Feed Science and Technology

Adhesion and Adhesion Antagonistic Effect of Live and Heat-Killed Lactobacillus acidophilus on IPEC-J2 Cells

  • YU Hanli ,
  • FANG Hua ,
  • CAI Xuan ,
  • XU Jianxiong
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  • 1. Shanghai Yuanyao Investment Co., Ltd., Shanghai 201316, China;
    2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received date: 2014-01-06

  Online published: 2014-05-30

Abstract

The adhesion and adhesion antagonistic effect of live and heat-killed Lactobacillus acidophilus on IPEC-J2 cells were studied in vitro. IPEC-J2 cells were treated with live and heat-killed Lactobacillus acidophilus, and then stained the cells and observed the adhesive property. Moreover, the inhibition ratio of live and heat-killed Lactobacillus acidophilus to inhibit the adhesion of Escherichia coli on IPEC-J2 cells through three treatments (competitive inhibition, exclusion inhibition and displacement inhibition) was assessed by eosin-methylene blue agar (EMB) culture medium. The results showed as follows: live and heat-killed Lactobacillus acidophilus all could adhere well to the IPEC-J2 cells. The number of heat-killed bacteria adhering to the per cell [(40±2) CFU] was higher than the number of live bacteria adhering to the per cell [(20±2) CFU]. The two forms of Lactobacillus acidophilus had a good ability to inhibit the Escherichia coli adhere to IPEC-J2 cells. In competitive and displacement inhibition experiments, live bacteria showed a significantly better inhibition ratio than heat-killed bacteria (P<0.05), whereas, in exclusion inhibition experiment, heat-killed bacteria showed a significantly better inhibition ratio than live bacteria (P<0.05). In conclusion, live and heat-killed Lactobacillus acidophilus can adhere well to the IPEC-J2 cells, and are able to reduce the adhesion and invasion to IPEC-J2 cells by Escherichia coli. Heat-killed Lactobacillus acidophilus inhibits Escherichia coli adhesion mainly through competition for host cell-binding receptors. Apart from competition for host cell-binding receptors, some antibacterial substances from live Lactobacillus acidophilus are able to inhibit the growth and reproduction of Escherichia coli.

Cite this article

YU Hanli , FANG Hua , CAI Xuan , XU Jianxiong . Adhesion and Adhesion Antagonistic Effect of Live and Heat-Killed Lactobacillus acidophilus on IPEC-J2 Cells[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1601 -1607 . DOI: 10.3969/j.issn.1006-267x.2014.06.022

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