Effects of Antimicrobial Peptide PR39 on Performance, Serum Immune Indexes and Intestinal Microflora of Weaner Piglets

LU Junxin; YANG Jinbo; KUANG Zheshi; ZHAO Xiangjie; YE Mingqiang; PAN Mushui

doi:10.3969/j.issn.1006-267x.2014.06.020

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 6: 1587 - 1592

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.020

Feed Science and Technology

Effects of Antimicrobial Peptide PR39 on Performance, Serum Immune Indexes and Intestinal Microflora of Weaner Piglets

LU Junxin ,
YANG Jinbo ,
KUANG Zheshi ,
ZHAO Xiangjie ,
YE Mingqiang ,
PAN Mushui

Expand

1. Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China;
2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2013-12-23

Online published: 2014-05-30

Fold

Abstract

This experiment was conducted to investigate the effects of antimicrobial peptide PR39 on performance, serum immune indexes and intestinal microflora of weaner piglets. A total of 120 weaner piglets at 28 days of age with the similar body weight were randomly allotted into 4 groups with 3 replicates per group and 10 piglets per replicate (half male and half female). The piglets in control group were fed a basal diet, those in experimental group Ⅰ were fed the basal diet+120 mg/kg kitasamycin, those in experimental group Ⅱ were fed the basal diet+200 mg/kg ntimicrobial peptide cecropin, and those in experimental group Ⅲ were fed the basal diet+300 mg/kg antimicrobial peptide PR39. The experiment lasted for 21 days. The results showed as follows: compared with control group, the two kinds of antimicrobial peptides both significantly increased average daily gain of piglets, while significantly reduced feed to gain ratio and diarrhea rate (P0.05). Compared with control group, the two kinds of antimicrobial peptides both significantly reduced the number of Escherichia coli in cecum, colon and rectum (P<0.05), while the number of Bifidobacterium in cecum in experimental group Ⅱ was significantly increased (P<0.05). In conclusion, the dietary supplementation of antimicrobial peptides can improve performance of weaner piglets, decrease the diarrhea rate, increase serum immunoglobulin contents, and stimulate beneficial bacteria proliferation, but inhibit pathogenic bacteria proliferation.

Key words： antimicrobial peptides; weaner piglets; performance; serum immune indexes; intestinal microflora

Cite this article

LU Junxin , YANG Jinbo , KUANG Zheshi , ZHAO Xiangjie , YE Mingqiang , PAN Mushui . Effects of Antimicrobial Peptide PR39 on Performance, Serum Immune Indexes and Intestinal Microflora of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1587 -1592 . DOI: 10.3969/j.issn.1006-267x.2014.06.020

References

[1] YEAMAN M R,YOUNT N Y.Mechanisms of antimicrobial peptide action and resistance[J].Pharmacological Reviews,2003,55(1):27-55.

[2] 宋青龙,王洪彬,张广民,等.日粮中添加天蚕素抗菌肽对仔猪健康及生产性能的影响[J].中国畜牧杂志,2012,48(13):52-55.
[3] 王菊芳.防御素抗菌肽对断奶仔猪生产性能的影响试验[J].湖南畜牧兽医,2012(3):9-10.
[4] 罗治华,周洪波,苏向东,等.抗菌肽饲料添加剂对断奶仔猪生产性能的影响[J].当代畜牧,2009(7):23-24.
[5] 陈香,李波,易学武,等.添加天蚕素抗菌肽对断奶仔猪生长性能的影响[J].中国饲料,2011(12):25-30.
[6] 刘扬科,李希,杨利,等.日粮中添加天蚕素抗菌肽对降低断奶仔猪饲用抗生素使用量的影响[J].饲料与畜牧,2012(8):56-58.
[7] 李永新,黄木家.抗菌肽制剂对仔猪生长性能和健康程度的影响[J].今日养猪业,2010(11):43-44.
[8] 谢红兵.复方中草药免疫增强剂对断奶仔猪免疫功能的影响[D].硕士学位论文.福州:福建农林大学,2004:30-31.
[9] TANG Z R,YIN Y L,ZHANG Y M,et al.Effects of dietary supplementation with an expressed fusion peptide bovine lactoferricin-lactoferrampin on performance,immune function and intestinal mucosal morpholopy in piglets weaned at age 21 d[J].British Journal of Nutrtion,2009,101(7):998-1005.

[10] 王阿荣,敖长金,宋青龙,等.日粮中添加天蚕素抗菌肽对断奶仔猪生产性能和血液生化指标的影响[J].饲料工业,2011,32(10):21-24.
[11] BAO H,SHE R,LIU T,et al.Effects of pig antibacterial peptides on growth performance and intestine mucosal immune of broiler chickens[J].Poultry Science,2009,88(2):291-297.

[12] 郭志强,杨奉珠,雷岷,等.抗菌肽对肉兔小肠黏膜形态、盲肠菌群和免疫功能的影响[J].动物营养学报,2012,24(9):1778-1784.
[13] WANG D,MA W,SHE R,et al.Effects of swine gut antimicrobial peptides on the intestinal mucosal immunity in specific-pathogen-free chickens[J].Poultry Science,2009,88(5):967-974.

[14] RADDYK V R,YEDERY R D,ARANHA C.Antimicrobial peptides:premises and promises[J].International Journal of Antimicrobial Agents,2004,24(6):536-547.

[15] CHEN X,ZHU F,CAO Y,et al.Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity[J].Antimicrobial Agents and Chemotherapy,2009,53(9):3683-3689.

[16] 张董燕,季海峰,刘辉,等.天蚕素抗菌肽对断奶仔猪生产性能及血清指标的影响[J].饲料研究,2011(10):22-24.
[17] 都明海,戚广州,王建军,等.抗菌脂肽对断奶仔猪生长性能、肠道微生物及血液指标的影响研究[J].中国粮油学报,2011,26(5):76-82.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References