This experiment was conducted to develop a low-cost yeast liquid medium which was used in industrial production and had a good growth effect. In this study, eight kinds of local agricultural products were used as fermentation substrate. The components of the medium were designed by uniform design and the yeast viable count in the liquid medium after fermentation and the content of yeast polysaccharide was determined to select the best carbon source and nitrogen source. Then the composition of the liquid medium was determined by measuring the ratio of composite carbon source and composite nitrogen source and inorganic salts addition. The results showed that the composition of the yeast liquid culture included anhydrous glucose (0.9%), molasses (4.5%), wheat bran (3.6%), corn distillers dried grains with solubles (0.3%), cottonseed meal (0.275%), yeast extract (0.075%) and (NH₃)₂SO₄ (0.08%).The inorganic salts included KH₂PO₄ (0.3%), MgSO₄ (0.03%) and CaCl₂ (0.03%). The viable count was 2.38×10⁸ CFU/mL and the contents of β-glucan and mannan were 651.6 and 210.8 mg/L. In conclusion, the yeast liquid medium is not only good for the yeast rapid growth and reproduction in the industrial production but also saves raw materials costs.