Aquaculture Nutrition

Dietary Biotin Requirement of Juvenile Pacific White Shrimp (Litopenaeus vannamei)

  • XIA Minghong ,
  • HUANG Xiaoling ,
  • WANG Hualang ,
  • MAI Kangsen ,
  • ZHOU Qicun
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  • 1. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    2. Aqua-Feed Research and Development Center, Guangdong Evergreen Feed Industry Co. , Ltd., Zhanjiang 524094, China;
    3. Laboratory of Aquatic Animal Nutrition and Feed, Ocean University of China, Qingdao 266003, China

Received date: 2013-12-18

  Online published: 2014-05-30

Abstract

An 8-week feeding trial was conducted to evaluate the effects of dietary biotin content on growth performance, body composition, biochemical indices of juvenile Pacific white shrimp (Litopenaeus vannamei), in order to estimate the dietary biotin requirement of juvenile Pacific white shrimp. Six isonitrogenous and isocaloric practical diets were formulated with graded dietary biotin contents of 0.068, 0.410, 0.910, 1.960, 4.620 and 9.380 mg/kg DM, respectively. Each diet was fed to one group of shrimps. Each group had 3 replicates and each replicate had 40 shrimps with the average body weight of 1.03 g. The results showed that weight gain rate (WGR), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) in 0.068 mg/kg DM group were all the lowest, and significantly lower than those in other groups (P0.05). No significant differences were observed in survival rate (SR), protein retention rate (PRR) and body composition among all groups (P>0.05). The contents of total protein (TP), glucose (GLU), total cholesterol (TC), triglyceride (TG) and malondialdehyde (MDA), and superoxide dismutase (SOD) activity in serum were not significantly affected by dietary biotin content (P>0.05). The highest serum total anti-oxidative capacity (T-AOC) was found in 0.910 mg/kg DM group, and significantly lower than that in 0.068 mg/kg DM group, but no significant differences were observed among other groups (P>0.05). The lowest serum catalase (CAT) activity was found in 0.068 mg/kg DM group, and significantly lower than that in other groups (P<0.05) except for 0.410 mg/kg DM group. The highest serum acetyl CoA carboxylase (ACC) activity was found in 4.620 mg/kg DM group, and significantly higher than that in 0.680, 0.410 and 0.910 mg/kg DM groups (P<0.05). Hepatopancreas biotin content was significantly increased with dietary biotin content increasing (P<0.05). The optimal dietary biotin requirements of juvenile Pacific white shrimp estimated using a broken-line model based on FE and serum ACC activity are 0.72 and 2.25 mg/kg, respectively.

Cite this article

XIA Minghong , HUANG Xiaoling , WANG Hualang , MAI Kangsen , ZHOU Qicun . Dietary Biotin Requirement of Juvenile Pacific White Shrimp (Litopenaeus vannamei)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1513 -1520 . DOI: 10.3969/j.issn.1006-267x.2014.06.011

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