Effects of Macleaya cordata Alkaloids on Intestinal Porcine Epithelial Cells Proliferation

LI Jie; WU Shusong; XIONG Xingyao; SU Dingding; HE Jianhua

doi:10.3969/j.issn.1006-267x.2014.06.026

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 6: 1632 - 1637

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.026

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Effects of Macleaya cordata Alkaloids on Intestinal Porcine Epithelial Cells Proliferation

LI Jie ,
WU Shusong ,
XIONG Xingyao ,
SU Dingding ,
HE Jianhua

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1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha 410128, China

Received date: 2013-12-13

Online published: 2014-05-30

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Abstract

To study the effects of sanguinarine, chelerythrine, protopine and allocryptopine from Macleaya cordata on intestinal porcine epithelial cells (IPEC-1) proliferation at different concentrations and different periods. IPEC-1 cells were pre-incubated for 24 hours, and then exposed to different concentrations (4, 2, 1, 0.5, 0.25, 0.1, 0.05, 0.025, 0.012 5 and 0.006 25 μg/mL of sanguinarine or chelerythrine, and 100, 50, 25, 12.5, 6.25, 3.125 and 1.562 5 μg/mL of protopine or allocryptopine; each treatment had 8 replicates) for additional 12 or 24 hours, respectively, cell viability was measured by MTT assay. The results showed as follows: after the IPEC-1 cells were treated by 0.25 to 4 μg/mL of sanguinarine, 0.25 to 4 μg/mL of chelerythrine, 100 μg/mL of protopine or 100 μg/mL of allocryptopine for 12 hours, the proliferation of cells was significantly inhibited (P0.05). The results indicate that the Macleaya cordata alkaloids can promote the proliferation of IPEC-1 under certain concentrations; it speculates that Macleaya cordata alkaloids may improve the intestinal digestive, absorptive, immune and barrier function through this way, and then improve the animal growth performance.

Key words： Macleaya cordata alkaloids; IPEC-1; MTT; proliferation

Cite this article

LI Jie , WU Shusong , XIONG Xingyao , SU Dingding , HE Jianhua . Effects of Macleaya cordata Alkaloids on Intestinal Porcine Epithelial Cells Proliferation[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1632 -1637 . DOI: 10.3969/j.issn.1006-267x.2014.06.026

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