Short Communications

α-Linolenic Acid:Effects on Ruminal Fermentation Characteristics and the Intermediates of Bio-Hydrogenation

  • ZHOU Wei ,
  • XU Fanghua ,
  • CHEN Na ,
  • GAO Qingshan ,
  • LI Xiangzi
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  • Agricultural College of Yanbian University, Yanji 133000, China

Received date: 2014-01-01

  Online published: 2014-07-01

Abstract

This study was conducted to determine the effects of α-linolenic acid on ruminal fermentation characteristics and the intermediates of bio-hydrogenation. In vitro batch culture was applied, and α-linolenic acid was added to a basal substrate at levels of 0 (CON group), 1.5% (LL group), 3.0% (ML group) and 4.5% (HL group) (DM basis), respectively. Each group had three replicates. The results showed as follows: 1) ammonia nitrogen content, and the concentrations of total volatile fatty acids, acetic acid, butyric acid in ML and LL groups were lower than those in CON group at different degree, while propionic acid concentration was higher than that in CON group. 2) With the increase of culture time, c9,c12-C18:2 content in CON and LL groups was decreased, while that in other two groups was increased and ML group reached the maximum at 12 h, which was significantly higher than that in CON and LL groups (P<0.01). 3) c9,t11,c15-CLnA and c9,t13,c15-CLnA contents in HL group were significantly higher than those in the other groups at each time point (P<0.01). 4) Conjugated linoleic acid (CLA) and conjugated linolenic acid (CLnA) contents in ML and HL groups were significantly higher than those in the other groups at 12 h (P<0.01). Therefore, dietary supplementation of α-linolenic acid can change ruminal fermentation pattern, produce a variety of C18:1 isomers, promote the accumulation of t11,c15-C18:2, CLA and CLnA in rumen fluid, and there is dose-effect relationship between total CLnA and α-linolenic acid.

Cite this article

ZHOU Wei , XU Fanghua , CHEN Na , GAO Qingshan , LI Xiangzi . α-Linolenic Acid:Effects on Ruminal Fermentation Characteristics and the Intermediates of Bio-Hydrogenation[J]. Chinese Journal of Animal Nutrition, 2014 , 26(7) : 2018 -2025 . DOI: 10.3969/j.issn.1006-267x.2014.07.038

References

[1] LOURENCO M,RAMOS-MORALES E,WALLACE R J.The role of microbes in rumen lipolysis and biohydrogenation and their manipulation[J].Animal,2010,4(7):1008-1023.  
[2] HAFOOT C G,HAZLEWOOD G P.Lipid metabolism in the rumen[M]//HOBSON P N.The rumen microbial ecosystem.Netherlands:Springer,1998:382-426.
[3] POLAN C E,MCNEILL J J,TOVE C B.Biohydrogenation of unsaturated fatty acids by rumen bacteria[J].Journal of Bacteriology,1964,88(4):1056-1064.
[4] GARTON G A.Fatty acid metabolism in ruminants[J].Biochemistry of Lipids,1977,14:337-370.
[5] ABUGHAZALEH A A,RILEY M B,THIES E E,et al.Dilution rate and pH effects on the conversion of oleic acid to trans C18∶1 positional isomers in continuous culture[J].Journal of Dairy Science,2005,88(12):4334-4341.  
[6] JENKINS T C,ABUGHAZALEH A A,FREEMAN S,et al.The production of 10-hydroxystearic and 10-ketostearic acids is an alternative route of oleic acid transformation by the ruminal microbiota in cattle[J].The Journal of Nutrition,2006,136(4):926-931.
[7] ZHENG H C,LIU J X,YAO J H,et al.Effects of dietary sources of vegetable oils on performance of high-yielding lactating cows and conjugated linoleic acids milk[J].Journal of Dairy Science,2005,88(6):2037-2042.  
[8] WU Z,OHAJURUKA O A,PALMQUIST D L.Ruminal synthesis,biohydrogenation,and digestibility of fatty acids by dairy cows[J].Journal of Dairy Science,1991,74(9):3025-3034.  
[9] AVILA C D,DEPETERS E J,PEREZ-MONTI H,et al.Influences of saturation ratio of supplemental dietary fat on digestion and milk yield in dairy cows[J].Journal of Dairy Science,2000,83(7):1505-1519.  
[10] 孙攀峰,姚建红,刘建新.瘤胃十八碳不饱和脂肪酸氢化的研究进展[J].动物营养学报,2007,19(增刊):508-514.
[11] PARK Y,STORKSON J M,ALBRIGHT K J,et al.Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice[J].Lipids,1999,34(3):235-241.  
[12] AZAIN M J,HAUSMAN D B,SISK M B,et al.Dietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number[J].The Journal of Nutrition,2000,130(6):1548-1554.
[13] IGARASHI M,MIYAZAWA T.Newly recognized cytotoxic effect of conjugated trienoic fatty acids on cultured human tumor cells[J].Cancer Letters,2000,148(1):173-179.
[14] WASOWSKA I,MAIA M R G,NIEDZWIEDZKA K M,et al.Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids[J].British Journal of Nutrition,2006,95(6):1199-1211.  
[15] CHOI S H,SONG M K.Effect of C18-polyunsaturated fatty acids on their direct incorporation into the rumen bacterial lipids and CLA production in vitro[J].Asian-Australasian Journal of Animal Sciences,2005,18(4):1011-2367.
[16] MCDOUGALL E I.Studies on ruminant saliva.1.The composition and output of sheep's saliva[J].Biochemical Journal,1948,43(1):99-109.
[17] FOLCH J,LEES M,SLOANE STANLEY G H.A simple method for the isolation and purification of total lipides from animal tissues[J].The Journal of Biological Chemistry,1957,226(1):497-509.
[18] LEPAGE G,ROY C C.Direct transesterification of all classes of lipids in a one-step reaction[J].Journal of Lipid Research,1986,27(1):114-220.
[19] 张春梅,易贤武,苑志朋,等.富含十八碳不饱和脂肪酸的植物油对体外瘤胃发酵和甲烷生成的影响[J].畜牧与兽医,2009,41(12):16-19.
[20] ZHANG C M,WU Y W,YUAN Z P,et al.Effects of adding mixtures of linoleic acid and linolenic acid with different proportions on rumen fermentation and methanogenesis in vitro[J].Chinese Journal of Animal Nutrition,2008,20(2):223-227.
[21] 刘亮.体外法研究苹果酸和十八碳脂肪酸对瘤胃脂肪酸代谢及甲烷产量的影响 .硕士学位论文.北京:中国农业科学院,2008.
[22] 魏宏阳,王加启.添加游离不饱和脂肪酸或植物油对瘤胃微生物生物体外发酵及微晶纤维素降解的影响[J].畜牧兽医学报,2004,35(5):491-497.
[23] 周倩,刘佩,李海霞,等.微生物合成共轭亚油酸机理的研究进展[J].食品工业科技,2011(11):468-471,474.
[24] SONG M K,WANG J H,SON Y S,et al.Addition effect of seed-associated or free linseed oil on the formation of cis-9,trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro[J].Asian-Australasian Journal of Animal Science,2002,15(8):1115-1120.
[25] KIM Y J,LIU R H,BOND D R,et al.Effect of linoleic acid concentration on conjugated linoleic acid production by Butyrivibrio fibrisolvens A38[J].Applied and Environmental Microbiology,2000,66(12):5226-5230.  
[26] HUGHES P E,HUNTER W J,TOVE S B.Biohydrogenation of unsaturated fatty acids.Purification and properties of cis-9,trans-11-octadecadienoate reductase[J].Journal of Biological Chemistry,1982,257(7):3643-3649.
[27] 李香子.调控瘤胃发酵对甲烷及共轭脂肪酸(CLA,CLnA)生成机制影响研究 .博士学位论文.兰州:甘肃农业大学,2009.
[28] 郜玉婷,叶淑红,王际辉,等.保加利亚乳杆菌利用α-亚麻酸合成共轭亚麻酸[J].大连工业大学学报,2012,31(4):235-238.
[29] 张春梅,苑志明,易贤武,等.亚麻酸对饲喂不同精粗比日粮的湖羊瘤胃细菌区系的影响[J].饲料工业,2013,34(11):46-49.
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