanine in 0.01% and 0.02% protease groups was significantly higher than that in control group (P<0.05). 3) With the increase of protease supplemental level, the crypt depth of duodenum showed a linear curve of rising (P<0.05), and the ratio of villus height to crypt depth (V/C) showed a linear curve of falling (P<0.05); the villus height of jejunum showed a quadratic curve of rising (P<0.05), and the crypt depth showed a linear curve of rising (P<0.05). It is concluded that adding 0.01% to 0.02% protease to the corn-soybean meal-meat and bone meal diet can promote the development of small intestine of broilers in the early stage, improve the apparent digestibility of crude protein and most of amino acids, and then improve the growth performance.

Cite this article

ZHOU Liang , ZHANG Haijun , WANG Jing , ZHENG Xuyang , QI Guanghai , WU Shugeng . Effects of Protease on Growth Performance, Amino Acid Apparent Digestibility and Intestinal Morphology of Broilers Aged from 8 to 21 Days[J]. Chinese Journal of Animal Nutrition, 2014 , 26(5) : 1362 -1369 . DOI: 10.3969/j.issn.1006-267x.2014.05.030

References

[1] GLITSØ V,PONTOPPIDAN K,KNAP I,et al.Development of a feed protease[J].Industrial Biotechnology,2012,8(4):172-175.  

[2] ANGEL C R,SAYLOR W,VIEIRA S L,et al.Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens[J].Poultry Science,2011,90(10):2281-2286.  

[3] 游金明,瞿明仁,黎观红,等.复合蛋白酶和甘露寡糖对肉鸡生长性能的影响及其互作效应研究[J].动物营养学报,2008,20(5):567-571.

[4] PÉRON A,OWUSU-ASIEDU A,DEBICKI-GARNIER A M,et al.Effect of a xylanase,amylase and protease combination on the performance of broiler chickens fed corn-based diets[C]//Actes des 9èmes journées de la recherche avicole.Paris:Institut Technique de l'Aviculture,2011.

[5] FREITAS D M,VIEIRA S L,ANGEL C R,et al.Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease[J].The Journal of Applied Poultry Research,2011,20(3):222-334.

[6] FRU-NJI F,LUENTER A,FISCHER M,et al.A feed serine protease improves broiler performance and increases protein and energy digestibility[J].The Journal of Poultry Science,2011,48(4):239-246.  

[7] 许梓荣,钱利纯.饲粮中活加复合酶制剂对肉鸡生长的影响[J].浙江大学学报:农业与生命科学版,1999,25(6):615-618.

[8] BAREKATAIN M R,ANTIPATIS C,CHOCT M,et al.Interaction between protease and xylanase in broiler chicken diets containing sorghum distillers'dried grains with solubles[J].Animal Feed Science and Technology,2013,182(1/2/3/4):71-81.

[9] KALMENDAL R,TAUSON R.Effects of a xylanase and protease,individually or in combination,and an ionophorecoccidiostat on performance,nutrient utilization,and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet[J].Poultry Science,2012,91(6):1387-1393.  

[10] AURELI R,KLUENTER A M,FRU F,et al.Efficacy of protease supplementation on apparent ileal digestibility of nitrogen and amino acid of broiler chicken fed a diet based on 40% triticale[C]//Actes des 9èmes journées de la recherche avicole.Paris:Institut Technique de l'Aviculture,2011:384-387.

[11] YAN F,GARRIBAY L,ARCE J,et al.Effects of protease supplementation on broiler performance and in vitro protein digetibility[C]//Proceedings of the 23rd annual australian poultry science symposium.Sydney:Poultry Research Foundation,2012:134-137.

[12] GUGGENBUHL P,WACHÉ Y,WILSON J W.Effects of dietary supplementation with a protease on the apparent ileal digestibility of the weaned piglet[J].Journal of Animal Science,2012,90(S4):152-154.

[13] MC ALPINE P O,OSHEA C J,VARLEY P F,et al.The effect of protease and xylanase enzymes on growth performance and nutrient digestibility in finisher pigs[J].Journal of Animal Science,2012,90(S4):375-377.

[14] GHAZI S,ROOKE J A,GALBRAITH H.Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks[J].British Poultry Science,2003,44(3):410-418.  

[15] SELLE P H,LIU S Y,CAI J,et al.Steam-pelleting temperatures and grain variety of finely ground,sorghum-based broiler diets.Ⅰ.Influence on growth performance,relative gizzard weights,nutrient utilisation,starch and nitrogen digestibility[J].Animal Production Science,2013,53(3):339-346.

[16] 凌宝明,冯定远,左建军.饲用蛋白酶的研究进展[J].饲料工业,2009,30(22):7-10.

[17] 李慧.蛋白酶和木聚糖酶对肉鸡生长性能、消化机能及血液指标的影响[D].硕士学位论文.杨凌:西北农林科技大学,2010:55.

[18] GRACIA M I,LAZARO R,LATORRE M A,et al.Influence of enzyme supplementation of diets and cooking-flaking of maize on digestive traits and growth performance of broilers from 1 to 21 days of age[J].Animal Feed Science and Technology,2009,150(3/4):303-315.

[19] 袁玖.溢多酶对蛋鸡、肉鸡的生产效果及其作用机理研究[D].硕士学位论文.杨凌:西北农林科技大学,2006.

[20] PEEK H W,VAN DER KLIS J D,VERMEULEN B,et al.Dietary protease can alleviate negative effects of a coccidiosis infection on production performance in broiler chickens[J].Animal Feed Science and Technology,2009,150(1):151-159.
Outlines

/