Effects of Sodium Acetate and Sodium β-Hydroxybutyrate on Expressions of Genes Involved in Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

TA Na; LI Honglei; HOU Xianzhi; KAO Guilan; GAO Min; LI Dabiao

doi:10.3969/j.issn.1006-267x.2014.06.013

2014 , Vol. 26 >Issue 6: 1527 - 1534

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.013

Molecular Nutrition

Effects of Sodium Acetate and Sodium β-Hydroxybutyrate on Expressions of Genes Involved in Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells

TA Na ,
LI Honglei ,
HOU Xianzhi ,
KAO Guilan ,
GAO Min ,
LI Dabiao

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
3. Institute of Animal Nutrition, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2013-12-05

Online published: 2014-05-30

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Abstract

The aim of this study was to determine the effects of sodium acetate and sodium β-hydroxybutyrate on expressions of genes involved in milk fat and protein synthesis in bovine mammary epithelial cells (BMECs). The study was consisted of two parts, and completely random single-factor designs were adopted. Part 1: single supplementation test, the supplemental concentrations of sodium acetate and sodium β-hydroxybutyrate were 0 (control), 6.00, 9.00, 12.00 and 15.00 mmol/L, and 0 (control), 0.80, 1.60 and 2.40 mmol/L, respectively. Part 2: mixed supplementation test, based on data from part 1, the total supplemental concentration was the sum of optimal supplemental concentrations of sodium acetate and sodium β-hydroxybutyrate; three ratios of sodium acetate to sodium β-hydroxybutyrate (1:1, 2:1 and 4:1) were designed, and the treatment of no supplementation was used as a control group. The results showed as follows: 1) compared with control group, 12.00 mmol/L sodium acetate significantly increased the expression levels of acetyl-CoA carboxylase (ACC), fatty acid synthetase (FAS), diacylgycerol acyltransferase (DGAT), acetyl-CoA synthetase 2 (ACSS2), peroxisome prolifertor receptor (PPARG), κ-casein (CSN3) genes and the content of triglyceride (TAG) in BMECs (P<0.05). 2) Compared with control group, 2.40 mmol/L sodium β-hydroxybutyrate significantly increased the expression levels of ACC, FAS, ACSS2, PPARG and mammalian target of rapamycin (mTOR) genes and the content of TAG in BMECs (P<0.05). 3) When the ratios of sodium acetate to sodium β-hydroxybutyrate were 2:1 and 4:1, The supplementation of sodium acetate and sodium β-hydroxybutyrate at different mixed ratios significantly up-regulated the expression levels of genes involved in milk fat synthesis at different extents. The content of TAG in BMECs were significantly increased compared with when the ratio was 1:1 and control group (P<0.05). In conclusion, the mixed supplementation of 9.60 mmol/L sodium acetate and 4.80 mmol/L sodium β-hydroxybutyrate are optimal, considering its simulative effects on milk fat and protein synthesis.

Key words： bovine mammary epithelial cells; sodium acetate; sodium β-hydroxybutyrate; milk fat; milk protein

Cite this article

TA Na , LI Honglei , HOU Xianzhi , KAO Guilan , GAO Min , LI Dabiao . Effects of Sodium Acetate and Sodium β-Hydroxybutyrate on Expressions of Genes Involved in Milk Fat and Protein Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1527 -1534 . DOI: 10.3969/j.issn.1006-267x.2014.06.013

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