Feed Science and Technology

Protective Effects of Selenium on aflatoxin B1 Induced Hepatic Oxidative Stress in Broilers

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  • College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2014-03-03

  Online published: 2014-08-13

Abstract

To investigate the protective effects of selenium(Se) on aflatoxin B1 (AFB1) exposed broilers and its probable mechanism, ninety one-day-old broilers were randomly allotted to 3 groups (3 replicates per group and 10 birds per replicate): basal diet group (control group), basal diet+100 μg/kg AFB1 group (AFB1 group), basal diet 100 μg/kg AFB1 +0.3 mg/kg Se group (Se group). The trial lasted for 42 days. The results showed as follows: 1) compared with control group, the broilers in AFB1 group had a significantly lower liver weight (P<0.05), and serum glutamic pyruvic transaminase (GPT) activity was significantly increased (P<0.05). The contents of maleic dialdehyde (MDA) and H2O2 in AFB1 group were higher than those in control group (P<0.05), but total superoxide dismutase (T-SOD) activity, glutathione (GSH) content and glutathione peroxidase (GSH-Px) activity were significantly decreased (P<0.05). Multiple bleeding sites and hepatocyte necrosis and atrophy were observed by liver histopathology in AFB1 group. Moreover, the expression of NF-E2-related factor 2 (Nrf2) (P<0.05) and caspase-3 were increased in hepatocyte nucleus of AFB1 exposed broilers. 2) Compared with AFB1 group, the liver function indexes were improved by Se addition. Hepatic damage was reduced while Nrf2 expression in hepatocyte nucleus was decreased (P<0.05). These results suggest that Se could attenuate liver dysfunction and oxidative damage, improve performance, increase antioxidant ability in the AFB1 exposed broilers.

Cite this article

RUI Xiaoli, CHEN Sitan, LI Chunmei . Protective Effects of Selenium on aflatoxin B1 Induced Hepatic Oxidative Stress in Broilers[J]. Chinese Journal of Animal Nutrition, 2014 , 26(8) : 2281 -2288 . DOI: 10.3969/j.issn.1006-267x.2014.08.032

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