Effects of Dietary &alpha;-Ketoglutarate on Heat Shock Protein 70 and Peroxisome Proliferator Activated Receptor &gamma; mRNA Expressions of Grass Carp (Ctenopharynodon idellus)

LIANG Jian; ZHAO Yurong; WANG Hongquan; JIN Baitao; TANG Deyue; WU Qin

doi:10.3969/j.issn.1006-267x.2014.05.032

2014 , Vol. 26 >Issue 5: 1379 - 1386

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.05.032

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Effects of Dietary α-Ketoglutarate on Heat Shock Protein 70 and Peroxisome Proliferator Activated Receptor γ mRNA Expressions of Grass Carp (Ctenopharynodon idellus)

LIANG Jian ,
ZHAO Yurong ,
WANG Hongquan ,
JIN Baitao ,
TANG Deyue ,
WU Qin

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College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China

Received date: 2013-11-14

Online published: 2014-05-05

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Abstract

This experiment was conducted to investigate the effects of α-ketoglutarate (AKG) on heat shock protein 70 (HSP70) and peroxisome proliferator activated receptor γ (PPARγ) mRNA expressions of grass carp (Ctenopharynodon idellus). A total of 450 grass carp with an average body weight of (35.66±0.39) g were randomly allotted to 5 groups with 3 replicates per group and 30 fish per replicate. Fish in control group were fed a basal diet, while those in experimental groups were fed experiemental diets supplemented with 0.25%, 0.50%, 0.75% and 1.00% AKG to substitute equivalent wheat middling on the basis of basal diet, respecitively. The experiment lasted for 60 days. An real-time quantitative PCR method was used to measure the relative expression levels of HSP70 and PPARγ mRNA in hepatopancreas, gill, kidney and spleen of grass carp at the end of experiment. The results showed as follows: there were differences in the relative expression levels of HSP70 and PPARγ mRNA among different tissues, and both of them were highly expressed in kindy. The relative expression level of HSP70 mRNA in kindy, hepatopancreas, spleen and gill was systematically decreased, while the relative expression level of PPARγ mRNA in kindy, hepatopancreas, gill and spleen was systematically decreased. AKG supplementation significantly affected the expressions of HSP70 and PPARγ mRNA in gill, kindy and spleen and the expression of HSP70 mRNA in hepatopancreas (P<0.05 or P<0.01). Compared with the control group, the relative expression levels of HSP70 and PPARγ mRNA in gill were significantly increased in the 1.00% AKG group (P<0.05 or P<0.01), and the relative expression levels of HSP70 and PPARγ mRNA in spleen were significantly increased in the 0.75% AKG group (P<0.05 or P<0.01), while the relative expression levels of HSP70 and PPARγ mRNA in kindy were significantly decreased in all AKG supplemental groups (P<0.05). The relative expression level of PPARγ mRNA in hepatopancreas in 0.25% AKG group was significantly higher than that in other groups (P<0.01), however, the relative expression level of HSP70 mRNA in hepatopancreas showed no significant difference among all groups (P>0.05). Based on above results, the recommended supplemental level of AKG in diets of grass carp (the body weight is about 35 g) is 0.75%.

Key words： grass carp; AKG; HSP70; PPARγ; expression

Cite this article

LIANG Jian , ZHAO Yurong , WANG Hongquan , JIN Baitao , TANG Deyue , WU Qin . Effects of Dietary α-Ketoglutarate on Heat Shock Protein 70 and Peroxisome Proliferator Activated Receptor γ mRNA Expressions of Grass Carp (Ctenopharynodon idellus)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(5) : 1379 -1386 . DOI: 10.3969/j.issn.1006-267x.2014.05.032

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