Establishment of Techniques for Implanting Hepatic-Portal Catheters in Piglets for Studying Hepato-Nutrients Metabolism

ZHENG Peipei; BAO Zhengxi; LI Lulu; HU Changmin; ZHAO Shengjun; LIU Dongming; HUANG Feiruo

doi:10.3969/j.issn.1006-267x.2014.06.025

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 6: 1624 - 1631

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.025

Short Communications

Establishment of Techniques for Implanting Hepatic-Portal Catheters in Piglets for Studying Hepato-Nutrients Metabolism

ZHENG Peipei ,
BAO Zhengxi ,
LI Lulu ,
HU Changmin ,
ZHAO Shengjun ,
LIU Dongming ,
HUANG Feiruo

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1. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;
3. Wuhan University of Light Industry, Wuhan 430070, China

Received date: 2013-12-27

Online published: 2014-05-30

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Abstract

This study was aimed to establish techniques for implanting arterial and venous catheters in piglets to provide technical support for studying hepato-nutrients metabolism. A total of 18 healthy Duroc×Landrace×Yarkshire castrated piglets weighted (20.0±1.1) kg were selected, and hepatic vein, portal vein, mesenteric vein, carotid artery catheters were implanted respectively. After 5 days recovery period, a 1% solution of p-amino hippurate (pAH) was infused continuously into the mesenteric catheters. The blood samples from hepatic vein, portal vein and carotid artery were collected at 0.5 h before feeding and at 0.5, 1.5, 3.0, 5.0 and 7.5 h after feeding, respectively. Hepatic vein and portal vein blood flow were detected. The results showed that the average time of keeping unobstructed in blood vessels of the implanted catheter system was 16 days. Hepatic vein and portal vein blood flow velocity increased after feeding. Portal vein flowe reached 2.80 L/(kg·h) and 981.40 mL/min. Hepatic vein flow reached 4.04 L/(kg·h) and 1 414.65 mL/min. Twelve of the eighteen test piglets can be successfully used for studying hepato-nutrients metabolism. Hepatic vein and portal vein blood flow tends to similarly change.

Key words： piglet; liver metabolism; portal vein; hepatic vein; catheter techniques

Cite this article

ZHENG Peipei , BAO Zhengxi , LI Lulu , HU Changmin , ZHAO Shengjun , LIU Dongming , HUANG Feiruo . Establishment of Techniques for Implanting Hepatic-Portal Catheters in Piglets for Studying Hepato-Nutrients Metabolism[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1624 -1631 . DOI: 10.3969/j.issn.1006-267x.2014.06.025

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