Effects of Dried Alfalfa Hay on Ruminal Fermentation Parameters and Amino Acid Contents in Duodenal Chyme of Dorper&#215;Thin-Tailed Han Crossbred Sheep

XU Hong; YU Ying; LIANG Xinping; WANG Jie; GAO Yongge; WANG Chengzhang; YAN Xuebing

doi:10.3969/j.issn.1006-267x.2014.06.034

2014 , Vol. 26 >Issue 6: 1689 - 1697

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.06.034

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Effects of Dried Alfalfa Hay on Ruminal Fermentation Parameters and Amino Acid Contents in Duodenal Chyme of Dorper×Thin-Tailed Han Crossbred Sheep

XU Hong ,
YU Ying ,
LIANG Xinping ,
WANG Jie ,
GAO Yongge ,
WANG Chengzhang ,
YAN Xuebing

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College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2013-12-01

Online published: 2014-05-30

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Abstract

The purpose of this experiment was to investigate the effects of dried alfalfa hay on ruminal fermentation parameters and amino acid contents in duodenal chyme of Dorper×thin-tailed Han crossbred sheep. Five Dorper×thin-tailed Han crossbred sheep (F1 generation) with ruminal and duodenal fistulas were used in a 5×5 Latin square design. Five levels of dried alfalfa hay were designed, and its percentage in roughage was 0 (control group), 10% (test group Ⅰ), 20% (test group Ⅱ), 30% (test group Ⅲ) and 40% (test group Ⅳ), respectively. There were five test periods, each of them consisted of a 7-day pre-test period and a 10-day test period. The results showed as follows: 1) the percentage of dried alfalfa hay in roughage had no significant effects on rumen fluid pH and ammonia nitrogen content (P>0.05). 2) The content of urinary nitrogen in rumen fluid of different groups showed a similar tendency, except that test group Ⅰ was significantly higher than the other groups (P0.05). 3) With the increase of dried alfalfa hay percentage in roughage, the fermentation style in rumen was converted from propionic acid into acetic acid. 4) The contents of valine, methionine and leucine in duodenal chyme of test group Ⅰ and Ⅳ were significantly higher than those of control group (P0.05); the isoleucine content of test group Ⅰ was significantly higher than that of test group Ⅲ (P0.05). The above results indicate that dried alfalfa hay can improve ruminal fermentation, increase nitrogen utilization and the contents of some amino acids in duodenum; under conditions in the present study, the optimal percentage of dried alfalfa hay in roughage is 10%.

Key words： sheep; dried alfalfa hay; ruminal fermentation parameters; duodenum; amino acid

Cite this article

XU Hong , YU Ying , LIANG Xinping , WANG Jie , GAO Yongge , WANG Chengzhang , YAN Xuebing . Effects of Dried Alfalfa Hay on Ruminal Fermentation Parameters and Amino Acid Contents in Duodenal Chyme of Dorper×Thin-Tailed Han Crossbred Sheep[J]. Chinese Journal of Animal Nutrition, 2014 , 26(6) : 1689 -1697 . DOI: 10.3969/j.issn.1006-267x.2014.06.034

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