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Effects of Heat Treatment of Soy Protein Isolate on Pancreatic Function of Broilers

  • CHEN Xing ,
  • WU Dawei ,
  • WEN Chao ,
  • ZHOU Yanmin
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  • College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

Received date: 2014-01-29

  Online published: 2014-07-01

Abstract

This experiment was conducted to investigate the effects of heat treatment soy protein isolate (SPI) on pancreatic function of broilers. A total of 320 one-day-old Arbor Acres broilers were randomly divided into 4 groups with 8 replicates per group and 10 broilers per replicate. Broilers were fed a diet contained normal SPI (control group), or diets contained SPI heated in an oven at 100℃ for 1, 4, and 8 h, respectively. The experiment lasted for 21 days. The results showed that compared with the control group, there were no significant differences in pancreatic function parameters of broilers fed diets contained heated SPI at 14 days of age (P>0.05). The pancreas relative weight of broilers fed diets contained heated SPI was significantly higher than that in control group at 21 days of age (P<0.05). The activities of protease and trypsin in pancreas were significantly lower in broilers fed diets contained heated SPI heated for 8 h than those in control group (P<0.05). Broilers fed diets contained SPI heated for 8 h exhibited a decrease in total antioxidant capacity (T-AOC) (P=0.058) and catalase (CAT) activity (P=0.064) compared with the control group at 21 days of age. Malondialdehyde (MDA) content of birds fed diets contained SPI heated for 8 h was significantly higher than that in control group at the same time (P<0.05). In addition, a tendency toward an increase in glutathione disulphide (GSSG) content (P=0.065) and an decrease in glutathione reductase (GR) activity (P=0.061) in broilers fed diets contained SPI heated for 8 h was observed at 21 days of age. Birds fed diets contained SPI heated for 4 and 8 h showed a significantly decrease in the ratio of reduced glutathione (GSH) to GSSG and glutathione peroxidase (GPx) activity than those in control group at 21 days of age (P<0.05). It is concluded that heated SPI ingestion after a specified period can increase the damage of pancreas function.

Cite this article

CHEN Xing , WU Dawei , WEN Chao , ZHOU Yanmin . Effects of Heat Treatment of Soy Protein Isolate on Pancreatic Function of Broilers[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 1942 -1949 . DOI: 10.3969/j.issn.1006-267x.2014.07.028

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