Effects of Phytase on Performance, Metabolism of Calcium and Phosphorus and Intestinal Morphology of Layer Hens Fed Corn-Miscellaneous Meal Diets

LI Lianbin; CHEN Xiuli; YUE Hongyuan; GAO Yupeng; WU Shugeng

doi:10.3969/j.issn.1006-267x.2014.06.021

2014 , Vol. 26 >Issue 6: 1593 - 1600

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.021

Feed Science and Technology

Effects of Phytase on Performance, Metabolism of Calcium and Phosphorus and Intestinal Morphology of Layer Hens Fed Corn-Miscellaneous Meal Diets

LI Lianbin ,
CHEN Xiuli ,
YUE Hongyuan ,
GAO Yupeng ,
WU Shugeng

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1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
2. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2013-12-23

Online published: 2014-05-30

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Abstract

This experiment was conducted to study the effects of phytase supplemental level on performance, egg quality, metabolism of calcium and phosphorus and intestinal morphology of laying hens fed corn-miscellaneous meal diets. Six hundred Hy-Line Brown hens were randomly divided into 5 groups with 8 replicates per group and 15 laying hens per replicate. Positive control (PC) group was fed a basal diet containing 0.60% total phosphorus (0.36% available phosphorus), negative control (NC) group was fed the basal diet containing 0.40% total phosphorus (0.16% available phosphorus), and three experimental groups (P125 group, P250 group and P500 group) were fed the NC group diets supplemented with 125, 250, and 500 U/kg phytase, respectively. The experiment lasted for 24 weeks. The results showed as follows: 1) compared with NC group, laying rate, average egg weight and average daily feed intake in P250 group and P500 group were significantly increased (P0.05); there were no effects of phytase on feed/egg and egg quality (P>0.05). 2) Compared with NC group, tibia calcium and plasma calcium contents were not affected by dietary phytase (P>0.05), plasma phosphorus and tibia ash and phosphorus contents, and the apparent digestibility of phosphorus in P250 group and P500 group were significantly higher than those in NC group (P0.05). 3) Compared with NC group, the crypt depth in P250 group and P500 group was significantly decreased (P<0.05) and the villous height/crypt depth was significantly increased (P<0.05). In conclusion, the supplementation of phytase in corn-miscellaneous meal diets can improve performance, calcium and phosphorus digestibility and intestinal development of laying hens; the optimal level of phytase is 250 U/kg in the present study.

Key words： corn-miscellaneous meal diet; phytase; performance; metabolism of calcium and phosphorus; intestinal morphology

Cite this article

LI Lianbin , CHEN Xiuli , YUE Hongyuan , GAO Yupeng , WU Shugeng . Effects of Phytase on Performance, Metabolism of Calcium and Phosphorus and Intestinal Morphology of Layer Hens Fed Corn-Miscellaneous Meal Diets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1593 -1600 . DOI: 10.3969/j.issn.1006-267x.2014.06.021

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