Effects of Maternal Folate Supplementation for Breeding Chickens on Lipid Metabolism-Related Gene Expression and Methylation in Offspring during Embryonic Development

YU Xiaoqiong; LIU Ranran; ZHAO Guiping; ZHENG Maiqing; LI Peng; WEN Jie

doi:10.3969/j.issn.1006-267x.2014.07.011

2014 , Vol. 26 >Issue 7: 1796 - 1806

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.011

Poultry Nutrition

Effects of Maternal Folate Supplementation for Breeding Chickens on Lipid Metabolism-Related Gene Expression and Methylation in Offspring during Embryonic Development

YU Xiaoqiong ,
LIU Ranran ,
ZHAO Guiping ,
ZHENG Maiqing ,
LI Peng ,
WEN Jie

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State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2014-02-20

Online published: 2014-07-01

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Abstract

This experiment was conducted to investigate the effects of maternal folate supplemental level for breeding chickens on hepatic lipid metabolism-related gene expression and methylation in offspring during embryonic development. Ninety 120-day-old Beijing-you chickens with similar body weight were randomly selected and divided into 3 groups with 30 replicates in each group and each replicate contained 1 chicken, and fed in single cages. Chickens were fed corn-soybean based diet (folic acid 0.53 mg/kg) supplemented with 0 (control group), 2.5 and 5.0 mg/kg folate for 90 days, respectively. The eggs were collected and hatched when the laying rate rose to the peak. The livers were collected on days 16 and 19 of embryonic development as well as at hatching. Folate concentrations in feed and egg were determined by competitive protein binding radioassay kits. The mRNA expression levels of several hepatic lipogenesis genes were analyzed by real-time quantitative polymerase chain reaction (RT-PCR). Bisulfite sequencing-polymerase chain reaction (BSP) and semi-nested polymerase chain reaction were used to determine the CpG methylation degree at the promoter region of sterol regulator element binding protein (SREBP-1c) ranging from -789 to -466 bp and fatty acid synthase (FAS) ranging from -159 to +143 bp in liver. The results showed as follows: adding 2.5 mg/kg folate in the diet had make egg folate content 81.92% higher than that in control group (P0.05). Folate supplementation significantly decreased the transcript abundance of the SREBP-1c among groups, besides, the rates in methylation of CpG sites in SREBP-1c among three periods were decreased by 8.54%, 9.47% and 8.37% between high and low folate supplementation groups, respectively. No significant differences were found in CpG methylation of SREBP-1c promoter among all groups in different periods (P>0.05). The expression of liver FAS showed the similar tendency with SREBP-1c, and reached the peak at day 1, on which the difference among groups was significant (P<0.05). Surprisingly, the rates in methylation of CpG sites at the FAS gene promoter region were highly hypomethylated. Interestingly, the ACC mRNA expression was exactly opposite direction with FAS, and the significant effect of folate on ACC expression level was observed at the embryonic age of 16 days(P<0.05). Overall, the results indicated that folate supplementation could significantly elevate yolk folate deposition (P<0.05). Meanwhile maternal folate levels could significantly influence the transcription of some lipogenesis genes (SREBP-1c, FAS and ACC) in offspring, but the methylated regions in the promoters of SREBP-1c and FAS were not associated with gene expressions. Folate level seemed no effects on alterations in DNA methylation among groups. The results why folate status have no significant changes with CpG methylation pattern of SREBP-1c and FAS may be that folate status seems to modulate other gene expression and DNA methylation, indirectly influencing the expression of SREBP-1c.

Key words： folate; laying hen; DNA methylation; gene expression; offspring

Cite this article

YU Xiaoqiong , LIU Ranran , ZHAO Guiping , ZHENG Maiqing , LI Peng , WEN Jie . Effects of Maternal Folate Supplementation for Breeding Chickens on Lipid Metabolism-Related Gene Expression and Methylation in Offspring during Embryonic Development[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 1796 -1806 . DOI: 10.3969/j.issn.1006-267x.2014.07.011

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