Effects of Tributyrin and Mannan-Oligosaccharide on Growth Performance, Body Composition and Intestinal Health Indices of Tawny Puffer (Takifugu flavindus)

ZHAI Qiuling, ZHANG Chunxiao, SUN Yunzhang, WANG Ling, SONG Kai, ZHANG Lu

doi:10.3969/j.issn.1006-267x.2014.08.022

2014 , Vol. 26 >Issue 8: 2197 - 2208

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.08.022

Aquaculture Nutrition

Effects of Tributyrin and Mannan-Oligosaccharide on Growth Performance, Body Composition and Intestinal Health Indices of Tawny Puffer (Takifugu flavindus)

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1. Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Fisheries College of Jimei University, Xiamen 361021, China;
2. Tongwei Co., Ltd., Chengdu 610041, China

Received date: 2014-03-10

Online published: 2014-08-13

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Abstract

This experiment was conducted to investigate the effects of tributyrin (TB) and mannan-oligosaccharides (MOS) on growth performance, body composition and intestinal health indices of tawny puffer (Takifugu flavindus). A basal diet was formulated as control, and 5 experimental diets were formulated by adding 50 mg/kg TB, 100 mg/kg TB, 150 mg/kg TB, 50 mg/kg TB+250 mg/kg MOS and 100 mg/kg TB+250 mg/kg MOS in the basal diet, respectively. A total of 360 tawny puffer with an initial body weight of (15.99±0.15) g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The experiment lasted for 8 weeks. The results showed that weight gain rate (WGR), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) of tawny puffer were firstly increased with the TB supplemental level increasing, and the biggest values of them were all found in 100 mg/kg group, and then changed to be steady. Compared with 50 mg/kg TB group, the WGR, SGR, FE and PER of tawny puffer in 50 mg/kg TB+250 mg/kg MOS group were significantly increased (P<0.05). Moreover, the WGR in 50 mg/kg TB+250 mg/kg MOS group was significantly higher than that in 100 mg/kg TB+250 mg/kg MOS group (P<0.05). Hepatopancreas crude lipid content in combined adding groups was significantly increased compared with single adding groups with the same TB supplemental level (P<0.05), while the hepaticglycogen content was significantly decreased(P<0.05), and the lowest value was found in 100 mg/kg TB+250 mg/kg MOS group. Intestinal protease activity in 50 mg/kg TB+250 mg/kg MOS group was significantly higher than that in other groups (P<0.05). Compared with 50 mg/kg TB group, serum total protein and glucose contents were significantly increased (P<0.05), while serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities were significantly decreased (P<0.05). The number of intestinal aerobic marine heterotrophic bacteria in 50 mg/kg TB+250 mg/kg MOS group was significantly increased compared with 50 mg/kg TB group (P<0.05), while that in 100 mg/kg TB+250 mg/kg MOS group was significantly decreased compared with 100 mg/kg TB group (P<0.05). Compared with control group, the number of intestinal Vibrio spp. in 50 mg/kg TB+250 mg/kg MOS group was significantly increased (P<0.05), while the number of intestinal Lactobacillus was significantly decreased (P<0.05). Villus height and width of proximal and distal intestines in combined adding groups were all higher than those in single adding groups with the same TB supplemental level, but the differences were not significant (P>0.05). The results indicate that adding 50 mg/kg TB+250 mg/kg MOS in the diet can maintenance the intestinal health, strength the intestinal digestive activities and increase the growth performance of tawny puffer.

Key words： tributyrin; mannan-oligosaccharides; tawny puffer (Takifugu flavindus); growth performance; body composition; intestinal microflora; intestinal structure

Cite this article

ZHAI Qiuling, ZHANG Chunxiao, SUN Yunzhang, WANG Ling, SONG Kai, ZHANG Lu . Effects of Tributyrin and Mannan-Oligosaccharide on Growth Performance, Body Composition and Intestinal Health Indices of Tawny Puffer (Takifugu flavindus)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(8) : 2197 -2208 . DOI: 10.3969/j.issn.1006-267x.2014.08.022

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