Effects of Molybdenum on Serum Anti-Oxidative Indices and Hepatic Apoptosis Gene Expressions of Dairy Goats Challenged by Cadmium

ZHUANG Yu; WANG Qi; CAO Huabin; HU Guoliang; LUO Junrong; GU Xiaolong

doi:10.3969/j.issn.1006-267x.2014.07.014

2014 , Vol. 26 >Issue 7: 1822 - 1829

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.014

Ruminant Nutrition

Effects of Molybdenum on Serum Anti-Oxidative Indices and Hepatic Apoptosis Gene Expressions of Dairy Goats Challenged by Cadmium

ZHUANG Yu ,
WANG Qi ,
CAO Huabin ,
HU Guoliang ,
LUO Junrong ,
GU Xiaolong

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Institution for the Surveillance and Control of Animal Population Disease, College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang 330045, China

Received date: 2014-02-25

Online published: 2014-07-01

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Abstract

This experiment was conducted to determine the effects of molybdenum (Mo) on serum anti-oxidative indices and hepatic apoptosis gene expressions of dairy goats challenged by cadmium (Cd). Thirty-six healthy Boer goats were randomly divided into 4 groups. Goats in test groups Ⅱ, Ⅲ and Ⅳ were all drenched 1 mg/kg BW Cd, and then were drenched 15, 30 and 45 mg/kg BW Mo, respectively, while those in test group Ⅰ were drenched demineralized water at the average dose of the other 3 groups. The experimental period was 50 days. Blood collection was carried out on days 0, 10, 20, 30, 40 and 50, then serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, nitric oxide (NO) content and NO synthase (NOS) activity were determined; liver tissue samples were obtained on days 0, 25 and 50 after slaughter, then hepatic Bax and Bcl-2 gene expression levels were determined. The results showed as follows: 1) compared with test group Ⅰ, serum T-AOC, SOD activity and hepatic Bcl-2 gene expression level could be significantly decreased in test groups Ⅱ, Ⅲ and Ⅳ (P<0.05), and serum NOS activity, MDA content, NO content and hepatic Bax gene expression level could be significantly increased in the above 3 groups (P<0.05); in addition, with the increase of experimental time, serum T-AOC, SOD activity and hepatic Bcl-2 gene expression level were significantly decreased (P<0.05), and serum MDA content, NO content, NOS activity and hepatic Bax gene expression level were significantly increased (P<0.05). 2) Compared among test groups Ⅱ, Ⅲ and Ⅳ, with the increase of Mo level, serum T-AOC and SOD activity were decreased, and serum MDA content, NO content and NOS activity and hepatic Bax gene expression level were increased when Cd were drenched at the same level. The results indicate that Mo and Cd can decrease serum antioxidant capacity and up-regulate the expressions of hepatic apoptosis genes, and they had a synergetic effect.

Key words： goat; cadmium; molybdenum; free radical; apoptosis gene

Cite this article

ZHUANG Yu , WANG Qi , CAO Huabin , HU Guoliang , LUO Junrong , GU Xiaolong . Effects of Molybdenum on Serum Anti-Oxidative Indices and Hepatic Apoptosis Gene Expressions of Dairy Goats Challenged by Cadmium[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 1822 -1829 . DOI: 10.3969/j.issn.1006-267x.2014.07.014

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