Residues and Organ Damage of Exogenous Gene and Protein from Transgenic Bt Brown Rice in Growing Pigs

CAO Zhenghui; WANG Zhanbin; GU Xianhong

doi:10.3969/j.issn.1006-267x.2014.07.024

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 7: 1908 - 1915

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.024

Feed Security

Residues and Organ Damage of Exogenous Gene and Protein from Transgenic Bt Brown Rice in Growing Pigs

CAO Zhenghui ,
WANG Zhanbin ,
GU Xianhong

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received date: 2014-01-13

Online published: 2014-07-01

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Abstract

This experiment was conducted to study the effects of transgenic Bacillus thuringiensis (Bt) brown rice on exogenous protein residues, exogenous gene residues and organ damage in growing pigs. Thirty Large White growing pigs with the body weight of (35.76±2.52) kg were randomly allocated into 2 treatments with 15 replicates per treatment. Pigs in control group were fed a basal diet + 70% non-transgenic Bt brown rice, and the pigs in test group were fed the basic diet + 70% transgenic Bt brown rice. The experiment lasted for 90 days. The results showed that dietary supplemented with transgenic Bt brown rice had no effects on the submicroscopic structure of the visceral organs of growing pigs. Exogenous protein and exogenous genes were not found in tissues organs, body fluids and excreta of growing pigs through polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) methods. The results suggest that supplemented 70% transgenic Bt brown rice in growing pig's diet, the exogenous genes and protein can be completely digested and absorbed by digestive tract without any residual in the body and will not damage the visceral organs.

Key words： Bt genes; brown rice; growing pigs; residues

Cite this article

CAO Zhenghui , WANG Zhanbin , GU Xianhong . Residues and Organ Damage of Exogenous Gene and Protein from Transgenic Bt Brown Rice in Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 1908 -1915 . DOI: 10.3969/j.issn.1006-267x.2014.07.024

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