Effects of Dietary Soybean Meal Content on Hepatopancreas Structure and Function of Grass Carp (Ctenopharyngodon idellus)

CHEN Kequan; YE Yuantu; CAI Chunfang; HUANG Yuwei; LIU Hanchao; GONG Zhi; WU Tao; ZHANG Baotong; XIAO Peizhen

doi:10.3969/j.issn.1006-267x.2014.07.020

2014 , Vol. 26 >Issue 7: 1873 - 1879

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.020

Aquaculture Nutrition

Effects of Dietary Soybean Meal Content on Hepatopancreas Structure and Function of Grass Carp (Ctenopharyngodon idellus)

CHEN Kequan ,
YE Yuantu ,
CAI Chunfang ,
HUANG Yuwei ,
LIU Hanchao ,
GONG Zhi ,
WU Tao ,
ZHANG Baotong ,
XIAO Peizhen

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1. Key Laboratory of Aquatic Animal Nutrition in Jiangsu Province, Preclinical Medicine and Biological Science College of Soochow University, Suzhou 215123, China;
2. Laboratory of Aquatic Animal Nutrition Research System, Beijing Institute of Nutrition, Beijing 100069, China

Received date: 2014-02-17

Online published: 2014-07-01

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Abstract

In order to investigate the effects of dietary soybean meal content on hepatopancreas structure and function of grass carp (Ctenopharyngodon idellus), grass carp with an average body weight of (15.5±1.4) g were used as test object, and 4 isonitrogenous diets were designed which containing 0 (control group), 20%, 40% and 60% soybean meal, respectively. A total 240 grass carp were randomly divided into 4 groups with 3 replicates per group and 20 fish per replicate. After 6 weeks feeding in pond cages, the hepatopancreas function indexes in serum, hepatopancreas index and hepatopancreas lipid content of grass carp were examined, and the tissue structure of hepatopancreas was observed. The results showed as follows: the highest values of serum globulin (GLB), triglyceride (TG), high density lipoprotein (HDL) and cholesterol (TC) contents and alkaline phosphatase activity were all found in the 60% group, and there were significant differences between the 60% group and the other groups (P0.05), but the 60% group was the least. Hepatopancreas nuclear area in 60% group was the biggest, in addition to the 40% group, had no significant difference compared with the other groups (P>0.05). There was no significant difference in the ratio of nuclear volume and cell volume (Vn/Vc) among all groups (P>0.05), and the 40% group was minimum, the 60% group was penultimate. The results indicate that adding 60% soybean meal in diets of grass carp can cause the serum lipid and hepatopancreas lipid content increasing, and may lead to the injury of tissue structure and function of hepatopancreas.

Key words： grass carp (Ctenopharyngodon idellus); soybean meal; hepatopancreas; structure; function

Cite this article

CHEN Kequan , YE Yuantu , CAI Chunfang , HUANG Yuwei , LIU Hanchao , GONG Zhi , WU Tao , ZHANG Baotong , XIAO Peizhen . Effects of Dietary Soybean Meal Content on Hepatopancreas Structure and Function of Grass Carp (Ctenopharyngodon idellus)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 1873 -1879 . DOI: 10.3969/j.issn.1006-267x.2014.07.020

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