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Effects of 5 Kinds of Lactobacillus on Growth Performance, Body Composition, Serum Biochemical Indices and Intestinal Microflora of Tilapia (Oreochromis niloticus×O. aureu)

  • ZHOU Xiaobo ,
  • HUANG Yanhua ,
  • CAO Junming ,
  • KUANG Zheshi ,
  • WANG Guoxia ,
  • HUANG Wenqing ,
  • LIU Xiaoling
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  • 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    3. Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China

Received date: 2014-02-13

  Online published: 2014-07-01

Abstract

An 8-week experiment was conducted to investigate the effects of Lactobacillus with different strains in diets on growth performance, body composition, serum biochemical indices and intestinal flora of tilapia (Oreochromis niloticus×O. aureu). A total of 480 hybrid tilapia with an initial body weight of (3.49±0.01) g were randomly divided into 6 groups with 4 replicates per group and 20 fish per replicate. Among them, the fish in control group (group G0) were fed a basal diet, while those in the other 5 groups were fed the basal diets supplemented with Lactobacillus acidophilus (group G1), Lactobacillus plantarum (group G2), Lactobacillus pentosus (group G3), Lactobacillus rhamnosus (group G4) and Enterococcus faecalis (group G5), respectively. The number of Lactobacillus in diets from groups G0 to G5 was 1.30×103, 5.10×108, 2.97×108, 3.03×108, 2.30×108 and 3.47×108 CFU/g, respectively. The results showed as follows: at the end of the experiment, compared with group G0, the weight gain rate and specific growth rate in groups G2, G3 and G5 were significantly increased (P<0.05), the feed conversion ratio in groups G3 and G5 was significantly reduced (P<0.05), the whole body crude protein content in groups G3 and G4 was significantly increased (P<0.05), the whole body moisture content in group G3 was significantly decreased (P<0.05), and the serum cholesterol content in groups G1, G2, G4 and G5, serum triglyceride content in groups G4 and G5, serum urea nitrogen content in group G2, serum glucose content in group G4 and serum glutamic-pyruvic transaminase activity in groups G4 and G5 were all significantly decreased (P<0.05). Moreover, the number of intestinal Lactobacillus in groups G1, G2 and G5 and the number of intestinal Bacillus bifidus in group G1 were significantly higher than those in group G0 (P<0.05). Comprehensive analysis results indicate that the supplementation of Enterococcus faecalis show the best application effect for tilapia under the condition of this experiment. Adding Enterococcus faecalis in the diet can advance the growth, increase the feed utilization rate, decrease the contents of cholesterol and triglyceride and the activity of glutamic pyruvic transaminase in serum, raise the number of Lactobacillus in intestine, and improve the intestinal microenvironment of tilapia.

Cite this article

ZHOU Xiaobo , HUANG Yanhua , CAO Junming , KUANG Zheshi , WANG Guoxia , HUANG Wenqing , LIU Xiaoling . Effects of 5 Kinds of Lactobacillus on Growth Performance, Body Composition, Serum Biochemical Indices and Intestinal Microflora of Tilapia (Oreochromis niloticus×O. aureu)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 2009 -2017 . DOI: 10.3969/j.issn.1006-267x.2014.07.037

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