Application Effects of Recombinant Lactococcus lactis Expressing Porcine Lactoferrin of Weaner Piglets

HE Jia; HUANG Weiwei; MENG Qingsen; JIANG Yanping; CUI Wen; QIAO Xinyuan; GE Junwei; LIU Min; LI Yijing; TANG Lijie

doi:10.3969/j.issn.1006-267x.2014.06.019

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 6: 1579 - 1586

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.019

Feed Science and Technology

Application Effects of Recombinant Lactococcus lactis Expressing Porcine Lactoferrin of Weaner Piglets

HE Jia ,
HUANG Weiwei ,
MENG Qingsen ,
JIANG Yanping ,
CUI Wen ,
QIAO Xinyuan ,
GE Junwei ,
LIU Min ,
LI Yijing ,
TANG Lijie

Expand

College of Veterinary Medicine, Northeast Agriculture University, Harbin 150030, China

Received date: 2013-12-18

Online published: 2014-05-30

Fold

Abstract

This experiment was conducted to investigate the feasibility of recombinant Lactococcus lactis expressing porcine lactoferrin as an ecological friendly secure additive to substitute antibiotics feeding weaner piglets. A total of 36 weaner piglets with similar body weight were selected and randomly divided into 3 groups with 3 replicates in each group and 4 piglets in each replicate. The control group was fed a basal diet, the antibiotics group was fed the basal diet added 20 mg/kg flavomycin and 110 mg/kg chlortetracycline, while the trial group was fed the basal diet added recombinant Lactococcus lactis fermentation liquid (supplement according to the body weight of piglets, and the contain of porcine lactoferrin was 50 μg/kg per piglet per day) once a day. The experiment lasted for 21 days. The results showed that adding the recombinant Lactococcus lactis expressing porcine lactoferrin in the piglet diet, average daily gain was significantly higher than that in control group (P0.05); the content of interleukin-1 in serum was significantly higher than that in control group and antibiotics group (P<0.05), the content of interleukin-2 in serum was significantly higher than that in control group (P<0.01), and significantly higher than that in antibiotics group (P<0.05). Adding recombinant Lactococcus lactis expressing porcine lactoferrin in weaner piglet diets can promote the growth of piglet intestinal mucosal villus, improve piglet feed utilization rate, decrease the feed/gain, at the same time, strengthen the piglet immune function, and has the effect of improving piglet growth performance.

Key words： porcine lactoferrin; Lactococcus lactis; weaner piglets; instead of antibiotics

Cite this article

HE Jia , HUANG Weiwei , MENG Qingsen , JIANG Yanping , CUI Wen , QIAO Xinyuan , GE Junwei , LIU Min , LI Yijing , TANG Lijie . Application Effects of Recombinant Lactococcus lactis Expressing Porcine Lactoferrin of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1579 -1586 . DOI: 10.3969/j.issn.1006-267x.2014.06.019

References

[1] ISUI A G M,TANIA S C,SIGIFREDO A G,et al.Lactoferrin a multiple bioactive protein:an overview[J].Biochimica et Biophysica Acta-General Subjects,2012,1820(3):226-236.

[2] MULDER A M,CONNELLAN P A,OLIVER C J,et al.Bovine lactoferrin supplementation supports immune and antioxidant status in healthy human males[J].Nutrition Research,2008,28(9):583-589.

[3] HUNG C M,YEH C C,CHEN H L,et al.Porcine lactoferrin administration enhances peripheral lymphocyte proliferation and assists infectious bursal disease vaccination in native chickens[J].Vaccine,2010,28(16):2895-2902.

[4] HANSON L A,KOROTKOVA M,HAVERSEN L,et al.Breast-feeding,a complex support system for the offspring[J].Pediatrics International,2002,44(4):347-352.

[5] LEE W J,FARMER J L,HILTY M,et al.The protective effects of lactoferrin feeding against endotoxin lethal shock in germfree piglets[J].Infection and Immunity,1998,66(4):1421-1426.
[6] WANG Y Z,HAN F F,XU Z R.Developmental gene expression of lactoferrin in duodenum and effect of weaning age on gene expression of lactoferrin in piglets[J].Archives of Animal Nutrition,2006,60(1):1-9.

[7] HOHENSHELL L M,CUNNIK J E,FORD S P,et al.Few differences found between early and late weaned pigs raised in the same environment[J].Journal of Animal Science,2000,78(1):38-49.
[8] 哈卓,赵丽丽,于晓旭,等.重组猪乳铁蛋白N端的高效表达及抑菌活性检测[J].生物工程学报,2010,26(4):523-529.
[9] WANG Y Z,SHAN T Z,XU Z R,et al.Effects of the lactoferrin (LF) on the growth performance,intestinal microflora and morphology of weanling pigs[J].Animal Feed Science and Technology,2007,135(3/4):263-272.
[10] 魏秀秀,陈庆森,刘雪姬,等.乳铁蛋白对小鼠肠道微生物区系的影响研究[J].食品工业科技,2013,34(13):333-337.
[11] 张凯,方成堃,王冰,等.乳铁蛋白对早期断奶仔猪养分利用率及相关血液生化指标的影响[J].家畜生态学报,2012,33(4):47-51.
[12] 李美君,方成堃,张凯,等.饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响[J].动物营养学报,2012,24(1):111-116.
[13] 胡志和,李娜,刘传国,等.乳铁蛋白免疫增强作用评价[J].食品科学,2010,31(11):244-247.
[14] SFEIR R M,DUBARRY M,BOYAKA P N,et al.The mode of oral bovine lactoferrin administration influences mucosal and systemic immune responses in mice[J].The Journal of Nutrition,2004,134(2):403-409.
[15] 单体中,汪以真.重组猪乳铁蛋白(rPLF)对断奶仔猪血清IL-1、IL-2水平的影响[J].中国兽药杂志,2005,39(10):6-8.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References